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学科主题: 口腔医学
题名:
Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells
作者: Liu, Yang; Han, Dong; Wang, Lei; Feng, Hailan
关键词: Wnt10a ; Tooth development ; Cell proliferation
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2013-05-17
DOI: 10.1016/j.bbrc.2013.03.088
卷: 434, 期:4, 页:717-721
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ODONTOBLAST DIFFERENTIATION ; ECTODERMAL DYSPLASIA ; TOOTH MORPHOGENESIS ; MAMMALIAN TOOTH ; EXPRESSION ; MOUSE
英文摘要:

The WNT10a mutation has been found in patients with abnormal odontogenesis. In mice, Wnt10a expression is found in the tooth germ, but its role has not yet been elucidated. We aimed to investigate the role of Wnt10a in odontogenesis. Mesenchymal cells of the first mandibular molar germ at the bell stage were isolated, transfected with Wnt10a SiRNA or plasmid, and reassociated with epithelial part of the molar germ. Scrambled SiRNA or empty vector was used in the control group. The reassociated tooth germs were transplanted into mice subrenal capsules. After gene modification, dental mesenchymal cells cultured in vitro were checked for cell proliferation and the expression of Dspp was examined. All 12 reassociated tooth germs in the control group resumed odontogenesis, while only 5 of 12 in the Wnt10a knockdown group developed into teeth. After Wnt10a knockdown, the mesenchymal cells cultured in vitro presented repressed proliferation. Wnt10a knockdown and overexpression led to both down- and up-regulation of Dspp. We conclude that the down-regulation of Wnt10n impairs odontogensis and cell proliferation, and that Wnt10a regulates Dspp expression in mesenchymal cells. These findings help to elucidate the mechanism of abnormal tooth development in patients with the WNT10A mutation. (c) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 81271121
项目资助者: National Natural Science Foundation of China ; Construction Project for State Key Clinical Discipline
WOS记录号: WOS:000320213200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67957
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

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作者单位: Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China

Recommended Citation:
Liu, Yang,Han, Dong,Wang, Lei,et al. Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,434(4):717-721.
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