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Metabolites identification of glycycoumarin, a major bioactive coumarin from licorice in rats
Wang, Qi; Qiao, Xue; Liu, Chun-fang; Ji, Shuai; Feng, Lin-min; Qian, Yi; Guo, De-an; Ye, Min
关键词Glycycoumarin Glycyrrhiza uralensis LC/MS Metabolites identification NMR
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2014-09-01
DOI10.1016/j.jpba.2014.06.001
98页:287-295
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]HUMAN LIVER-MICROSOMES ; HOPS HUMULUS-LUPULUS ; GLYCYRRHIZA-URALENSIS ; MASS-SPECTROMETRY ; PRENYLATED FLAVONOIDS ; HERBAL MEDICINE ; 8-PRENYLNARINGENIN ; ISOXANTHOHUMOL ; CONSTITUENTS ; INHIBITION
英文摘要

Glycycoumarin is a major bioactive coumarin of licorice (Glycyrrhiza uralensis), one of the most popular herbal medicines worldwide. In this work, the metabolism of glycycoumarin in rats was investigated. After oral administration of 40 mg/kg glycycoumarin, 4 and 10 metabolites were respectively detected in rats plasma and urine samples by liquid chromatography coupled with mass spectrometry (LC/MS). These metabolites were tentatively characterized by analyzing their tandem mass spectra and high-resolution mass spectra, and the structures of glucuronides were confirmed by p-glucuronidase hydrolysis. Glycycoumarin mainly undertakes hydroxylation and glucuronidation metabolism, accompanied by hydrogenation and dehydrogenation as minor reactions. Two hydroxylated metabolites, 4 ′′-hydroxyl glycycoumarin and 5 ′′-hydroxyl glycycoumarin, were obtained by microbial transformation of Syncephalastrum racemosum AS 3.264, and their structures were fully identified by 1D and 2D NMR. Both metabolites are new compounds. Furthermore, they were proved to be catalyzed by P450 enzymes by rat liver microsomes incubation experiments. Finally, a metabolic pathway of glycycoumarin in rats was proposed. This is the first systematic study on metabolites identification of glycycoumarin. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000339859800038
项目编号81173644 ; 81222054 ; NCET-11-0019
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68112
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
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Wang, Qi,Qiao, Xue,Liu, Chun-fang,et al. Metabolites identification of glycycoumarin, a major bioactive coumarin from licorice in rats[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,98:287-295.
APA Wang, Qi.,Qiao, Xue.,Liu, Chun-fang.,Ji, Shuai.,Feng, Lin-min.,...&Ye, Min.(2014).Metabolites identification of glycycoumarin, a major bioactive coumarin from licorice in rats.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,98,287-295.
MLA Wang, Qi,et al."Metabolites identification of glycycoumarin, a major bioactive coumarin from licorice in rats".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 98(2014):287-295.
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