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学科主题: 临床医学
题名:
Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo
作者: Zhang, XL; Yu, CL; XuShi; Zhang, C; Tang, TT; Dai, KR
关键词: gene therapy ; citosan ; chondrocyte ; osteoarthritis ; knee joint
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2006-03-03
DOI: 10.1016/j.bbrc.2005.12.171
卷: 341, 期:1, 页:202-208
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: RECEPTOR ANTAGONIST GENE ; TUMOR-NECROSIS-FACTOR ; ARTICULAR-CARTILAGE ; MATRIX DEGRADATION ; GUINEA-PIGS ; OSTEOARTHRITIS ; INTERLEUKIN-1 ; TRANSFECTION ; THERAPY ; DNA
英文摘要:

Chitosan vector system is expected to be useful for direct gene therapy for joint disease. This study first sought to confirm that foreign genes can be transferred to articular chondrocytes in primary culture. Next, chitosan-DNA nanoparticles containing IL-IRa or IL-10 gene were injected directly into the knee joint cavities of osteoarthritis rabbits to clarify the in vivo transfer availability of the chitosan vectors. Clear expression of IL-IRa was detected in the knee joint synovial fluid of the chitosan IL-1Ra-injected group. While no expression was detected in the chitosan IL-10-injected group, this demonstrates that the transfection efficiency of chitosan-DNA nanoparticles was closely related to the type of the gene product. A significant reduction was also noted in the severity of histologic cartilage lesions in the group that received the chitosan IL-1Ra injection. This avenue may therefore represent a promising future treatment for osteoarthritis. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000235313400030
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67968
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Inst Sports Med, Beijing 100083, Peoples R China
2.Shanghai Jiao Tong Univ, Inst Hlth Sci, Sch Med, Shanghai 200025, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Med, Dept Orthopaed, Peoples Hosp 9, Shanghai 200011, Peoples R China
5.Peking Univ, Human Dis Genom Res Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, XL,Yu, CL,XuShi,et al. Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,341(1):202-208.
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