IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心
学科主题临床医学
Impaired Oocyte Quality Induced by Dehydroepiandrosterone Is Partially Rescued by Metformin Treatment
Huang, Ying1,2,3; Yu, Yang1,2; Gao, Jiangman1,3; Li, Rong1,3; Zhang, Chunmei1,2; Zhao, Hongcui1,3; Zhao, Yue1,2; Qiao, Jie1,2,3
刊名PLOS ONE
2015-03-26
DOI10.1371/journal.pone.0122370
10期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]POLYCYSTIC-OVARY-SYNDROME ; IN-VITRO ; INSULIN-RESISTANCE ; OXIDATIVE STRESS ; COMPLEX-I ; DEVELOPMENTAL COMPETENCE ; RESPIRATORY-CHAIN ; MOUSE OOCYTES ; FEMALE RATS ; WOMEN
英文摘要

The present study evaluated the influence of hyperandrogenism on oocyte quality using a murine PCOS model induced by dehydroepiandrosterone (DHEA) and further explored the effect of metformin treatment. Female BALB/c mice were treated with a vehicle control or DHEA (6 mg/100 g body weight) or DHEA plus metformin (50 mg/100 g body weight) for 20 consecutive days. DHEA-induced mice resembled some characters of human PCOS, such as irregular sexual cycles and polycystic ovaries. After the model validation was completed, metaphase II (MII) oocytes were retrieved and subsequent analyses of oocyte quality were performed. DHEA-treated mice yielded fewer MII oocytes, which displayed decreased mtDNA copy number, ATP content, inner mitochondrial membrane potential, excessive oxidative stress and impaired embryo development competence compared with those in control mice. Metformin treatment partially attenuated those damages, as evidenced by the increased fertilization and blastocyst rate, ATP content, GSH concentration and GSH/GSSG ratio, and decreased reactive oxygen species levels. No significant difference in normal spindle assembly was observed among the three groups. During in vitro maturation (IVM), the periods of germinal vesicle breakdown (GVBD) and the first polar body (PB1) extrusion were extended and the maturation rate of GVBD oocytes was decreased in DHEA mice compared with controls. Metformin treatment decreased the time elapsed of GVBD while had no effect on PB1 extrusion. These results indicated that excessive androgen is detrimental to oocyte quality while metformin treatment is, directly or indirectly, beneficial for oocyte quality improvement.

语种英语
WOS记录号WOS:000356353700187
项目编号2014CB943203 ; 31230047 ; 31371521 ; 81300457
资助机构Ministry of Science and Technology of China ; National Natural Science Foundation of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67998
专题北京大学第三临床医学院_生殖医学中心
北京大学第二临床医学院_麻醉科
北京大学第三临床医学院_妇产科
作者单位1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Reprod Med Ctr, Beijing 100871, Peoples R China
2.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Huang, Ying,Yu, Yang,Gao, Jiangman,et al. Impaired Oocyte Quality Induced by Dehydroepiandrosterone Is Partially Rescued by Metformin Treatment[J]. PLOS ONE,2015,10(3).
APA Huang, Ying.,Yu, Yang.,Gao, Jiangman.,Li, Rong.,Zhang, Chunmei.,...&Qiao, Jie.(2015).Impaired Oocyte Quality Induced by Dehydroepiandrosterone Is Partially Rescued by Metformin Treatment.PLOS ONE,10(3).
MLA Huang, Ying,et al."Impaired Oocyte Quality Induced by Dehydroepiandrosterone Is Partially Rescued by Metformin Treatment".PLOS ONE 10.3(2015).
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