|ER-alpha 36, a novel variant of estrogen receptor alpha, is involved in EGFR-related carcinogenesis in endometrial cancer|
|Tu, Bin-Bin1; Lin, Sheng-Li2; Yan, Li-Ying1; Wang, Zhao-Yi3; Sun, Qing-Yuan2; Qiao, Jie1|
|关键词||endometrial cancer epidermal growth factor receptor estrogen receptor-alpha 36 estrogen receptor-alpha 66 MAPK/ERK pathway|
|刊名||AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Obstetrics & Gynecology|
|研究领域[WOS]||Obstetrics & Gynecology|
|关键词[WOS]||GROWTH-FACTOR RECEPTOR ; ACTIVATED PROTEIN-KINASE ; ER-ALPHA ; BREAST-CANCER ; EXPRESSION ; CELLS ; PHOSPHORYLATION ; CARCINOMA ; PATHWAY|
OBJECTIVE: To explore the role of estrogen receptor-alpha 36 (ER-alpha 36) in epidermal growth factor receptor (EGFR)-related carcinogenesis in endometrial cancer.
STUDY DESIGN: The expression of ER-alpha 36, EGFR, and phospho-extracellular signal-regulated kinase was analyzed using immunohistochemistry in endometrial cancer samples. The cellular localization of ER-alpha 36 and EGFR was determined using immunofluorescence in the endometrial cancer Hec1A cells. The level of phospho-extracellular signal- regulated kinase of Hec1A cells was determined using Western blotting after treatment with epidermal growth factor.
RESULTS: Positive rate of ER-alpha 36 was increased in high-stage (P = .03) and high-grade (P = .224) endometrial cancer; expression of ER-alpha 36 and EGFR exhibited a significant positive correlation (r = 0.334, P = .025) and they showed substantial colocalization on the plasma membrane of glandular cells; phospho-extracellular signal-regulated kinase positive rate in ER-alpha 36 positive group and EGFR positive group was higher than that of ER-alpha 36 negative group (P = .014) and EGFR negative group (P = .016); finally, ER-alpha 36 mediated epidermal growth factor-stimulated extracellular signal-regulated kinase activation in Hec1A cells.
CONCLUSION: ER-alpha 36 mediates EGFR-related extracellular signal-regulated kinase activation in endometrial cancer.
|作者单位||1.Peking Univ, Dept Obstet & Gynecol, Reprod Med Ctr, Hosp 3, Beijing 100191, Peoples R China|
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
3.Creighton Univ, Sch Med, Dept Med Microbiol & Immunol, Omaha, NE USA
|Tu, Bin-Bin,Lin, Sheng-Li,Yan, Li-Ying,et al. ER-alpha 36, a novel variant of estrogen receptor alpha, is involved in EGFR-related carcinogenesis in endometrial cancer[J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY,2011,205(3).|
|APA||Tu, Bin-Bin,Lin, Sheng-Li,Yan, Li-Ying,Wang, Zhao-Yi,Sun, Qing-Yuan,&Qiao, Jie.(2011).ER-alpha 36, a novel variant of estrogen receptor alpha, is involved in EGFR-related carcinogenesis in endometrial cancer.AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY,205(3).|
|MLA||Tu, Bin-Bin,et al."ER-alpha 36, a novel variant of estrogen receptor alpha, is involved in EGFR-related carcinogenesis in endometrial cancer".AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY 205.3(2011).|