IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo
Zhang, XL; Yu, CL; XuShi; Zhang, C; Tang, TT; Dai, KR
关键词gene therapy citosan chondrocyte osteoarthritis knee joint
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2006-03-03
DOI10.1016/j.bbrc.2005.12.171
341期:1页:202-208
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]RECEPTOR ANTAGONIST GENE ; TUMOR-NECROSIS-FACTOR ; ARTICULAR-CARTILAGE ; MATRIX DEGRADATION ; GUINEA-PIGS ; OSTEOARTHRITIS ; INTERLEUKIN-1 ; TRANSFECTION ; THERAPY ; DNA
英文摘要

Chitosan vector system is expected to be useful for direct gene therapy for joint disease. This study first sought to confirm that foreign genes can be transferred to articular chondrocytes in primary culture. Next, chitosan-DNA nanoparticles containing IL-IRa or IL-10 gene were injected directly into the knee joint cavities of osteoarthritis rabbits to clarify the in vivo transfer availability of the chitosan vectors. Clear expression of IL-IRa was detected in the knee joint synovial fluid of the chitosan IL-1Ra-injected group. While no expression was detected in the chitosan IL-10-injected group, this demonstrates that the transfection efficiency of chitosan-DNA nanoparticles was closely related to the type of the gene product. A significant reduction was also noted in the severity of histologic cartilage lesions in the group that received the chitosan IL-1Ra injection. This avenue may therefore represent a promising future treatment for osteoarthritis. (c) 2006 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000235313400030
引用统计
被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67968
专题北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Inst Sports Med, Beijing 100083, Peoples R China
2.Shanghai Jiao Tong Univ, Inst Hlth Sci, Sch Med, Shanghai 200025, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Med, Dept Orthopaed, Peoples Hosp 9, Shanghai 200011, Peoples R China
5.Peking Univ, Human Dis Genom Res Ctr, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhang, XL,Yu, CL,XuShi,et al. Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,341(1):202-208.
APA Zhang, XL,Yu, CL,XuShi,Zhang, C,Tang, TT,&Dai, KR.(2006).Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,341(1),202-208.
MLA Zhang, XL,et al."Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 341.1(2006):202-208.
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