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学科主题: 临床医学
题名:
Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma
作者: Wang, Jiang; Jiang, Yao; Wang, Yingwu; Zhao, Xia; Cui, Yimin; Gu, Jingkai
关键词: aildenafil ; LC-MS/MS ; pharmacokinetics
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2007-05-09
DOI: 10.1016/j.jpba.2007.01.017
卷: 44, 期:1, 页:231-235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: SYNTHETIC PHOSPHODIESTERASE-5 INHIBITORS ; DIETARY-SUPPLEMENTS ; SILDENAFIL VIAGRA(R) ; V INHIBITOR ; VARDENAFIL ; MS ; EXTRACTION ; METABOLITE ; DA-8159 ; URINE
英文摘要:

A simple, sensitive and specific liquid chromatography/tandem mass spectrometry method for the quantitation of aildenafil, a new phosphodiesterase V inhibitor, in human plasma is presented. The analyte and internal standard, sildenafil, were extracted by a one-step liquid-liquid extraction in alkaline conditions and separated on a C-18 column using ammonia: 10mM ammonium acetate buffer:methanol (0.1: 15:85, v/v/v) as the mobile phase. The detection by an API 4000 triple quadrupole mass spectrometer in multiple-reaction monitoring mode was completed within 2.5 min. The calibration curve exhibited a linear dynamic-range of 0.05-100 ng/ml with a 10 pg/ml limit of detection. The intra- and inter-day precisions measured as relative standard deviation were within 8.04% and 5.72%, respectively. This method has been used in a pharmacokinetic study of aildenafil in healthy male volunteers each given an oral administration of one of the three dosages. (c) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000246350100031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68099
Appears in Collections:北京大学第一临床医学院_药剂科_期刊论文

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作者单位: 1.Jilin Univ, Coll Life Sci, Res Ctr Drug & Metab, Changchun 130023, Peoples R China
2.Peking Univ, Hosp 1, Dept Pharm, Beijing 100034, Peoples R China

Recommended Citation:
Wang, Jiang,Jiang, Yao,Wang, Yingwu,et al. Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,44(1):231-235.
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