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学科主题: 临床医学
题名:
BST2/Tetherin Inhibits Dengue Virus Release from Human Hepatoma Cells
作者: Pan, Xiao-Ben1; Han, Jin-Chao1; Cong, Xu1; Wei, Lai1,2
刊名: PLOS ONE
发表日期: 2012-12-07
DOI: 10.1371/journal.pone.0051033
卷: 7, 期:12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: HUMAN-IMMUNODEFICIENCY-VIRUS ; WEST NILE VIRUS ; HEPATITIS-C VIRUS ; IN-VIVO ; PARTICLE RELEASE ; INTERFERON-ALPHA ; HIV-1 VPU ; INFECTION ; TETHERIN ; PROTEIN
英文摘要:

Type I interferons (IFN) have been shown to play an important role for inhibiting Dengue virus (DENV) infection. Identifying IFN-induced cellular proteins are essential for understanding its mechanisms against DENV. Here we established stable Huh7-derived cell lines expressing the IFN-induced cell membrane protein BST2 (Huh7-BST2) or its variant bearing a V5 tag at the C-terminal (Huh7-BST5CV5). These cell lines were infected with DENV to determine proteins modulating their anti-DENV response. We found that expression of BST2 did not affect the efficiency of DENV infection and intracellular replication. Rather, it significantly reduced the virion yield of the infected cells, particularly at low MOI infection. In addition, BST2 also decreased the foci formation and the size of infectious foci in cultured Huh7 monolayers with media containing methocellulose. The addition of the V5 tag at C-terminal inhibited the GPI modification of BST2 and blocked its shift from endoplasm to cytoplastic membrane. BST2CV5 did not affect DENV infection and foci formation in Huh7 cells but reduced virion yield by 1 log at low MOI infection. Interestingly, intracellular BST2CV5 expression was reduced by high level of DENV production.

Conclusion: Our results imply that BST2 is a functional mediator of the IFN response against DENV infection. BST2 inhibits the release of DENV virions from Huh7 cells and limits viral cell-to-cell transmission. BST2CV5 variant is unable to inhibit DENV release but impairs viral infection in cells.

语种: 英语
所属项目编号: 30972605 ; 30700697 ; BMU20110272
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University (NCET)
WOS记录号: WOS:000312064100056
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68127
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis C & Immunotherapy Liver, Beijing 100871, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Infect Dis, Beijing 100871, Peoples R China

Recommended Citation:
Pan, Xiao-Ben,Han, Jin-Chao,Cong, Xu,et al. BST2/Tetherin Inhibits Dengue Virus Release from Human Hepatoma Cells[J]. PLOS ONE,2012,7(12).
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