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学科主题临床医学
Xingshentongqiao Decoction Mediates Proliferation, Apoptosis, Orexin-A Receptor and Orexin-B Receptor Messenger Ribonucleic Acid Expression and Represses Mitogen-activated Protein Kinase Signaling
Dong, Yuanli1; Li, Mei2; Wang, Shaojie1; Dong, Yuwei2; Zhao, Hongxia3; Dai, Zhong1
关键词Hypocretin Signaling Mitogen-activated Protein Kinases Narcolepsy Traditional Chinese Medicine Xingshentongqiao Decoction
刊名CHINESE MEDICAL JOURNAL
2015-01-05
DOI10.4103/0366-6999.147826
128期:1页:98-104
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]HYPOTHALAMIC NEUROPEPTIDES ; NEUROBLASTOMA-CELLS ; ENERGY HOMEOSTASIS ; FEEDING-BEHAVIOR ; REGULATED-KINASE ; NARCOLEPSY ; MECHANISMS ; CATAPLEXY ; NEURONS ; PATHWAY
英文摘要

Background: Hypocretin (HCRT) signaling plays an important role in the pathogenesis of narcolepsy and can be significantly influenced by Chinese herbal therapy. Our previous study showed that xingshentongqiao decoction (XSTQ) is clinically effective for the treatment of narcolepsy. To determine whether XSTQ improves narcolepsy by modulating HCRT signaling, we investigated its effects on SH-SY5Y cell proliferation, apoptosis, and HCRT receptor 1/2 (orexin receptor 1 [OX1R] and orexin receptor 2 [OX2R]) expression. The signaling pathways involved in these processes were also assessed.

Methods: The effects of XSTQ on proliferation and apoptosis in SH-SY5Y cells were assessed using cell counting kit-8 and annexin V-fluorescein isothiocyanate assays. OX1R and OX2R expression was assessed by quantitative real-time polymerase chain reaction analysis. Western blotting for mitogen-activated protein kinase (MAPK) pathway activation was performed to further assess the signaling mechanism of XSTQ.

Results: XSTQ reduced the proliferation and induced apoptosis of SH-SY5Y cells. This effect was accompanied by the upregulation of OX1R and OX2R expression and the reduced phosphorylation of extracellular signal-regulated kinase (Erk) 1/2, p 3 8 MAPK and c-Jun N-terminal kinase (JNK).

Conclusions: XSTQ inhibits proliferation and induces apoptosis in SH-SY5Y cells. XSTQ also promotes OX1R and OX2R expression. These effects are associated with the repression of the Erkl/2, p38 MAPK, and JNK signaling pathways. These results define a molecular mechanism for XSTQ in regulating HCRT and MAPK activation, which may explain its ability to treat narcolepsy.

语种英语
WOS记录号WOS:000349521500017
项目编号81273712 ; RDC 2012-22
资助机构National Natural Science Foundation of China ; Research and Development Foundation of Peking University People&prime ; s Hospital
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67892
专题北京大学第二临床医学院_中医科
北京大学第一临床医学院_眼科
作者单位1.Hosp Mentougou, Dept Tradit Chinese Med, Beijing 102300, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Tradit Chinese Med, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Inst Clin Mol Biol, Beijing 100044, Peoples R China
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GB/T 7714
Dong, Yuanli,Li, Mei,Wang, Shaojie,et al. Xingshentongqiao Decoction Mediates Proliferation, Apoptosis, Orexin-A Receptor and Orexin-B Receptor Messenger Ribonucleic Acid Expression and Represses Mitogen-activated Protein Kinase Signaling[J]. CHINESE MEDICAL JOURNAL,2015,128(1):98-104.
APA Dong, Yuanli,Li, Mei,Wang, Shaojie,Dong, Yuwei,Zhao, Hongxia,&Dai, Zhong.(2015).Xingshentongqiao Decoction Mediates Proliferation, Apoptosis, Orexin-A Receptor and Orexin-B Receptor Messenger Ribonucleic Acid Expression and Represses Mitogen-activated Protein Kinase Signaling.CHINESE MEDICAL JOURNAL,128(1),98-104.
MLA Dong, Yuanli,et al."Xingshentongqiao Decoction Mediates Proliferation, Apoptosis, Orexin-A Receptor and Orexin-B Receptor Messenger Ribonucleic Acid Expression and Represses Mitogen-activated Protein Kinase Signaling".CHINESE MEDICAL JOURNAL 128.1(2015):98-104.
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