IR@PKUHSC  > 北京大学临床肿瘤学院  > 细胞生物学研究室
学科主题临床医学
Prokaryotic expression of PEX: a C-terminal fragment of matrix metalloproteinase 2 and its effect on the inhibition of angiogenesis
Li, JP; Zhang, GM; Ke, Y; Lin, BY; Zhao, W; Hu, Y; Ning, T; Lin, ZX; Zhang, ZQ
关键词MMP-2 PEX calmodulin-binding peptide fusion protein angiogenesis
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2002-02-01
29期:1页:120-123
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]INTEGRIN ALPHA(V)BETA(3) ; CELLS ; SURFACE ; GROWTH
英文摘要

To express chick MMP-2 C-terminal hemopexin-like fragment in E. coli and to explore whether it has the biological effect on the inhibition of angiogenesis, a 630 bp of MMP-2 C-terminal coding fragment (PEX) was cloned into prokaryotic expression vector pCal-n from primary cultured day 10 chicken embryo fibroblasts using RT-PCR. The relatively pure inclusion bodies of CBP/PEX fusion protein were denatured and renatured by guandine.HCl method from PHX/pCal-n plasmid transformed log phase E. coli BL21 (Des) pLys induced with IPTG. Growth curve of human umbilical vein endothelial cells (HUVEC) and chick embryo chorioallantoic membrane (CAM) assay were performed to examine the effects on the inhibition of angiogenesis. The prokaryotic expressed CBP/PEX fusion protein has the biological activity and can inhibit the growth of HUVEC in vitro and the angiogenesis on chick CAM in vivo. These results suggest that the expressed PEX is to be a novel potential therapeutic inhibitor of diseases associated with angiogenesis.

语种中文
WOS记录号WOS:000174144100028
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67888
专题北京大学临床肿瘤学院_细胞生物学研究室
作者单位1.Xinxiang Med Univ, Xinxiang 453000, Peoples R China
2.Beijing Univ, Sch Oncol, Dept Cell Biol, Beijing 100034, Peoples R China
3.Beijing Univ, Beijing Inst Canc Res, Beijing 100034, Peoples R China
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GB/T 7714
Li, JP,Zhang, GM,Ke, Y,et al. Prokaryotic expression of PEX: a C-terminal fragment of matrix metalloproteinase 2 and its effect on the inhibition of angiogenesis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2002,29(1):120-123.
APA Li, JP.,Zhang, GM.,Ke, Y.,Lin, BY.,Zhao, W.,...&Zhang, ZQ.(2002).Prokaryotic expression of PEX: a C-terminal fragment of matrix metalloproteinase 2 and its effect on the inhibition of angiogenesis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,29(1),120-123.
MLA Li, JP,et al."Prokaryotic expression of PEX: a C-terminal fragment of matrix metalloproteinase 2 and its effect on the inhibition of angiogenesis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 29.1(2002):120-123.
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