北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Metformin Suppresses Prostaglandin E2-Induced Cytochrome P450 Aromatase Gene Expression and Activity via Stimulation of AMP-Activated Protein Kinase in Human Endometriotic Stromal Cells
作者: Zhou, Yan1; Xu, Jia-Ning1; Zeng, Cheng1; Li, Xin1; Zhou, Ying-Fang1; Qi, Yu2; Xue, Qing1
关键词: metformin ; CYP19A1 ; AMPK ; CREB ; endometriosis
刊名: REPRODUCTIVE SCIENCES
发表日期: 2015-09-01
DOI: 10.1177/1933719115590664
卷: 22, 期:9, 页:1162-1170
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: STEROIDOGENIC FACTOR-I ; POLYCYSTIC-OVARY-SYNDROME ; ACUTE REGULATORY PROTEIN ; GROWTH-FACTOR-I ; GRANULOSA-CELLS ; LEYDIG-CELLS ; CYP19 GENE ; DISEASE ; BREAST ; TRANSCRIPTION
英文摘要:

Background: Cytochrome P450 aromatase (encoded by the CYP19A1/aromatase gene) plays a critical physiologic role in endometriosis. Metformin is known to suppress prostaglandin E2 (PGE2)-induced CYP19A1 messenger RNA (mRNA) expression in human endometriotic stromal cells (ESCs). However, the possible mechanism behind this suppression remains to be determined.

Methods: In this study, ESCs were cultured with metformin, PGE2, and adenosine monophosphate (AMP)-activated protein kinase (AMPK) inhibitors. Expression of CYP19A1 mRNA and aromatase activity were measured by quantitative polymerase chain reaction and aromatase activity assay, respectively. The binding of the cyclic AMP response element-binding (CREB) protein to CYP19A1 promoter II (PII) was assessed by chromatin immunoprecipitation assay.

Results: We demonstrated that metformin downregulated the expression of aromatase mRNA (32%) and activity (25%) stimulated by PGE2 (4.18-fold and 2.14-fold) in ESCs via stimulation of AMPK. Following PGE2 treatment, there was a marked increase in CREB binding to aromatase PII, while metformin attenuated the above-mentioned stimulation by 67%.

Conclusion: Metformin could inhibit PGE2-induced CYP19A1 mRNA expression and aromatase activity via AMPK activation and inhibition of CREB to CYP19A1 PII in human ESCs. The results of the present study suggest that metformin may have unique therapeutic potential as an antiendometriotic drug in the future.

语种: 英语
所属项目编号: 81270674 ; 7132204
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Beijing, China
WOS记录号: WOS:000359419900014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68095
Appears in Collections:北京大学第一临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Cent Lab, Beijing 100034, Peoples R China

Recommended Citation:
Zhou, Yan,Xu, Jia-Ning,Zeng, Cheng,et al. Metformin Suppresses Prostaglandin E2-Induced Cytochrome P450 Aromatase Gene Expression and Activity via Stimulation of AMP-Activated Protein Kinase in Human Endometriotic Stromal Cells[J]. REPRODUCTIVE SCIENCES,2015,22(9):1162-1170.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, Yan]'s Articles
[Xu, Jia-Ning]'s Articles
[Zeng, Cheng]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, Yan]‘s Articles
[Xu, Jia-Ning]‘s Articles
[Zeng, Cheng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace