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学科主题: 临床医学
题名:
Impaired Oocyte Quality Induced by Dehydroepiandrosterone Is Partially Rescued by Metformin Treatment
作者: Huang, Ying1,2,3; Yu, Yang1,2; Gao, Jiangman1,3; Li, Rong1,3; Zhang, Chunmei1,2; Zhao, Hongcui1,3; Zhao, Yue1,2; Qiao, Jie1,2,3
刊名: PLOS ONE
发表日期: 2015-03-26
DOI: 10.1371/journal.pone.0122370
卷: 10, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: POLYCYSTIC-OVARY-SYNDROME ; IN-VITRO ; INSULIN-RESISTANCE ; OXIDATIVE STRESS ; COMPLEX-I ; DEVELOPMENTAL COMPETENCE ; RESPIRATORY-CHAIN ; MOUSE OOCYTES ; FEMALE RATS ; WOMEN
英文摘要:

The present study evaluated the influence of hyperandrogenism on oocyte quality using a murine PCOS model induced by dehydroepiandrosterone (DHEA) and further explored the effect of metformin treatment. Female BALB/c mice were treated with a vehicle control or DHEA (6 mg/100 g body weight) or DHEA plus metformin (50 mg/100 g body weight) for 20 consecutive days. DHEA-induced mice resembled some characters of human PCOS, such as irregular sexual cycles and polycystic ovaries. After the model validation was completed, metaphase II (MII) oocytes were retrieved and subsequent analyses of oocyte quality were performed. DHEA-treated mice yielded fewer MII oocytes, which displayed decreased mtDNA copy number, ATP content, inner mitochondrial membrane potential, excessive oxidative stress and impaired embryo development competence compared with those in control mice. Metformin treatment partially attenuated those damages, as evidenced by the increased fertilization and blastocyst rate, ATP content, GSH concentration and GSH/GSSG ratio, and decreased reactive oxygen species levels. No significant difference in normal spindle assembly was observed among the three groups. During in vitro maturation (IVM), the periods of germinal vesicle breakdown (GVBD) and the first polar body (PB1) extrusion were extended and the maturation rate of GVBD oocytes was decreased in DHEA mice compared with controls. Metformin treatment decreased the time elapsed of GVBD while had no effect on PB1 extrusion. These results indicated that excessive androgen is detrimental to oocyte quality while metformin treatment is, directly or indirectly, beneficial for oocyte quality improvement.

语种: 英语
所属项目编号: 2014CB943203 ; 31230047 ; 31371521 ; 81300457
项目资助者: Ministry of Science and Technology of China ; National Natural Science Foundation of China
WOS记录号: WOS:000356353700187
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67998
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Reprod Med Ctr, Beijing 100871, Peoples R China
2.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China

Recommended Citation:
Huang, Ying,Yu, Yang,Gao, Jiangman,et al. Impaired Oocyte Quality Induced by Dehydroepiandrosterone Is Partially Rescued by Metformin Treatment[J]. PLOS ONE,2015,10(3).
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