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学科主题: 基础医学
题名:
Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat
作者: Chen, K; Li, D; Jiang, YH; Yao, WJ; Wang, XJ; Wei, XC; Gao, J; Xie, LD; Yan, ZY; Wen, ZY; Chien, S
关键词: TRAIL ; Tet gene expression system ; Jurkat ; apoptosis
刊名: CELL RESEARCH
发表日期: 2004-04-01
卷: 14, 期:2, 页:161-168
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: RED-BLOOD-CELLS ; DEATH RECEPTOR ; APO-2 LIGAND ; APOPTOSIS ; GENE ; INDUCTION ; NEURAMINIDASE ; INHIBITION ; FAMILY ; DOMAIN
英文摘要:

The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tet-regulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K-1 increased, and the proportion of alpha-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on the biomechanic properties of Jurkat cells.

语种: 英语
WOS记录号: WOS:000221250900009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68038
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Biophys, Hemorheol Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
3.NYU, Med Ctr, Dept Urol, New York, NY 10016 USA
4.Peking Univ, Dept Engn Sci & Mech, Beijing 100871, Peoples R China
5.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
6.Univ Calif San Diego, Whitaker Inst Biomed Engn, La Jolla, CA 92093 USA

Recommended Citation:
Chen, K,Li, D,Jiang, YH,et al. Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat[J]. CELL RESEARCH,2004,14(2):161-168.
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