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学科主题基础医学
The type VI secretion system gene cluster of Salmonella typhimurium: Required for full virulence in mice
Liu, Ji1,2; Guo, Ji-Tao3; Li, Yong-Guo4; Johnston, Randal N.5; Liu, Gui-Rong1; Liu, Shu-Lin1,2,3,4,6
关键词Bacterial secretion systems Mouse model Salmonella typhimurium T6SS Virulence
刊名JOURNAL OF BASIC MICROBIOLOGY
2013-07-01
DOI10.1002/jobm.201200047
53期:7页:600-607
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]ENTERICA SEROVAR TYPHIMURIUM ; INTRACELLULAR REPLICATION ; III SECRETION ; HOST-CELLS ; PROTEIN ; GENOME ; IDENTIFICATION ; BACTERIA ; INFECTIONS ; CONTRIBUTE
英文摘要

Type VI secretion system (T6SS) has increasingly been believed to participate in the infection process for many bacterial pathogens, but its role in the virulence of Salmonella typhimurium remains unclear. To look into this, we deleted the T6SS cluster from the genome of S. typhimurium 14028s and analyzed the phenotype of the resulting T6SS knockout mutant (T6SSKO mutant) in vitro and in vivo. We found that the T6SSKO mutant exhibited reduced capability in colonizing the spleen and liver in an in vivo colonization competition model in BALB/ c mice infected by the oral route. Additionally, infection via intraperitoneal administration also showed that the T6SSKO mutant was less capable of colonizing the mouse spleen and liver than the wild-type strain. We did not detect significant differences between the T6SSKO and wild-type strains in epithelial cell invasion tests. However, in the macrophage RAW264.7 cell line, the T6SSKO mutant survived and proliferated significantly more poorly than the wild-type strain. These findings indicate that T6SS gene cluster is required for full virulence of S. typhimurium 14028s in BALB/ c mice, possibly due to its roles in bacterial survival and proliferation in macrophages.

语种英语
WOS记录号WOS:000323227300007
项目编号NSFC30870098 ; 30970119 ; 81030029 ; NSFC30970078 ; NSFC-NIH 81161120416 ; 20092307110001
资助机构Harbin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health of USA ; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) ; Natural Science Foundation of Heilongjiang Province of China
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67857
专题北京大学基础医学院_病原生物学系
作者单位1.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
2.Genom Res Ctr, Harbin, Peoples R China
3.Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB, Canada
4.Harbin Med Univ, Dept Biopharmaceut, Fac Pharm, Harbin 150081, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
6.Harbin Med Univ, Affiliated Hosp 1, Genet Diag Ctr, Harbin 150081, Peoples R China
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GB/T 7714
Liu, Ji,Guo, Ji-Tao,Li, Yong-Guo,et al. The type VI secretion system gene cluster of Salmonella typhimurium: Required for full virulence in mice[J]. JOURNAL OF BASIC MICROBIOLOGY,2013,53(7):600-607.
APA Liu, Ji,Guo, Ji-Tao,Li, Yong-Guo,Johnston, Randal N.,Liu, Gui-Rong,&Liu, Shu-Lin.(2013).The type VI secretion system gene cluster of Salmonella typhimurium: Required for full virulence in mice.JOURNAL OF BASIC MICROBIOLOGY,53(7),600-607.
MLA Liu, Ji,et al."The type VI secretion system gene cluster of Salmonella typhimurium: Required for full virulence in mice".JOURNAL OF BASIC MICROBIOLOGY 53.7(2013):600-607.
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