北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 药剂科  > 期刊论文
学科主题: 临床医学
题名:
Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma
作者: Wang, Jiang; Jiang, Yao; Wang, Yingwu; Zhao, Xia; Cui, Yimin; Gu, Jingkai
关键词: aildenafil ; LC-MS/MS ; pharmacokinetics
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2007-05-09
DOI: 10.1016/j.jpba.2007.01.017
卷: 44, 期:1, 页:231-235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: SYNTHETIC PHOSPHODIESTERASE-5 INHIBITORS ; DIETARY-SUPPLEMENTS ; SILDENAFIL VIAGRA(R) ; V INHIBITOR ; VARDENAFIL ; MS ; EXTRACTION ; METABOLITE ; DA-8159 ; URINE
英文摘要:

A simple, sensitive and specific liquid chromatography/tandem mass spectrometry method for the quantitation of aildenafil, a new phosphodiesterase V inhibitor, in human plasma is presented. The analyte and internal standard, sildenafil, were extracted by a one-step liquid-liquid extraction in alkaline conditions and separated on a C-18 column using ammonia: 10mM ammonium acetate buffer:methanol (0.1: 15:85, v/v/v) as the mobile phase. The detection by an API 4000 triple quadrupole mass spectrometer in multiple-reaction monitoring mode was completed within 2.5 min. The calibration curve exhibited a linear dynamic-range of 0.05-100 ng/ml with a 10 pg/ml limit of detection. The intra- and inter-day precisions measured as relative standard deviation were within 8.04% and 5.72%, respectively. This method has been used in a pharmacokinetic study of aildenafil in healthy male volunteers each given an oral administration of one of the three dosages. (c) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000246350100031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68099
Appears in Collections:北京大学第一临床医学院_药剂科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Jilin Univ, Coll Life Sci, Res Ctr Drug & Metab, Changchun 130023, Peoples R China
2.Peking Univ, Hosp 1, Dept Pharm, Beijing 100034, Peoples R China

Recommended Citation:
Wang, Jiang,Jiang, Yao,Wang, Yingwu,et al. Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,44(1):231-235.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Jiang]'s Articles
[Jiang, Yao]'s Articles
[Wang, Yingwu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Jiang]‘s Articles
[Jiang, Yao]‘s Articles
[Wang, Yingwu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace