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学科主题: 临床医学
题名:
[Ca2+]i homeostasis and caspase-3 gene expression in verapamil -induced retinal pigment epithelium cells apoptosis in vitro
作者: Pang, Dong-Bo1; Hong, Jing2
关键词: [Ca2+]i homeostasis ; caspase-3 gene ; Ver-induced RPE cells apoptosis
刊名: INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
发表日期: 2010-12-18
卷: 3, 期:4, 页:288-290
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: MATRIX
英文摘要:

AIM: To study caspase-3 gene expression and [Ca2+]i homeostasis in verapamil (Ver)-induced human retinal pigment epithelium (RPE) cells apoptosis.

METHODS: Ver 80mg/L was applied in cultured human RPE cells for 12, 24 and 48 hours to induce RPE cells apoptosis. The expression of apoptotic effector gene caspase-3 was assessed by reverse transcription polymerase chain reaction (RT-PCR). Single cell was measured using fluorescence indicator Fura-3/AM with MetaFluo4.5/coolsnapfx/IX70 intracellular ca(2+) fluorescence imaging system.

RESULTS: High levels of expression of caspase-3 mRNA were observed in normal RPE cells and it significantly increased after co-cultured with Ver. The fluorescence in resting RPE cells was strong and distributed throughout the cells. The nucleus appeared more fluorescent than the cytoplasm. Calcium fluorescence of RPE cells attenuated after co-cultured with Ver.

CONCLUSION: Up-regulation of caspase-3 gene expression and disturbance of [Ca2+]i homeostasis might play pivotal roles in Ver-induced RPE cells apoptosis.

语种: 英语
所属项目编号: 30471865
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000289208200002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68016
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Liaoning Med Univ, Hosp 1, Dept Ophthalmol, Jinzhou 121001, Liaoning Prov, Peoples R China
2.Beijing Med Univ, Hosp 3, Dept Ophthalmol, Beijing 100191, Peoples R China

Recommended Citation:
Pang, Dong-Bo,Hong, Jing. [Ca2+]i homeostasis and caspase-3 gene expression in verapamil -induced retinal pigment epithelium cells apoptosis in vitro[J]. INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,2010,3(4):288-290.
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