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学科主题: 药学
题名:
Qualitative evaluation and quantitative determination of 10 major active components in Carthamus tinctorius L. by high-performance liquid chromatography coupled with diode array detector
作者: Fan, Li; Zhao, Hai-Yu; Xu, Man; Zhou, Lei; Guo, Hui; Han, Jian; Wang, Bao-Rong; Guo, De-An
关键词: HPLC-DAD ; Carthamus tinctorius L. ; Determination ; Quality control ; 6-Hydroxyapigenin ; 6-O-glucoside-7-O-glucuronide
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-03-13
DOI: 10.1016/j.chroma.2008.03.046
卷: 1216, 期:11, 页:2063-2070
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: OIL CAKE ; SAFFLOWER YELLOW ; FLAVONOIDS
英文摘要:

Flavonoids in the water extract of Carthamus tinctorius L. exhibit potent biological activities such as anti-coagulant, vasodilation, anti-oxidant, neuroprotection and immunosuppresant. A high-performance liquid chromatographic method was established to evaluate the quality of Carthamus tinctorius through a simultaneous quantitation of eight flavonoids, hydroxysafflor yellow A (2), 6-hydroxykaempferol 3,6-di-O-beta-glucoside-7-O-beta-glucuronide (3), 6-hydroxykaempferol 3,6,7-tri-O-beta-glucoside (4), 6-hydroxykaempferol 3-O-beta-rutinoside-6-O-beta-glucoside (6), 6-hydroxykaempferol 3,6-di-O-beta-glucoside (7). 6-hydroxyapigenin 6-0-glucoside-7-0-glucuronide (8), anhydrosafflor yellow B (9), and kaempferol 3-0-beta-rutinoside (10), together with two other Compounds named guanosine (1) and syringin (5). Among them, compound 8 was identified as a new Compound. The Compounds were separated oil an Alltech Alltima-C(18) column with gradient elution of acetonitrile and 0.01% trifluoroacetic acid. The detection wavelength was 280 nm. All the Compounds showed good linearity (r(2) >= 0.9989). The recoveries, Measured at three concentration levels, varied from 94.9% to 105.2%. This method was also validated with respect to precision, repeatability and accuracy, and was successfully applied to quantify the 10 components in 46 batches of C tinctorius samples from different areas. Significant variations were found in the contents of these compounds in these samples. Compared with the reported analytical methods of C. tinctorius, this simple and reliable method provided a new basis for overall assessment on quality Of C. tinctorius and Should be considered as a suitable quality control method. (C) 2008 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-063-112 ; 104218
项目资助者: Changjiang Scholar and Innovative Research Team in University ; Cultivation Fund of the Key Scientific and Technical Innovation Project ; Ministry of Education of China
WOS记录号: WOS:000264226500011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67876
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 10083, Peoples R China

Recommended Citation:
Fan, Li,Zhao, Hai-Yu,Xu, Man,et al. Qualitative evaluation and quantitative determination of 10 major active components in Carthamus tinctorius L. by high-performance liquid chromatography coupled with diode array detector[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2063-2070.
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