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学科主题: 基础医学
题名:
Human SBK1 is dysregulated in multiple cancers and promotes survival of ovary cancer SK-OV-3 cells
作者: Wang, Pingzhang1,2,3; Guo, Jinhai1,2,3; Wang, Feng1; Shi, Taiping1,2,3; Ma, Dalong1,2,3
关键词: SH3-binding domain kinase 1 ; SBK1 ; Cancer ; Ovarian cancer ; Gene expression
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2011-06-01
DOI: 10.1007/s11033-010-0465-8
卷: 38, 期:5, 页:3551-3559
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: KINASE MUTATIONS ; GENE-EXPRESSION ; HUMAN GENOME ; DISEASE ; CLONING ; MODEL ; IDENTIFICATION ; KINMUTBASE ; PROTEINS ; DATABASE
英文摘要:

Protein kinases are involved in comprehensive cellular processes and also implicated in many human diseases. SH3-binding domain kinase 1 (SBK1) was first cloned and characterized in rat and the human cDNA was cloned in our lab in 2006, but the expression and function of endogenous protein have not been well studied in human. In this follow up study, we screened a panel of cell lines and tissues, as well as a tumor tissue array for SBK1 expression at both RNA and protein levels. To detect the protein, we generated the first rabbit polyclonal antibody against human SBK1. We show that the SBK1 is expressed in most of the cells and tissues examined, and the protein is highly up-regulated in ovarian serous adenocarcinoma while down-regulated in esophagus squamous cell carcinoma and stomach adenocarcinoma. When over-expressed in an ovarian cancer cells SK-OV-3 by adenovirus infection, SBK1 protected the cells from apoptosis induced by the viral infection, therefore promoting cancer cell survival. Given that a missense mutation K92E in human SBK1 was identified recently from ovarian mucinous carcinoma, together, these results suggest that the wide-spread expression pattern of human SBK1 may predict a broad cellular function, and its dysregulated in certain cancers suggests an involvement of the protein in the pathogenesis of human cancers.

语种: 英语
所属项目编号: 30600549
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000290102100087
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68131
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Pingzhang,Guo, Jinhai,Wang, Feng,et al. Human SBK1 is dysregulated in multiple cancers and promotes survival of ovary cancer SK-OV-3 cells[J]. MOLECULAR BIOLOGY REPORTS,2011,38(5):3551-3559.
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