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学科主题临床医学
Effects of integrin-linked kinase on human corpus cavernosum smooth muscle cell cytoskeletal organisation
Hao, Y. -C.1; Yu, L. -P.1; Li, Q.1; Zhang, X. -W.1; Zhao, Y. -P.2; He, P. -Y.3; Xu, T.1; Wang, X. -F.1
关键词Erectile dysfunction gene therapy integrin-linked kinase siRNA
刊名ANDROLOGIA
2013-04-01
DOI10.1111/j.1439-0272.2012.01313.x
45期:2页:78-85
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology
资助者Peking University People&prime ; s Hospital Research and a Development Funds ; Peking University People&prime ; s Hospital Research and a Development Funds
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]EXPRESSION INCREASES ; ERECTILE DYSFUNCTION ; PENILE ERECTION ; CONTRACTION ; ILK ; PROGRESSION ; MIGRATION ; ADHESION ; PROTEIN ; TARGET
英文摘要

We investigated the effects of integrin-linked kinase (ILK) on the in vitro attachment, spreading, migration and microfilament dynamics of human corpus cavernosum smooth muscle cells. ILK small interfering RNA (siRNA) was used to transfect human corpus cavernosum smooth muscle cells; and cell attachment, spreading and migration were assessed. Additionally, microfilament dynamics were evaluated using Alexa Fluor 488 and phalloidin staining. We found that ILK gene knock-down significantly inhibited human corpus cavernosum smooth muscle cell attachment, spreading and migration. Moreover, blocking the expression of ILK disturbed actin cytoskeleton reorganisation and morphology in human corpus cavernosum smooth muscle cells. These results show that the targeting of ILK with siRNA significantly inhibited cell attachment, spreading, migration and microfilament dynamics in human corpus cavernosum smooth muscle cells. These findings indicate that ILK might be a potential therapeutic molecular target for the treatment of erectile dysfunction.

语种英语
所属项目编号RDB2010-16
资助者Peking University People&prime ; s Hospital Research and a Development Funds ; Peking University People&prime ; s Hospital Research and a Development Funds
WOS记录号WOS:000315398900002
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68086
专题北京大学第二临床医学院_泌尿外科
作者单位1.Peking Univ Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Peking Univ Peoples Hosp, Reprod Med Ctr, Beijing 100044, Peoples R China
3.Peking Univ Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China
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Hao, Y. -C.,Yu, L. -P.,Li, Q.,et al. Effects of integrin-linked kinase on human corpus cavernosum smooth muscle cell cytoskeletal organisation[J]. ANDROLOGIA,2013,45(2):78-85.
APA Hao, Y. -C..,Yu, L. -P..,Li, Q..,Zhang, X. -W..,Zhao, Y. -P..,...&Wang, X. -F..(2013).Effects of integrin-linked kinase on human corpus cavernosum smooth muscle cell cytoskeletal organisation.ANDROLOGIA,45(2),78-85.
MLA Hao, Y. -C.,et al."Effects of integrin-linked kinase on human corpus cavernosum smooth muscle cell cytoskeletal organisation".ANDROLOGIA 45.2(2013):78-85.
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