北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Metabolites identification of glycycoumarin, a major bioactive coumarin from licorice in rats
作者: Wang, Qi; Qiao, Xue; Liu, Chun-fang; Ji, Shuai; Feng, Lin-min; Qian, Yi; Guo, De-an; Ye, Min
关键词: Glycycoumarin ; Glycyrrhiza uralensis ; LC/MS ; Metabolites identification ; NMR
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2014-09-01
DOI: 10.1016/j.jpba.2014.06.001
卷: 98, 页:287-295
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: HUMAN LIVER-MICROSOMES ; HOPS HUMULUS-LUPULUS ; GLYCYRRHIZA-URALENSIS ; MASS-SPECTROMETRY ; PRENYLATED FLAVONOIDS ; HERBAL MEDICINE ; 8-PRENYLNARINGENIN ; ISOXANTHOHUMOL ; CONSTITUENTS ; INHIBITION
英文摘要:

Glycycoumarin is a major bioactive coumarin of licorice (Glycyrrhiza uralensis), one of the most popular herbal medicines worldwide. In this work, the metabolism of glycycoumarin in rats was investigated. After oral administration of 40 mg/kg glycycoumarin, 4 and 10 metabolites were respectively detected in rats plasma and urine samples by liquid chromatography coupled with mass spectrometry (LC/MS). These metabolites were tentatively characterized by analyzing their tandem mass spectra and high-resolution mass spectra, and the structures of glucuronides were confirmed by p-glucuronidase hydrolysis. Glycycoumarin mainly undertakes hydroxylation and glucuronidation metabolism, accompanied by hydrogenation and dehydrogenation as minor reactions. Two hydroxylated metabolites, 4 ′′-hydroxyl glycycoumarin and 5 ′′-hydroxyl glycycoumarin, were obtained by microbial transformation of Syncephalastrum racemosum AS 3.264, and their structures were fully identified by 1D and 2D NMR. Both metabolites are new compounds. Furthermore, they were proved to be catalyzed by P450 enzymes by rat liver microsomes incubation experiments. Finally, a metabolic pathway of glycycoumarin in rats was proposed. This is the first systematic study on metabolites identification of glycycoumarin. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81173644 ; 81222054 ; NCET-11-0019
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education
WOS记录号: WOS:000339859800038
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68112
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Qi,Qiao, Xue,Liu, Chun-fang,et al. Metabolites identification of glycycoumarin, a major bioactive coumarin from licorice in rats[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,98:287-295.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Qi]'s Articles
[Qiao, Xue]'s Articles
[Liu, Chun-fang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Qi]‘s Articles
[Qiao, Xue]‘s Articles
[Liu, Chun-fang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace