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学科主题: 临床医学
题名:
Development and Validation of a New HPV Genotyping Assay Based on Next-Generation Sequencing
作者: Yi, Xin1,2; Zou, Jing1; Xu, Jiajia2; Liu, Tao2; Liu, Tengfei1; Hua, Sang1; Xi, Feng1; Nie, Xifang2; Ye, Lili1; Luo, Yufen1; Xu, Lin1; Du, Hui3; Wu, Ruifang3; Yang, Ling2; Liu, Ren4; Yang, Bin5; Wang, Jian1; Belinson, Jerome L.6
关键词: Cervical cancer screening ; Self-sampling ; HPV genotyping test ; Next-generation sequencing (NGS) ; Ion Torrent PGM ; MiSeq
刊名: AMERICAN JOURNAL OF CLINICAL PATHOLOGY
发表日期: 2014-06-01
DOI: 10.1309/AJCP9P2KJSXEKCJB
卷: 141, 期:6, 页:796-804
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: HIGH-RISK HPV ; HUMAN-PAPILLOMAVIRUS TYPES ; MASS-SPECTROMETRY SYSTEM ; CERVICAL-CANCER ; CLINICAL-TRIAL ; PERFORMANCE ; PLATFORMS ; LESIONS ; DETECT ; CHINA
英文摘要:

Objectives: We developed a new human papillomavirus (HPV) genotyping assay based on multiplex polymerase chain reaction and next-generation sequencing (NGS) methods for large-scale cervical cancer screening.

Methods: We first trained the assay on 1,170 self-collected samples, balancing the cutoff points for high-risk types. Then using 4,262 separate self-collected specimens, we compared concordance, sensitivity, and specificity for cervical intraepithelial neoplasia type 2 (CIN2) or higher and CIN type 3 (CIN3) or higher of the HPV sequencing assay with that of Hybrid Capture 2 (HC2) direct samples and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry assay self-samples.

Results: All assays had a good agreement. The sensitivity for CIN2 or higher and CIN3 or higher of the self-sampling specimens tested with the sequencing assay run on both MiSeq and Ion Torrent Personal Genome Machine sequencer was similar to that of direct-sampling specimens tested with HC2 (P > .05), but the specificity of the sequencing assay for CIN2 or higher and CIN3 or higher was significantly higher than that of HC2 (P < .01).

Conclusions: This population-based study has demonstrated the applicability of a new NGS high-risk HPV assay for primary cervical cancer screening based on self-collection.

语种: 英语
项目资助者: Special Funds for Biotechnology Industry from Shenzhen Development and Reform Commission of China ; Funds for Shenzhen Clinical Molecular Diagnostic Engineering Laboratory from Shenzhen Development and Reform Commission of China ; Preventive Oncology International ; Hologic, Bedford, MA ; Qiagen, Gaithersburg, MD ; Gen-Probe, Bedford, MA ; Merck, Whitehouse Station, NJ ; GE Healthcare, Fairfield, CT
WOS记录号: WOS:000336239800007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67952
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.BGI Shenzhen, Shenzhen, Peoples R China
2.BGI Tianjin, Tianjin, Peoples R China
3.Peking Univ, Shenzhen Hosp, Shenzhen, Peoples R China
4.Shenzhen Second Peoples Hosp, Shenzhen, Peoples R China
5.Cleveland Clin, Dept Anat Pathol, Cleveland, OH 44106 USA
6.Cleveland Clin, Prevent Oncol Int & Womens Hlth Inst, Cleveland, OH 44106 USA

Recommended Citation:
Yi, Xin,Zou, Jing,Xu, Jiajia,et al. Development and Validation of a New HPV Genotyping Assay Based on Next-Generation Sequencing[J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY,2014,141(6):796-804.
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