北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Gene cloning of secreted VEGF receptor flt-1 (I similar to IV extracellular domain) and the corresponding recombinant adenovirus construction and expression
作者: Zhang, H; Shou, CC
关键词: adenovirus ; flt-1 ; homogenous recombination ; expression
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2002-08-01
卷: 29, 期:4, 页:625-630
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: TRANSDUCTION
英文摘要:

The increasing data show that the soluble form of Flt-1 have the ability to block the biological activity of VEGF due to its high affinity to VEGF and formation of homologous. and heterogenous dimmer with transmembrane Flt-1 and KDR. The binding activity of Flt-1 focuses on its II and III extracellular domain. cDNA fragments encoding for signal peptide and flt-1 I similar to IV extracellular domain were amplified through RT-PCR with a pair of specific primers from human umbilical vein endothelial cells. Then, the sflt-1 gene was subcloned into pcDNA3-EF-1 vector, and the expression unit of EF-1-fit-1 was subcloned into shuttle vector of pAdTrack-CMV, linearized pAdTrack-EF-1-flt-1 was co-transformed into BJ5183 cells with adenoviral genomic plasmid of pAdEasy-1. The identified recombinant DNA was transfected into 293 cells to package adenovirus. From the supernatant and cell lysis, the presence of recombinant adenovirus was proved by PCR, and RT-PCR detection demonstrated the transcription of flt-1 in MGC803 cells infected with Ad-Track-EF-1-flt-1, and immunoprecipitation reveals that soluble Flt-1 can be secreted into the culture supernatant of infected MGC803 cells. These results indicated that tumor cells infected with the prepared recombinant adenovirus vector Ad-Track-EF-1-flt-1 can secrete soluble Flt-1, which will be further used for in vivo antiangiogenesis experiments.

语种: 中文
WOS记录号: WOS:000177560000026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68009
Appears in Collections:北京大学临床肿瘤学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, H,Shou, CC. Gene cloning of secreted VEGF receptor flt-1 (I similar to IV extracellular domain) and the corresponding recombinant adenovirus construction and expression[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2002,29(4):625-630.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, H]'s Articles
[Shou, CC]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, H]‘s Articles
[Shou, CC]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace