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Qualitative evaluation and quantitative determination of 10 major active components in Carthamus tinctorius L. by high-performance liquid chromatography coupled with diode array detector
Fan, Li; Zhao, Hai-Yu; Xu, Man; Zhou, Lei; Guo, Hui; Han, Jian; Wang, Bao-Rong; Guo, De-An
关键词HPLC-DAD Carthamus tinctorius L. Determination Quality control 6-Hydroxyapigenin 6-O-glucoside-7-O-glucuronide
刊名JOURNAL OF CHROMATOGRAPHY A
2009-03-13
DOI10.1016/j.chroma.2008.03.046
1216期:11页:2063-2070
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]OIL CAKE ; SAFFLOWER YELLOW ; FLAVONOIDS
英文摘要

Flavonoids in the water extract of Carthamus tinctorius L. exhibit potent biological activities such as anti-coagulant, vasodilation, anti-oxidant, neuroprotection and immunosuppresant. A high-performance liquid chromatographic method was established to evaluate the quality of Carthamus tinctorius through a simultaneous quantitation of eight flavonoids, hydroxysafflor yellow A (2), 6-hydroxykaempferol 3,6-di-O-beta-glucoside-7-O-beta-glucuronide (3), 6-hydroxykaempferol 3,6,7-tri-O-beta-glucoside (4), 6-hydroxykaempferol 3-O-beta-rutinoside-6-O-beta-glucoside (6), 6-hydroxykaempferol 3,6-di-O-beta-glucoside (7). 6-hydroxyapigenin 6-0-glucoside-7-0-glucuronide (8), anhydrosafflor yellow B (9), and kaempferol 3-0-beta-rutinoside (10), together with two other Compounds named guanosine (1) and syringin (5). Among them, compound 8 was identified as a new Compound. The Compounds were separated oil an Alltech Alltima-C(18) column with gradient elution of acetonitrile and 0.01% trifluoroacetic acid. The detection wavelength was 280 nm. All the Compounds showed good linearity (r(2) >= 0.9989). The recoveries, Measured at three concentration levels, varied from 94.9% to 105.2%. This method was also validated with respect to precision, repeatability and accuracy, and was successfully applied to quantify the 10 components in 46 batches of C tinctorius samples from different areas. Significant variations were found in the contents of these compounds in these samples. Compared with the reported analytical methods of C. tinctorius, this simple and reliable method provided a new basis for overall assessment on quality Of C. tinctorius and Should be considered as a suitable quality control method. (C) 2008 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000264226500011
引用统计
被引频次:83[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67876
专题北京大学药学院
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 10083, Peoples R China
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GB/T 7714
Fan, Li,Zhao, Hai-Yu,Xu, Man,et al. Qualitative evaluation and quantitative determination of 10 major active components in Carthamus tinctorius L. by high-performance liquid chromatography coupled with diode array detector[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2063-2070.
APA Fan, Li.,Zhao, Hai-Yu.,Xu, Man.,Zhou, Lei.,Guo, Hui.,...&Guo, De-An.(2009).Qualitative evaluation and quantitative determination of 10 major active components in Carthamus tinctorius L. by high-performance liquid chromatography coupled with diode array detector.JOURNAL OF CHROMATOGRAPHY A,1216(11),2063-2070.
MLA Fan, Li,et al."Qualitative evaluation and quantitative determination of 10 major active components in Carthamus tinctorius L. by high-performance liquid chromatography coupled with diode array detector".JOURNAL OF CHROMATOGRAPHY A 1216.11(2009):2063-2070.
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