IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Effect of berberine on hepatocyte proliferation, inducible nitric oxide synthase expression, cytochrome P450 2E1 and 1A2 activities in diethylnitrosamine- and phenobarbital-treated rats
Zhao, Xuan; Zhang, Jun-Jie; Wang, Xin; Bu, Xiu-Yun; Lou, Ya-Qing; Zhang, Guo-Liang
关键词Berbetine Diethylnitrosamine (DEN) Phenobarbital Cytochrome P450 2E1 (CYP2E1) Cytochrome P450 1A2 (CYP1A2)
刊名BIOMEDICINE & PHARMACOTHERAPY
2008-11-01
DOI10.1016/j.biopha.2007.02.009
62期:9页:567-572
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]LIVER-INJURY ; N-NITROSODIETHYLAMINE ; CARCINOGENESIS ; MICE ; CONSTITUENTS ; INHIBITION ; METABOLISM ; GOLDENSEAL ; MICROSOMES ; OXIDATION
英文摘要

This study investigated the effect of berberine on the early phase of hepatocarcinogenesis stimulated by diethylnitrosamine (DEN, 150 mg/kg, 4 weeks) plus phenobarbital (PB, 75 mg/kg 7 days) in rats. The expressions of proliferating cell nuclear antigen (PCNA) and inducible nitric oxide synthase (iNOS) were evaluated by immunohistochemistry. The activities of CYP isoenzymes were analyzed using different probe drugs including chlorzoxazone (CYP2E1) and phenacetin (CYP1A2) by high-performance liquid chromatography (HPLC) in vivo or in vitro. Results showed that the expressions of PCNA and iNOS were induced by DEN plus PB in liver tissues. Oral administration of berberine (50 mg/kg) inhibited the hepatocyte proliferation and iNOS expression, decreased cytochrome P450 content, inhibited activities of CYP2E1 and CYP1A2 in DEN-plus-PB-treated rats in vivo. Moreover. berberine (10, 50 and 100 mu M) inhibited the activities of CYP2E1 and CYP1A2 in microsomes isolated from DEN-plus-PB-treated rats in vitro, suggesting that anti-hepatocarcinogenetic potential of berberine might be due to inhibiting oxidative metabolic activities of CYP2E1 and CYP1A2. and decreasing NO production in rats. (C) 2007 Elsevier Masson SAS. All rights reserved.

语种英语
WOS记录号WOS:000261271900003
项目编号30171097 ; 30371665 ; Z0004105040311
资助机构National Natural Science Foundation of China ; Project of Beijing Biotechnology Foundation
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68122
专题北京大学基础医学院_药理学系
作者单位Beijing Univ, Basic Med Sch, Dept Pharmacol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Xuan,Zhang, Jun-Jie,Wang, Xin,et al. Effect of berberine on hepatocyte proliferation, inducible nitric oxide synthase expression, cytochrome P450 2E1 and 1A2 activities in diethylnitrosamine- and phenobarbital-treated rats[J]. BIOMEDICINE & PHARMACOTHERAPY,2008,62(9):567-572.
APA Zhao, Xuan,Zhang, Jun-Jie,Wang, Xin,Bu, Xiu-Yun,Lou, Ya-Qing,&Zhang, Guo-Liang.(2008).Effect of berberine on hepatocyte proliferation, inducible nitric oxide synthase expression, cytochrome P450 2E1 and 1A2 activities in diethylnitrosamine- and phenobarbital-treated rats.BIOMEDICINE & PHARMACOTHERAPY,62(9),567-572.
MLA Zhao, Xuan,et al."Effect of berberine on hepatocyte proliferation, inducible nitric oxide synthase expression, cytochrome P450 2E1 and 1A2 activities in diethylnitrosamine- and phenobarbital-treated rats".BIOMEDICINE & PHARMACOTHERAPY 62.9(2008):567-572.
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