北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
The type VI secretion system gene cluster of Salmonella typhimurium: Required for full virulence in mice
作者: Liu, Ji1,2; Guo, Ji-Tao3; Li, Yong-Guo4; Johnston, Randal N.5; Liu, Gui-Rong1; Liu, Shu-Lin1,2,3,4,6
关键词: Bacterial secretion systems ; Mouse model ; Salmonella typhimurium ; T6SS ; Virulence
刊名: JOURNAL OF BASIC MICROBIOLOGY
发表日期: 2013-07-01
DOI: 10.1002/jobm.201200047
卷: 53, 期:7, 页:600-607
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: ENTERICA SEROVAR TYPHIMURIUM ; INTRACELLULAR REPLICATION ; III SECRETION ; HOST-CELLS ; PROTEIN ; GENOME ; IDENTIFICATION ; BACTERIA ; INFECTIONS ; CONTRIBUTE
英文摘要:

Type VI secretion system (T6SS) has increasingly been believed to participate in the infection process for many bacterial pathogens, but its role in the virulence of Salmonella typhimurium remains unclear. To look into this, we deleted the T6SS cluster from the genome of S. typhimurium 14028s and analyzed the phenotype of the resulting T6SS knockout mutant (T6SSKO mutant) in vitro and in vivo. We found that the T6SSKO mutant exhibited reduced capability in colonizing the spleen and liver in an in vivo colonization competition model in BALB/ c mice infected by the oral route. Additionally, infection via intraperitoneal administration also showed that the T6SSKO mutant was less capable of colonizing the mouse spleen and liver than the wild-type strain. We did not detect significant differences between the T6SSKO and wild-type strains in epithelial cell invasion tests. However, in the macrophage RAW264.7 cell line, the T6SSKO mutant survived and proliferated significantly more poorly than the wild-type strain. These findings indicate that T6SS gene cluster is required for full virulence of S. typhimurium 14028s in BALB/ c mice, possibly due to its roles in bacterial survival and proliferation in macrophages.

语种: 英语
所属项目编号: NSFC30870098 ; 30970119 ; 81030029 ; NSFC30970078 ; NSFC-NIH 81161120416 ; 20092307110001
项目资助者: Harbin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institutes of Health of USA ; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) ; Natural Science Foundation of Heilongjiang Province of China
WOS记录号: WOS:000323227300007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67857
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
The type VI secretion system gene cluster of Salmonella typhimurium Required for full virulence in mice.pdf(218KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
2.Genom Res Ctr, Harbin, Peoples R China
3.Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB, Canada
4.Harbin Med Univ, Dept Biopharmaceut, Fac Pharm, Harbin 150081, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
6.Harbin Med Univ, Affiliated Hosp 1, Genet Diag Ctr, Harbin 150081, Peoples R China

Recommended Citation:
Liu, Ji,Guo, Ji-Tao,Li, Yong-Guo,et al. The type VI secretion system gene cluster of Salmonella typhimurium: Required for full virulence in mice[J]. JOURNAL OF BASIC MICROBIOLOGY,2013,53(7):600-607.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Ji]'s Articles
[Guo, Ji-Tao]'s Articles
[Li, Yong-Guo]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Ji]‘s Articles
[Guo, Ji-Tao]‘s Articles
[Li, Yong-Guo]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace