IR@PKUHSC  > 北京大学基础医学院  > 生物物理学系
学科主题基础医学
Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat
Chen, K; Li, D; Jiang, YH; Yao, WJ; Wang, XJ; Wei, XC; Gao, J; Xie, LD; Yan, ZY; Wen, ZY; Chien, S
关键词TRAIL Tet gene expression system Jurkat apoptosis
刊名CELL RESEARCH
2004-04-01
14期:2页:161-168
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]RED-BLOOD-CELLS ; DEATH RECEPTOR ; APO-2 LIGAND ; APOPTOSIS ; GENE ; INDUCTION ; NEURAMINIDASE ; INHIBITION ; FAMILY ; DOMAIN
英文摘要

The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tet-regulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K-1 increased, and the proportion of alpha-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on the biomechanic properties of Jurkat cells.

语种英语
WOS记录号WOS:000221250900009
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68038
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Biophys, Hemorheol Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
3.NYU, Med Ctr, Dept Urol, New York, NY 10016 USA
4.Peking Univ, Dept Engn Sci & Mech, Beijing 100871, Peoples R China
5.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
6.Univ Calif San Diego, Whitaker Inst Biomed Engn, La Jolla, CA 92093 USA
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GB/T 7714
Chen, K,Li, D,Jiang, YH,et al. Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat[J]. CELL RESEARCH,2004,14(2):161-168.
APA Chen, K.,Li, D.,Jiang, YH.,Yao, WJ.,Wang, XJ.,...&Chien, S.(2004).Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat.CELL RESEARCH,14(2),161-168.
MLA Chen, K,et al."Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat".CELL RESEARCH 14.2(2004):161-168.
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