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学科主题: 基础医学
题名:
Effects of Gekko Sulfated Polysaccharide-Protein Complex on the Defective Biorheological Characters of Dendritic Cells Under Tumor Microenvironment
作者: Chen, Dan1,2; Zhang, Xiaolan3; Du, Yuan4; Jia, Binbin5; Ka, Weibo1; Sun, Dagong1; Yao, Weijuan1; Wen, Zongyao1
关键词: Gekko swinhonis Guenther ; Sulfated polysaccharide ; Biorheology ; Dendritic cells ; Hepatocellular carcinoma
刊名: CELL BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2012
DOI: 10.1007/s12013-011-9282-5
卷: 62, 期:1, 页:193-201
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: T-CELLS ; GROWTH-FACTOR ; LYMPH-NODES ; CYTOSKELETON ; MATURATION ; CANCER ; RESPONSES ; MELANOMA ; LINE ; DIFFERENTIATION
英文摘要:

We previously isolated a sulfated polysaccharide-protein complex from Gekko swinhonis Guenther, a traditional Chinese medicine, and have demonstrated its direct anti-cancer effect on human hepatocellular carcinoma cell line SMMC-7721. Here we investigated the effects of Gekko sulfated polysaccharide-protein complex (GSPP) on the defective biorheological characters of dendritic cells (DCs) under SMMC-7721 microenvironment. Our findings have shown that the biorheological properties of DCs were severely impaired by SMMC-7721 microenvironment, including decreased cell deformability, migration, and electrophoresis mobility, increased osmotic fragilities, and changed organizations of cytoskeletal proteins. We also found decreased secretion of interleukin (IL)-12 and increased secretion of IL-10 in DCs. However, supernatant collected from nonmalignant liver cells had no effect on these parameters. SMMC-7721 cells were treated with GSPP and the supernatant was used to culture DCs. We found that the defective biorheological parameters of DCs, except for osmotic fragility, were partially or completely improved. The secretion of IL-12 did not change as compared with that of DCs in SMMC-7721 microenvironment, but the secretion of IL-10 was resumed to the control level. Our results indicate that GSPP could partially restore the defective biorheological characteristics of DCs via modifying the tumor microenvironment and decreasing the secretion of IL-10 of DCs.

语种: 英语
所属项目编号: 10572007 ; 30770532
项目资助者: National Natural Science Foundations of China
WOS记录号: WOS:000299055700028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67959
Appears in Collections:基础医学院_北京大学血液流变中心_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Hemorheol Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Tianjin Med Univ, Dept Pharmacol, Sch Basic Med Sci, Tianjin 300070, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China
4.Peking Univ, Hosp 3, Dept Urol, Beijing 100191, Peoples R China
5.Chengde Med Coll, Chengde 067000, Hebei Province, Peoples R China

Recommended Citation:
Chen, Dan,Zhang, Xiaolan,Du, Yuan,et al. Effects of Gekko Sulfated Polysaccharide-Protein Complex on the Defective Biorheological Characters of Dendritic Cells Under Tumor Microenvironment[J]. CELL BIOCHEMISTRY AND BIOPHYSICS,2012,62(1):193-201.
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