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学科主题临床医学
Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma
Wang, Jiang; Jiang, Yao; Wang, Yingwu; Zhao, Xia; Cui, Yimin; Gu, Jingkai
关键词aildenafil LC-MS/MS pharmacokinetics
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2007-05-09
DOI10.1016/j.jpba.2007.01.017
44期:1页:231-235
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]SYNTHETIC PHOSPHODIESTERASE-5 INHIBITORS ; DIETARY-SUPPLEMENTS ; SILDENAFIL VIAGRA(R) ; V INHIBITOR ; VARDENAFIL ; MS ; EXTRACTION ; METABOLITE ; DA-8159 ; URINE
英文摘要

A simple, sensitive and specific liquid chromatography/tandem mass spectrometry method for the quantitation of aildenafil, a new phosphodiesterase V inhibitor, in human plasma is presented. The analyte and internal standard, sildenafil, were extracted by a one-step liquid-liquid extraction in alkaline conditions and separated on a C-18 column using ammonia: 10mM ammonium acetate buffer:methanol (0.1: 15:85, v/v/v) as the mobile phase. The detection by an API 4000 triple quadrupole mass spectrometer in multiple-reaction monitoring mode was completed within 2.5 min. The calibration curve exhibited a linear dynamic-range of 0.05-100 ng/ml with a 10 pg/ml limit of detection. The intra- and inter-day precisions measured as relative standard deviation were within 8.04% and 5.72%, respectively. This method has been used in a pharmacokinetic study of aildenafil in healthy male volunteers each given an oral administration of one of the three dosages. (c) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000246350100031
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68099
Collection北京大学第一临床医学院_药剂科
作者单位1.Jilin Univ, Coll Life Sci, Res Ctr Drug & Metab, Changchun 130023, Peoples R China
2.Peking Univ, Hosp 1, Dept Pharm, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jiang,Jiang, Yao,Wang, Yingwu,et al. Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,44(1):231-235.
APA Wang, Jiang,Jiang, Yao,Wang, Yingwu,Zhao, Xia,Cui, Yimin,&Gu, Jingkai.(2007).Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,44(1),231-235.
MLA Wang, Jiang,et al."Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 44.1(2007):231-235.
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