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学科主题基础医学
Transposon integration enhances expression of stress response genes
Feng, Gang1,2; Leem, Young-Eun1; Levin, Henry L.1
刊名NUCLEIC ACIDS RESEARCH
2013
DOI10.1093/nar/gks1185
41期:2页:775-789
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]TRANSFER-RNA GENES ; POL-II PROMOTERS ; SCHIZOSACCHAROMYCES-POMBE ; FISSION YEAST ; SACCHAROMYCES-CEREVISIAE ; INSERTIONAL MUTAGENESIS ; RETROTRANSPOSON TY1 ; LTR-RETROTRANSPOSON ; MAMMARY-CANCER ; TRANSCRIPTION
英文摘要

Transposable elements possess specific patterns of integration. The biological impact of these integration profiles is not well understood. Tf1, a long-terminal repeat retrotransposon in Schizosaccharomyces pombe, integrates into promoters with a preference for the promoters of stress response genes. To determine the biological significance of Tf1 integration, we took advantage of saturated maps of insertion activity and studied how integration at hot spots affected the expression of the adjacent genes. Our study revealed that Tf1 integration did not reduce gene expression. Importantly, the insertions activated the expression of 6 of 32 genes tested. We found that Tf1 increased gene expression by inserting enhancer activity. Interestingly, the enhancer activity of Tf1 could be limited by Abp1, a host surveillance factor that sequesters transposon sequences into structures containing histone deacetylases. We found the Tf1 promoter was activated by heat treatment and, remarkably, only genes that themselves were induced by heat could be activated by Tf1 integration, suggesting a synergy of Tf1 enhancer sequence with the stress response elements of target promoters. We propose that the integration preference of Tf1 for the promoters of stress response genes and the ability of Tf1 to enhance the expression of these genes co-evolved to promote the survival of cells under stress.

语种英语
WOS记录号WOS:000314121100017
资助机构Intramural Research Program of the National Institutes of Health from the Eunice Kennedy Shriver National Institute of Child Health and Human Development ; NIH intramural program ; NICHD
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被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67946
专题北京大学基础医学院
作者单位1.Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Eukaryot Transposable Elements, Program Cellular Regulat & Metab, NIH, Bethesda, MD 20892 USA
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China
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Feng, Gang,Leem, Young-Eun,Levin, Henry L.. Transposon integration enhances expression of stress response genes[J]. NUCLEIC ACIDS RESEARCH,2013,41(2):775-789.
APA Feng, Gang,Leem, Young-Eun,&Levin, Henry L..(2013).Transposon integration enhances expression of stress response genes.NUCLEIC ACIDS RESEARCH,41(2),775-789.
MLA Feng, Gang,et al."Transposon integration enhances expression of stress response genes".NUCLEIC ACIDS RESEARCH 41.2(2013):775-789.
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