北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Expression of the human fast-twitch skeletal muscle troponin I cDNA in a human ovarian carcinoma suppresses tumor growth
作者: Xiong GuangWu; Yang Li; Wei YuQuan; Wang ShiLang; Tian Ling; Lei Song; Kan Bing; Mao YongQiu
关键词: angiogenesis ; gene therapy ; Tnl-fast ; ovarian carcinoma
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2007-02-01
DOI: 10.1007/s11427-007-2032-7
卷: 50, 期:1, 页:93-100
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: ANGIOGENESIS INHIBITOR ; MURINE MELANOMA ; CANCER ; PROLIFERATION ; APOPTOSIS ; CELLS ; FIBROSARCOMA ; ANGIOSTATIN ; MECHANISMS ; METASTASES
英文摘要:

To explore the efficiency and mechanism of ovarian carcinoma gene therapy with the human fast-twitch skeletal muscle troponin I gene (Tnl-fast), Tnl-fast cDNA was transferred into human ovarian adenocarcinoma cell-line SK-OV-3. In vitro, the cell growth and cell cycle of Tnl-fast-, vector-, and mock-transfected cells were determined by MTT and flow cytometry assay, respectively. The conditioned media of Tnl-fast-, vector-, and mock-transfected SK-OV-3 cells were collected, and the cell proliferation inhibiting rates of human umbilical cord venous endothelial cells (HUVECs) by the three conditioned media were assayed. All the three cell lines were implanted into node mice, and the tumor growth, cell apoptosis, angiogenesis, and expression of Tnl-fast were observed or analyzed, respectively. In vitro, expression of Tnl-fast protein had no inhibiting effect on the growth of the dominant and stable transfectant cells, but endothelium, when compared with vector-transfected cells and nontransfected parental SK-OV-3 cells. Implantation of stable clone expressing Tnl-fast in the female BALB/c nude mice inhibits primary tumor growth by an average of 73%. The nude mice grafts expressing Tnl-fast exhibit a significant decrease of microvascular density, a higher rate of tumor cells apoptosis and a comparable proliferation rate as control. Our study, to our knowledge, shows the slowed down growth of the primary ovarian carcinoma, suggested that grafts were self-inhibitory by halting angiogenesis. Our data might also provide a novel useful strategy for cancer therapy by antiangiogenic gene therapy with a specific angiogenesis inhibitor Tnl-fast.

语种: 英语
WOS记录号: WOS:000245146700014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67942
Appears in Collections:北京大学第三临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Sichuan Univ, W China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China
2.Sichuan Univ, Huaxi Hosp 2, Dept Obstet & Gynecol, Chengdu 610041, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100083, Peoples R China

Recommended Citation:
Xiong GuangWu,Yang Li,Wei YuQuan,et al. Expression of the human fast-twitch skeletal muscle troponin I cDNA in a human ovarian carcinoma suppresses tumor growth[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2007,50(1):93-100.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xiong GuangWu]'s Articles
[Yang Li]'s Articles
[Wei YuQuan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xiong GuangWu]‘s Articles
[Yang Li]‘s Articles
[Wei YuQuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace