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学科主题: 基础医学
题名:
Transposon integration enhances expression of stress response genes
作者: Feng, Gang1,2; Leem, Young-Eun1; Levin, Henry L.1
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2013
DOI: 10.1093/nar/gks1185
卷: 41, 期:2, 页:775-789
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: TRANSFER-RNA GENES ; POL-II PROMOTERS ; SCHIZOSACCHAROMYCES-POMBE ; FISSION YEAST ; SACCHAROMYCES-CEREVISIAE ; INSERTIONAL MUTAGENESIS ; RETROTRANSPOSON TY1 ; LTR-RETROTRANSPOSON ; MAMMARY-CANCER ; TRANSCRIPTION
英文摘要:

Transposable elements possess specific patterns of integration. The biological impact of these integration profiles is not well understood. Tf1, a long-terminal repeat retrotransposon in Schizosaccharomyces pombe, integrates into promoters with a preference for the promoters of stress response genes. To determine the biological significance of Tf1 integration, we took advantage of saturated maps of insertion activity and studied how integration at hot spots affected the expression of the adjacent genes. Our study revealed that Tf1 integration did not reduce gene expression. Importantly, the insertions activated the expression of 6 of 32 genes tested. We found that Tf1 increased gene expression by inserting enhancer activity. Interestingly, the enhancer activity of Tf1 could be limited by Abp1, a host surveillance factor that sequesters transposon sequences into structures containing histone deacetylases. We found the Tf1 promoter was activated by heat treatment and, remarkably, only genes that themselves were induced by heat could be activated by Tf1 integration, suggesting a synergy of Tf1 enhancer sequence with the stress response elements of target promoters. We propose that the integration preference of Tf1 for the promoters of stress response genes and the ability of Tf1 to enhance the expression of these genes co-evolved to promote the survival of cells under stress.

语种: 英语
项目资助者: Intramural Research Program of the National Institutes of Health from the Eunice Kennedy Shriver National Institute of Child Health and Human Development ; NIH intramural program ; NICHD
WOS记录号: WOS:000314121100017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67946
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Eukaryot Transposable Elements, Program Cellular Regulat & Metab, NIH, Bethesda, MD 20892 USA
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Feng, Gang,Leem, Young-Eun,Levin, Henry L.. Transposon integration enhances expression of stress response genes[J]. NUCLEIC ACIDS RESEARCH,2013,41(2):775-789.
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