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学科主题: 药学
题名:
Neuroprotection by Manganese Superoxide Dismutase (MnSOD) Mimics: Antioxidant Effect and Oxidative Stress Regulation in Acute Experimental Stroke
作者: Huang, Hai-Feng; Guo, Fei; Cao, Yuan-Zhao; Shi, Wen; Xia, Qing
关键词: Ischemic stroke ; Manganese superoxide dismutase ; Middle cerebral artery occlusion ; Neuroprotection
刊名: CNS NEUROSCIENCE & THERAPEUTICS
发表日期: 2012-10-01
DOI: 10.1111/j.1755-5949.2012.00380.x
卷: 18, 期:10, 页:811-818
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences ; Pharmacology & Pharmacy
研究领域[WOS]: Neurosciences & Neurology ; Pharmacology & Pharmacy
关键词[WOS]: CEREBRAL-ARTERY OCCLUSION ; RAT CORTICAL-NEURONS ; ENDOPLASMIC-RETICULUM ; IN-VIVO ; MITOCHONDRIAL-FUNCTION ; HYDROGEN-PEROXIDE ; PROTECTS NEURONS ; ISCHEMIC-STROKE ; INJURY ; BRAIN
英文摘要:

Aims Manganese superoxide dismutase (MnSOD), one of the most crucial antioxidant enzymes in the central nervous system, is thought to be one of the major mechanisms by which cells counteract the injuries of reactive oxygen species after cerebral ischemia. In this study, we used a novel synthesized compound (MnTm4PyP) with highly effective superoxide dismutase activity to study the therapeutic potential of MnSOD and the possible underlying mechanisms in cerebral ischemia. Methods Primary cultured cortical neurons were used to examine the protective effect of the compounds. Mice with middle cerebral artery occlusion were used as ischemic stroke animal model. Animals were pretreated with MnTm4PyP intravenously 30 similar to min before surgery. At 24 similar to h after surgery, neurological behavior and histological function were observed. Infarcted cortex tissues and cultured neurons were collected for investigation of the oxidative stress signaling pathways. Results In vitro studies revealed that MnSOD mimic MnTm4PyP pretreatment significantly increased viability of neurons after injury by H2O2. Intracellular superoxide radical levels were eliminated. In vivo experiments demonstrated MnTm4PyP pretreatment reduced infarct volume and improved neurological function. The MnSOD mimic alleviated oxidative stress and apoptosis. Conclusion MnSOD is an effective therapeutic target in ischemic stroke prevention because of its antioxidant effects and oxidative stress regulation.

语种: 英语
所属项目编号: 20901005 ; 2010010 ; 2010CB12300
项目资助者: National Natural Science Foundation of China ; Beijing Nova Program ; National Basic Research Program of China
WOS记录号: WOS:000309186000002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67948
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: Peking Univ, State Key Lab Nat & Biomimet Drugs, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Hai-Feng,Guo, Fei,Cao, Yuan-Zhao,et al. Neuroprotection by Manganese Superoxide Dismutase (MnSOD) Mimics: Antioxidant Effect and Oxidative Stress Regulation in Acute Experimental Stroke[J]. CNS NEUROSCIENCE & THERAPEUTICS,2012,18(10):811-818.
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