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学科主题: 临床医学
题名:
Effects of integrin-linked kinase on human corpus cavernosum smooth muscle cell cytoskeletal organisation
作者: Hao, Y. -C.1; Yu, L. -P.1; Li, Q.1; Zhang, X. -W.1; Zhao, Y. -P.2; He, P. -Y.3; Xu, T.1; Wang, X. -F.1
关键词: Erectile dysfunction ; gene therapy ; integrin-linked kinase ; siRNA
刊名: ANDROLOGIA
发表日期: 2013-04-01
DOI: 10.1111/j.1439-0272.2012.01313.x
卷: 45, 期:2, 页:78-85
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology
研究领域[WOS]: Endocrinology & Metabolism
关键词[WOS]: EXPRESSION INCREASES ; ERECTILE DYSFUNCTION ; PENILE ERECTION ; CONTRACTION ; ILK ; PROGRESSION ; MIGRATION ; ADHESION ; PROTEIN ; TARGET
英文摘要:

We investigated the effects of integrin-linked kinase (ILK) on the in vitro attachment, spreading, migration and microfilament dynamics of human corpus cavernosum smooth muscle cells. ILK small interfering RNA (siRNA) was used to transfect human corpus cavernosum smooth muscle cells; and cell attachment, spreading and migration were assessed. Additionally, microfilament dynamics were evaluated using Alexa Fluor 488 and phalloidin staining. We found that ILK gene knock-down significantly inhibited human corpus cavernosum smooth muscle cell attachment, spreading and migration. Moreover, blocking the expression of ILK disturbed actin cytoskeleton reorganisation and morphology in human corpus cavernosum smooth muscle cells. These results show that the targeting of ILK with siRNA significantly inhibited cell attachment, spreading, migration and microfilament dynamics in human corpus cavernosum smooth muscle cells. These findings indicate that ILK might be a potential therapeutic molecular target for the treatment of erectile dysfunction.

语种: 英语
所属项目编号: RDB2010-16
项目资助者: Peking University People&prime ; s Hospital Research and a Development Funds
WOS记录号: WOS:000315398900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68086
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Peking Univ Peoples Hosp, Reprod Med Ctr, Beijing 100044, Peoples R China
3.Peking Univ Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China

Recommended Citation:
Hao, Y. -C.,Yu, L. -P.,Li, Q.,et al. Effects of integrin-linked kinase on human corpus cavernosum smooth muscle cell cytoskeletal organisation[J]. ANDROLOGIA,2013,45(2):78-85.
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