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[Ca2+]i homeostasis and caspase-3 gene expression in verapamil -induced retinal pigment epithelium cells apoptosis in vitro
Pang, Dong-Bo1; Hong, Jing2
关键词[Ca2+]i homeostasis caspase-3 gene Ver-induced RPE cells apoptosis
刊名INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2010-12-18
3期:4页:288-290
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]MATRIX
英文摘要

AIM: To study caspase-3 gene expression and [Ca2+]i homeostasis in verapamil (Ver)-induced human retinal pigment epithelium (RPE) cells apoptosis.

METHODS: Ver 80mg/L was applied in cultured human RPE cells for 12, 24 and 48 hours to induce RPE cells apoptosis. The expression of apoptotic effector gene caspase-3 was assessed by reverse transcription polymerase chain reaction (RT-PCR). Single cell was measured using fluorescence indicator Fura-3/AM with MetaFluo4.5/coolsnapfx/IX70 intracellular ca(2+) fluorescence imaging system.

RESULTS: High levels of expression of caspase-3 mRNA were observed in normal RPE cells and it significantly increased after co-cultured with Ver. The fluorescence in resting RPE cells was strong and distributed throughout the cells. The nucleus appeared more fluorescent than the cytoplasm. Calcium fluorescence of RPE cells attenuated after co-cultured with Ver.

CONCLUSION: Up-regulation of caspase-3 gene expression and disturbance of [Ca2+]i homeostasis might play pivotal roles in Ver-induced RPE cells apoptosis.

语种英语
WOS记录号WOS:000289208200002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68016
专题北京大学第三临床医学院_眼科
作者单位1.Liaoning Med Univ, Hosp 1, Dept Ophthalmol, Jinzhou 121001, Liaoning Prov, Peoples R China
2.Beijing Med Univ, Hosp 3, Dept Ophthalmol, Beijing 100191, Peoples R China
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GB/T 7714
Pang, Dong-Bo,Hong, Jing. [Ca2+]i homeostasis and caspase-3 gene expression in verapamil -induced retinal pigment epithelium cells apoptosis in vitro[J]. INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,2010,3(4):288-290.
APA Pang, Dong-Bo,&Hong, Jing.(2010).[Ca2+]i homeostasis and caspase-3 gene expression in verapamil -induced retinal pigment epithelium cells apoptosis in vitro.INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,3(4),288-290.
MLA Pang, Dong-Bo,et al."[Ca2+]i homeostasis and caspase-3 gene expression in verapamil -induced retinal pigment epithelium cells apoptosis in vitro".INTERNATIONAL JOURNAL OF OPHTHALMOLOGY 3.4(2010):288-290.
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