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学科主题口腔医学
Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells
Liu, Yang; Han, Dong; Wang, Lei; Feng, Hailan
关键词Wnt10a Tooth development Cell proliferation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2013-05-17
DOI10.1016/j.bbrc.2013.03.088
434期:4页:717-721
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
资助者National Natural Science Foundation of China ; Construction Project for State Key Clinical Discipline ; National Natural Science Foundation of China ; Construction Project for State Key Clinical Discipline
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ODONTOBLAST DIFFERENTIATION ; ECTODERMAL DYSPLASIA ; TOOTH MORPHOGENESIS ; MAMMALIAN TOOTH ; EXPRESSION ; MOUSE
英文摘要

The WNT10a mutation has been found in patients with abnormal odontogenesis. In mice, Wnt10a expression is found in the tooth germ, but its role has not yet been elucidated. We aimed to investigate the role of Wnt10a in odontogenesis. Mesenchymal cells of the first mandibular molar germ at the bell stage were isolated, transfected with Wnt10a SiRNA or plasmid, and reassociated with epithelial part of the molar germ. Scrambled SiRNA or empty vector was used in the control group. The reassociated tooth germs were transplanted into mice subrenal capsules. After gene modification, dental mesenchymal cells cultured in vitro were checked for cell proliferation and the expression of Dspp was examined. All 12 reassociated tooth germs in the control group resumed odontogenesis, while only 5 of 12 in the Wnt10a knockdown group developed into teeth. After Wnt10a knockdown, the mesenchymal cells cultured in vitro presented repressed proliferation. Wnt10a knockdown and overexpression led to both down- and up-regulation of Dspp. We conclude that the down-regulation of Wnt10n impairs odontogensis and cell proliferation, and that Wnt10a regulates Dspp expression in mesenchymal cells. These findings help to elucidate the mechanism of abnormal tooth development in patients with the WNT10A mutation. (c) 2013 Elsevier Inc. All rights reserved.

语种英语
所属项目编号81271121
资助者National Natural Science Foundation of China ; Construction Project for State Key Clinical Discipline ; National Natural Science Foundation of China ; Construction Project for State Key Clinical Discipline
WOS记录号WOS:000320213200004
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67957
专题北京大学口腔医学院_口腔修复科
作者单位Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
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GB/T 7714
Liu, Yang,Han, Dong,Wang, Lei,et al. Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,434(4):717-721.
APA Liu, Yang,Han, Dong,Wang, Lei,&Feng, Hailan.(2013).Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,434(4),717-721.
MLA Liu, Yang,et al."Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 434.4(2013):717-721.
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