IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Expression of the human fast-twitch skeletal muscle troponin I cDNA in a human ovarian carcinoma suppresses tumor growth
Xiong GuangWu; Yang Li; Wei YuQuan; Wang ShiLang; Tian Ling; Lei Song; Kan Bing; Mao YongQiu
关键词angiogenesis gene therapy Tnl-fast ovarian carcinoma
刊名SCIENCE IN CHINA SERIES C-LIFE SCIENCES
2007-02-01
DOI10.1007/s11427-007-2032-7
50期:1页:93-100
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]ANGIOGENESIS INHIBITOR ; MURINE MELANOMA ; CANCER ; PROLIFERATION ; APOPTOSIS ; CELLS ; FIBROSARCOMA ; ANGIOSTATIN ; MECHANISMS ; METASTASES
英文摘要

To explore the efficiency and mechanism of ovarian carcinoma gene therapy with the human fast-twitch skeletal muscle troponin I gene (Tnl-fast), Tnl-fast cDNA was transferred into human ovarian adenocarcinoma cell-line SK-OV-3. In vitro, the cell growth and cell cycle of Tnl-fast-, vector-, and mock-transfected cells were determined by MTT and flow cytometry assay, respectively. The conditioned media of Tnl-fast-, vector-, and mock-transfected SK-OV-3 cells were collected, and the cell proliferation inhibiting rates of human umbilical cord venous endothelial cells (HUVECs) by the three conditioned media were assayed. All the three cell lines were implanted into node mice, and the tumor growth, cell apoptosis, angiogenesis, and expression of Tnl-fast were observed or analyzed, respectively. In vitro, expression of Tnl-fast protein had no inhibiting effect on the growth of the dominant and stable transfectant cells, but endothelium, when compared with vector-transfected cells and nontransfected parental SK-OV-3 cells. Implantation of stable clone expressing Tnl-fast in the female BALB/c nude mice inhibits primary tumor growth by an average of 73%. The nude mice grafts expressing Tnl-fast exhibit a significant decrease of microvascular density, a higher rate of tumor cells apoptosis and a comparable proliferation rate as control. Our study, to our knowledge, shows the slowed down growth of the primary ovarian carcinoma, suggested that grafts were self-inhibitory by halting angiogenesis. Our data might also provide a novel useful strategy for cancer therapy by antiangiogenic gene therapy with a specific angiogenesis inhibitor Tnl-fast.

语种英语
WOS记录号WOS:000245146700014
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67942
专题北京大学第三临床医学院
作者单位1.Sichuan Univ, W China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China
2.Sichuan Univ, Huaxi Hosp 2, Dept Obstet & Gynecol, Chengdu 610041, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Xiong GuangWu,Yang Li,Wei YuQuan,et al. Expression of the human fast-twitch skeletal muscle troponin I cDNA in a human ovarian carcinoma suppresses tumor growth[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2007,50(1):93-100.
APA Xiong GuangWu.,Yang Li.,Wei YuQuan.,Wang ShiLang.,Tian Ling.,...&Mao YongQiu.(2007).Expression of the human fast-twitch skeletal muscle troponin I cDNA in a human ovarian carcinoma suppresses tumor growth.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,50(1),93-100.
MLA Xiong GuangWu,et al."Expression of the human fast-twitch skeletal muscle troponin I cDNA in a human ovarian carcinoma suppresses tumor growth".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 50.1(2007):93-100.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xiong GuangWu]的文章
[Yang Li]的文章
[Wei YuQuan]的文章
百度学术
百度学术中相似的文章
[Xiong GuangWu]的文章
[Yang Li]的文章
[Wei YuQuan]的文章
必应学术
必应学术中相似的文章
[Xiong GuangWu]的文章
[Yang Li]的文章
[Wei YuQuan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。