IR@PKUHSC  > 北京大学基础医学院  > 神经生物学系
学科主题基础医学
The presence of active Cdk5 associated with p35 in astrocytes and its important role in process elongation of scratched astrocyte
He, Yi; Li, Hui-Li; Xie, Wei-Yan; Yang, Chun-Zhang; Yu, Albert Cheung Hoi; Wang, Yun
关键词Cdk5/p35 Astrocyte Injury Hypertrophy Scratch
刊名GLIA
2007-04-15
DOI10.1002/glia.20485
55期:6页:573-583
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]CYCLIN-DEPENDENT KINASE-5 ; REACTIVE ASTROCYTES ; NERVOUS-SYSTEM ; GROWTH-FACTOR ; REGULATORY SUBUNIT ; CELL ; EXPRESSION ; INJURY ; DIFFERENTIATION ; MIGRATION
英文摘要

Cyclin-dependent kinase 5 (Cdk5) is a unique member of the Cdk family its kinase activity requires association with its activator, p35 or p39. p35 is the strongest and best characterized activator. Previous studies showed that p35 is a neuron-specific protein that restricts Cdk5 activity in neurons. However, a high expression level of Cdk5 is found in astrocytes, which raises the possibility that astrocytic Cdk5 is functional. Here we show the presence of functional Cdk5 associated with p35 in astrocytes and demonstrate its important role in process elongation of scratched astrocytes. We found that p35 and glial fibrillary acidic protein (GFAP) were co-localized in primary cultured and acute isolated brain cells. Cdk5 could form an immunocomplex with p35 and its activity was shown in pure primary cultured astrocytes. p35 was upregulated in astrocytes injured by scratching, concomitantly with upregulation of Cdk5 kinase a,activity. Pretreatment of the scratched astrocytes with a Cdk5 inhibitor, roscovitine, could delay wound healing by inhibiting the reorganization of tubulin, GFAP, and the extension of hypertrophic processes. Moreover, overexpression of dominant negative Cdk5 could shorten the length of extending protrusion of reactive astrocytes. Thus, our findings demons rated that functional Cdk5, associated with p35, was expressed in astrocytes and its activity could be upregulated in reactive astrocytes, a new role of Cdk5 that has never been reported in the nervous system. The present study may provide new insight for understanding the multifunctional protein complex Cdk5/p35 in the nervous system. (c) 2007 Wiley-Liss, Inc.

语种英语
WOS记录号WOS:000244799000002
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68130
专题北京大学基础医学院_神经生物学系
作者单位Peking Univ, Neurosci Res Inst, Dept Neurobiol, Key Lab Neurosci, Beijing 100083, Peoples R China
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He, Yi,Li, Hui-Li,Xie, Wei-Yan,et al. The presence of active Cdk5 associated with p35 in astrocytes and its important role in process elongation of scratched astrocyte[J]. GLIA,2007,55(6):573-583.
APA He, Yi,Li, Hui-Li,Xie, Wei-Yan,Yang, Chun-Zhang,Yu, Albert Cheung Hoi,&Wang, Yun.(2007).The presence of active Cdk5 associated with p35 in astrocytes and its important role in process elongation of scratched astrocyte.GLIA,55(6),573-583.
MLA He, Yi,et al."The presence of active Cdk5 associated with p35 in astrocytes and its important role in process elongation of scratched astrocyte".GLIA 55.6(2007):573-583.
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