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Comparative Analysis of the Major Constituents in the Traditional Tibetan Medicinal Plants Saussurea laniceps and S. medusa by LC-DAD-MS
Yi, Tao1; Chen, Hu-Biao1; Zhao, Zhong-Zhen1; Jiang, Zhi-Hong1; Cai, Shao-Qing2; Wang, Tian-Min2
关键词Column liquid chromatography LC-DAD-MS Traditional Tibetan medicine Xuelianhua Saussurea laniceps and Saussurea medusa
刊名CHROMATOGRAPHIA
2009-09-01
DOI10.1365/s10337-009-1240-7
70期:5-6页:957-962
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]GLYCOSIDES
英文摘要

Liquid chromatography coupled with diode array detector and electrospray ionization mass spectrometry was developed for the qualitative and quantitative comparison of the main constituents in Saussurea laniceps (SL) and S. medusa (SM), two species of plants used under the name "Xuelianhua" in traditional Tibetan medicine. A method validation including linearity, limit of detection, precision and recovery was performed. The results showed that a good linearity with R (2) > 0.99 was achieved, and the limit of detection of the quantified constituents was reported to be between 0.8 and 3.3 ng. The relative standard deviation value was below 3.82% for repeatability, and recovery studies for the quantified compounds were found to be within the range 90.92-103.12%. The unique properties of the present method were evaluated by analyzing twelve related herbal samples including five S. laniceps samples and seven S. medusa samples. Twenty-two compounds including phenolic acids, cumarins, lignanoids and flavonoids were identified by online ESI-MS and by comparison with literature data and standard compounds, and seven of them were quantified by LC-DAD simultaneously. The results demonstrated that the common constituents in the two herbs were protocatechuic acid, syringoside, chlorogenic acid, isoquercitroside, 1,5-dicaffeoylquinic acid, apigenin 7-O-beta-d-glucoside, chrysoeriol 7-O-beta-d-glucoside, acacetin 7-O-beta-d-glucoside, apigenin and chrysoeriol. In the present study, it was found that the characteristic constituents were umbelliferone, scopoletin and their glucosides in S. laniceps, as well as arctiin and arctigenin in S. medusa. It was feasible to choose these characteristic compounds for the quality evaluation as well as chemical authentication of the two related herbs. The results also support discrimination between the two species when using them in folk medicine.

语种英语
WOS记录号WOS:000269879000039
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67833
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位1.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Yi, Tao,Chen, Hu-Biao,Zhao, Zhong-Zhen,et al. Comparative Analysis of the Major Constituents in the Traditional Tibetan Medicinal Plants Saussurea laniceps and S. medusa by LC-DAD-MS[J]. CHROMATOGRAPHIA,2009,70(5-6):957-962.
APA Yi, Tao,Chen, Hu-Biao,Zhao, Zhong-Zhen,Jiang, Zhi-Hong,Cai, Shao-Qing,&Wang, Tian-Min.(2009).Comparative Analysis of the Major Constituents in the Traditional Tibetan Medicinal Plants Saussurea laniceps and S. medusa by LC-DAD-MS.CHROMATOGRAPHIA,70(5-6),957-962.
MLA Yi, Tao,et al."Comparative Analysis of the Major Constituents in the Traditional Tibetan Medicinal Plants Saussurea laniceps and S. medusa by LC-DAD-MS".CHROMATOGRAPHIA 70.5-6(2009):957-962.
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