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Characterization of cAMP accumulation mediated by three alpha(1)-adrenoceptor subtypes in HEK293 cells
Song, Y; Huang, Y; Dong, ED; Han, QD; Zhang, YY
关键词alpha-1 adrenergic receptors cyclic AMP HEK293 cells radioligand assay
刊名ACTA PHARMACOLOGICA SINICA
2003-06-01
24期:6页:549-554
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]RAT CEREBRAL-CORTEX ; ADRENERGIC-RECEPTORS ; ADENOSINE ; PATHWAYS ; MONOPHOSPHATE ; HEPATOCYTES ; SLICES ; BRAIN
英文摘要

AIM: To investigate the characterization of cAMP response mediated by alpha(1)-adrenoceptor (alpha(1)-AR) subtypes in HEK293 cells. METHODS: (1) Full-length cDNA encoding three alpha(1)-AR subtypes were transfected into HEK293 cells by the calcium phosphate precipitation method, respectively. (2) The densities of alpha(1)-AR subtypes expressed in HEK293 cells were measured by radioligand binding assay. (3) cAMP accumulation was measured by [H-3] adenine prelabeling method. RESULTS: (1) Activation of each of three subtypes resulted in an increase of cAMP accumulation in HEK293 cells in a dose-dependent manner, which was inhibited by selective alpha(1)-AR antagonist prazosin. (2) Comparing the pharmacological property, the maximal responses of alpha(1A)-AR to agonists were the most potent, while the sensitivity of alpha(1)-AR subtypes to norepinephrine (NE) was the highest. CONCLUSION: Each of three alpha(1)-AR subtypes can mediate cAMP accumulation in HEK293 cell line, and there are differences in pharmacological property.

语种英语
WOS记录号WOS:000183575100010
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67816
专题北京大学第三临床医学院_心血管内科
作者单位Peking Univ, Hosp 3, Inst Vasc Med, Reference Lab,Minist Educ Mol Cardiovasol, Beijing 100083, Peoples R China
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GB/T 7714
Song, Y,Huang, Y,Dong, ED,et al. Characterization of cAMP accumulation mediated by three alpha(1)-adrenoceptor subtypes in HEK293 cells[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):549-554.
APA Song, Y,Huang, Y,Dong, ED,Han, QD,&Zhang, YY.(2003).Characterization of cAMP accumulation mediated by three alpha(1)-adrenoceptor subtypes in HEK293 cells.ACTA PHARMACOLOGICA SINICA,24(6),549-554.
MLA Song, Y,et al."Characterization of cAMP accumulation mediated by three alpha(1)-adrenoceptor subtypes in HEK293 cells".ACTA PHARMACOLOGICA SINICA 24.6(2003):549-554.
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