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学科主题: 基础医学
题名:
The presence of active Cdk5 associated with p35 in astrocytes and its important role in process elongation of scratched astrocyte
作者: He, Yi; Li, Hui-Li; Xie, Wei-Yan; Yang, Chun-Zhang; Yu, Albert Cheung Hoi; Wang, Yun
关键词: Cdk5/p35 ; astrocyte ; injury ; hypertrophy ; scratch
刊名: GLIA
发表日期: 2007-04-15
DOI: 10.1002/glia.20485
卷: 55, 期:6, 页:573-583
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: CYCLIN-DEPENDENT KINASE-5 ; REACTIVE ASTROCYTES ; NERVOUS-SYSTEM ; GROWTH-FACTOR ; REGULATORY SUBUNIT ; CELL ; EXPRESSION ; INJURY ; DIFFERENTIATION ; MIGRATION
英文摘要:

Cyclin-dependent kinase 5 (Cdk5) is a unique member of the Cdk family its kinase activity requires association with its activator, p35 or p39. p35 is the strongest and best characterized activator. Previous studies showed that p35 is a neuron-specific protein that restricts Cdk5 activity in neurons. However, a high expression level of Cdk5 is found in astrocytes, which raises the possibility that astrocytic Cdk5 is functional. Here we show the presence of functional Cdk5 associated with p35 in astrocytes and demonstrate its important role in process elongation of scratched astrocytes. We found that p35 and glial fibrillary acidic protein (GFAP) were co-localized in primary cultured and acute isolated brain cells. Cdk5 could form an immunocomplex with p35 and its activity was shown in pure primary cultured astrocytes. p35 was upregulated in astrocytes injured by scratching, concomitantly with upregulation of Cdk5 kinase a,activity. Pretreatment of the scratched astrocytes with a Cdk5 inhibitor, roscovitine, could delay wound healing by inhibiting the reorganization of tubulin, GFAP, and the extension of hypertrophic processes. Moreover, overexpression of dominant negative Cdk5 could shorten the length of extending protrusion of reactive astrocytes. Thus, our findings demons rated that functional Cdk5, associated with p35, was expressed in astrocytes and its activity could be upregulated in reactive astrocytes, a new role of Cdk5 that has never been reported in the nervous system. The present study may provide new insight for understanding the multifunctional protein complex Cdk5/p35 in the nervous system. (c) 2007 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000244799000002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68130
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: Peking Univ, Neurosci Res Inst, Dept Neurobiol, Key Lab Neurosci, Beijing 100083, Peoples R China

Recommended Citation:
He, Yi,Li, Hui-Li,Xie, Wei-Yan,et al. The presence of active Cdk5 associated with p35 in astrocytes and its important role in process elongation of scratched astrocyte[J]. GLIA,2007,55(6):573-583.
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