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学科主题: 基础医学
题名:
MiR-499 Regulates Cell Proliferation and Apoptosis during Late-Stage Cardiac Differentiation via Sox6 and Cyclin D1
作者: Li, Xianhui1; Wang, Jiaji1; Jia, Zhuqing1; Cui, Qinghua2; Zhang, Chenguang1; Wang, Weiping1; Chen, Ping1; Ma, Kangtao1; Zhou, Chunyan1
刊名: PLOS ONE
发表日期: 2013-09-11
DOI: 10.1371/journal.pone.0074504
卷: 8, 期:9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: DOWN-REGULATION ; EXPRESSION ; HEART ; GENE ; REGENERATION ; MICRORNA ; DEATH
英文摘要:

Background: MiR-499 is a cardiac-abundant miRNA. However, the biological functions of miR-499 in differentiated cardiomyocytes or in the cardiomyocyte differentiation process is not very clear. Sox6 is believed to be one of its targets, and is also believed to play a role in cardiac differentiation. Therefore, our aim was to investigate the association between Sox6 and miR-499 during cardiac differentiation.

Methodology/Principal Findings: Using a well-established in vitro cardiomyocyte differentiation system, mouse P19CL6 cells, we found that miR-499 was highly expressed in the late stage of cardiac differentiation. In cells stably transfected with miR-499 (P-499 cells), it was found that miR-499 could promote the differentiation into cardiomyocytes at the early stage of cardiac differentiation. Notably, cell viability assay, EdU incorporation assay, and cell cycle profile analysis all showed that the P-499 cells displayed the distinctive feature of hyperplastic growth. Further investigation confirmed that miR-499 could promote neonatal rat cardiomyocyte proliferation. MiR-499 knockdown enhanced apoptosis in the late differentiation stage in P19CL6 cells, but overexpression of miR-499 resulted in a decrease in the apoptosis rate. Sox6 was identified as a direct target of miR-499 and its expression was detected from day 8 or day 10 of cardiac differentiation of P19CL6 cells. Sox6 played a role in cell viability, inhibited cell proliferation and promoted cell apoptosis in P19CL6 cells and cardiomyocytes. The overexpression of Sox6 could reverse the proliferation and anti-apoptosis effects of miR-499. It was also found that miR-499 might exert its function by regulating cyclin D1 via its influence on Sox6.

Conclusions/Significance: miR-499 probably regulates the proliferation and apoptosis of P19CL6 cells in the late stage of cardiac differentiation via its effects on Sox6 and cyclin D1.

语种: 英语
所属项目编号: 81070112 ; 81071675 ; 81170713 ; 20110001130001 ; 5122021 ; B07001
项目资助者: National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Natural Science Foundation of Beijing, China ; Leading Academic Discipline Project of Beijing Education Bureau ; 111 Project of China
WOS记录号: WOS:000326734500098
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68107
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Key Lab Mol Cardiovasc Sci,Minist Educ China, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biomed Informat, Beijing 100871, Peoples R China

Recommended Citation:
Li, Xianhui,Wang, Jiaji,Jia, Zhuqing,et al. MiR-499 Regulates Cell Proliferation and Apoptosis during Late-Stage Cardiac Differentiation via Sox6 and Cyclin D1[J]. PLOS ONE,2013,8(9).
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