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学科主题: 基础医学
题名:
Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells
作者: Ma, Yanling; Xu, Yongsheng; Xiao, Zhifeng; Yang, Wei; Zhang, Chun; Song, E.; Du, Yiqin; Li, Lingsong
关键词: mesenchymal stem cell ; cornea ; transplantation ; angiogenesis and inflammation
刊名: STEM CELLS
发表日期: 2006-02-01
DOI: 10.1634/stemcells.2005-0046
卷: 24, 期:2, 页:315-321
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]: AMNIOTIC MEMBRANE TRANSPLANTATION ; OCULAR SURFACE ; STROMAL CELLS ; EPITHELIAL-CELLS ; DIFFERENTIATE ; MICE ; DEFICIENCY
英文摘要:

To examine whether transplantation of human mesenchymal stem cells (MSCs) could reconstruct the corneal damage and also whether grafted MSCs could differentiate into corneal epithelial cells, we isolated MSCs from healthy donors. After growth and expansion on amniotic membrane, cells were transplanted into rat corneas 7 days after chemical burns. Reconstruction of the damaged cornea and the rat vision were measured once a week by slit lamp and by an optokinetic head-tracking instrument, respectively. Corneas were then cut out, fixed, and imbedded for immunofluorescent study of the expression of keratin 3 and keratin-pan as epithelial cell markers. Expression of CD45, interleukin 2, and metalloproteinase-2 was also investigated for inflammation and inflammation-related angiogenesis. The data showed that transplantation of MSCs, like limbal epithelial stem cells, successfully reconstructed damaged rat corneal surface. Interestingly, the therapeutic effect of the transplantation may be associated with the inhibition of inflammation and angiogenesis after transplantation of MSCs rather than the epithelial differentiation from MSCs. This study provides the first line of evidence that MSCs can be used for reconstruction of damaged corneas, presenting a new source for autotransplantation in the treatment of corneal disorders.

语种: 英语
WOS记录号: WOS:000240635900013
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67956
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Ophthalmol, Hosp 3, Beijing 100083, Peoples R China

Recommended Citation:
Ma, Yanling,Xu, Yongsheng,Xiao, Zhifeng,et al. Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells[J]. STEM CELLS,2006,24(2):315-321.
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