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学科主题: 临床医学
题名:
Ginsenoside-Rb1 Protects Hypoxic- and Ischemic-Damaged Cardiomyocytes by Regulating Expression of miRNAs
作者: Yan, Xu1,2,3; Xue, Jinrong4; Wu, Hongjin1; Wang, Shengqi5; Liu, Yuna3; Zheng, Sidao3; Zhang, Chengying3; Yang, Cui3
刊名: EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
发表日期: 2015
DOI: 10.1155/2015/171306
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Integrative & Complementary Medicine
研究领域[WOS]: Integrative & Complementary Medicine
关键词[WOS]: ACUTE MYOCARDIAL-INFARCTION ; OXIDATIVE STRESS ; RB1 ; INJURY ; REPERFUSION ; GINSENG ; MICE ; RAT ; APOPTOSIS ; PATHWAY
英文摘要:

Ginsenoside (GS-Rb1) is one of the most important active compounds of ginseng, with extensive evidence of its cardioprotective properties. However, the miRNA mediated mechanism of GS-Rb1 on cardiomyocytes remains unclear. Here, the roles of miRNAs in cardioprotective activity of GS-Rb1 were investigated in hypoxic-and ischemic-damaged cardiomyocytes. Neonatal rat cardiomyocytes (NRCMs) were first isolated, cultured, and then incubated with or without GS-Rb1 (2.5-40 mu M) in vitro under conditions of hypoxia and ischemia. Cell growth, proliferation, and apoptosis were detected by MTT and flow cytometry. Expressions of various microRNAs were analyzed by real-time PCR. Compared with that of the control group, GS-Rb1 significantly decreased cell death in a dose-dependent manner and expressions of mir-1, mir-29a, and mir-208 obviously increased in the experimental model groups. In contrast, expressions of mir-21 and mir-320 were significantly downregulated and GS-Rb1 could reverse the differences in a certain extent. The miRNAs might be involved in the protective effect of GS-Rb1 on the hypoxia/ischemia injuries in cardiomyocytes. The effect might be based on the upregulation of mir-1, mir-29a, and mir-208 and downregulation of mir-21 and mir-320. This might provide us a new target to explore the novel strategy for ischemic cardioprotection.

语种: 英语
所属项目编号: 81273890 ; 81173367 ; 2013ZZ-57
项目资助者: National Natural Science Foundation of China ; Postdoctoral Science Foundation of Beijing
WOS记录号: WOS:000355799900001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68015
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Haidian Sect, Beijing Haidian Hosp, Hosp 3, Beijing 100080, Peoples R China
2.Zhongguancun Haidian Sci Pk, Postdoctoral Workstn, Beijing 100195, Peoples R China
3.Beijing Hosp Integrated Tradit Chinese & Western, Beijing 100039, Peoples R China
4.Capital Med Univ, Beijing Anzhen Hosp, Dept Cardiovasc Surg, Beijing Aort Dis Ctr, Beijing 100029, Peoples R China
5.Beijing Inst Radiat Med, Beijing 100850, Peoples R China

Recommended Citation:
Yan, Xu,Xue, Jinrong,Wu, Hongjin,et al. Ginsenoside-Rb1 Protects Hypoxic- and Ischemic-Damaged Cardiomyocytes by Regulating Expression of miRNAs[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2015.
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