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IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Characterization of cAMP accumulation mediated by three alpha(1)-adrenoceptor subtypes in HEK293 cells
作者: Song, Y; Huang, Y; Dong, ED; Han, QD; Zhang, YY
关键词: alpha-1 adrenergic receptors ; cyclic AMP ; HEK293 cells ; radioligand assay
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2003-06-01
卷: 24, 期:6, 页:549-554
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: RAT CEREBRAL-CORTEX ; ADRENERGIC-RECEPTORS ; ADENOSINE ; PATHWAYS ; MONOPHOSPHATE ; HEPATOCYTES ; SLICES ; BRAIN
英文摘要:

AIM: To investigate the characterization of cAMP response mediated by alpha(1)-adrenoceptor (alpha(1)-AR) subtypes in HEK293 cells. METHODS: (1) Full-length cDNA encoding three alpha(1)-AR subtypes were transfected into HEK293 cells by the calcium phosphate precipitation method, respectively. (2) The densities of alpha(1)-AR subtypes expressed in HEK293 cells were measured by radioligand binding assay. (3) cAMP accumulation was measured by [H-3] adenine prelabeling method. RESULTS: (1) Activation of each of three subtypes resulted in an increase of cAMP accumulation in HEK293 cells in a dose-dependent manner, which was inhibited by selective alpha(1)-AR antagonist prazosin. (2) Comparing the pharmacological property, the maximal responses of alpha(1A)-AR to agonists were the most potent, while the sensitivity of alpha(1)-AR subtypes to norepinephrine (NE) was the highest. CONCLUSION: Each of three alpha(1)-AR subtypes can mediate cAMP accumulation in HEK293 cell line, and there are differences in pharmacological property.

语种: 英语
WOS记录号: WOS:000183575100010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67816
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: Peking Univ, Hosp 3, Inst Vasc Med, Reference Lab,Minist Educ Mol Cardiovasol, Beijing 100083, Peoples R China

Recommended Citation:
Song, Y,Huang, Y,Dong, ED,et al. Characterization of cAMP accumulation mediated by three alpha(1)-adrenoceptor subtypes in HEK293 cells[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):549-554.
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