学科主题基础医学
Mature hepatocytes obtained in ES cells directional differentiation system in vitro
Hu, AB; Cai, JY; Zheng, QC; Hong, A; He, XQ; Dai, Y; Shan, Y; Pan, YL; Li, LS
关键词ES cells hepatocytes differentiation
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2004-06-01
31期:6页:518-522
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]EMBRYONIC STEM-CELLS ; LIVER DEVELOPMENT ; EXPRESSION ; INITIATION ; INVITRO
英文摘要

The mature differentiation of ES cells into hepatocytes and its differentiation ratio were studied in vitro. First, the BALB/c mouse ES cells were cultured for 5 days to develop into EBs in EBs culture medium. Then, the cell growth factors such as transform growth factor (TGF), basic fibroblast growth factor ( bFGF) and hepatocyte growth factor (HGF) were added into the medium to induce the differentiation of EBs cells into hepatocytes. During the culture medium, the reverse transcriptase-polymerase chain reaction (RT-PCR), immunocytochemistry (ICC) and radioinummoassay (RIA) were used to determine the expression of hepatic genes and proteins, such as (x-fetoprotein (AFP albumin (ALB), glucose-6-phosphatase (G6P), tyrosineaminotransferase ( TAT), cytokeratin 8 (CK8) cytokeratin 18 (CK18) and urea, to analyse the hepatic differentiation. At last, the hepatic differentiation ratio was also determined by counting the ICC-positive cells. In results, the mRNA of AFP, ALB, G6P and TAT didn′t respectively expressed till at day3, day9, day11 and day13. AFP, ALB and urea in culture medium were not detected till at day8, day11 and day12 with a concentration of (3.4 +/- 0.6) mug/L, (0.21 +/- 0.04) mg/L and (8.3 +/- 1.4) mumol/L. Hepatic proteins such as AFP, ALB, CK8, CK18 didn′t respectively expressed in cytoplasm till at day7, day9, day9, day11 and ICC-positive cells had morphological structures consistent with mouse primary hepatocytes. Hepatic differentiation ratio was determined at day19 with ratio of 32%. In conclusions, hepatocytes could be obtained in ES cells differentiation system and get matured with differentiation ratio of 32% when growth factors such as TGF, bFGF and HGF added into culture medium. This may produce a new resource of hepatocytes in liver engineering and hepatocytes transplantation for treating hepatic failure.

语种中文
WOS记录号WOS:000223058500007
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68110
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Sun Yat Sen Univ, Coll Med, Guangzhou 510080, Peoples R China
2.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
3.Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Peoples R China
4.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Gen Surg, Wuhan 430022, Peoples R China
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Hu, AB,Cai, JY,Zheng, QC,et al. Mature hepatocytes obtained in ES cells directional differentiation system in vitro[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(6):518-522.
APA Hu, AB.,Cai, JY.,Zheng, QC.,Hong, A.,He, XQ.,...&Li, LS.(2004).Mature hepatocytes obtained in ES cells directional differentiation system in vitro.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(6),518-522.
MLA Hu, AB,et al."Mature hepatocytes obtained in ES cells directional differentiation system in vitro".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.6(2004):518-522.
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