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学科主题: 临床医学
题名:
A microfluidic system enabling continuous characterization of specific membrane capacitance and cytoplasm conductivity of single cells in suspension
作者: Zhao, Yang1; Chen, Deyong1; Li, Hao1; Luo, Yana1; Deng, Bin1; Huang, Song-Bin2; Chiu, Tzu-Keng2; Wu, Min-Hsien2; Long, Rong3; Hu, Hao4; Wang, Junbo1; Chen, Jian1
关键词: Microfluidics ; Cellular biophysics ; Single cell analysis ; Specific membrane capacitance ; Cytoplasm conductivity ; Impedance spectroscopy
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2013-05-15
DOI: 10.1016/j.bios.2012.12.035
卷: 43, 页:304-307
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]: IMPEDANCE CYTOMETRY ; BLOOD
英文摘要:

This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (C-specific membrane) and cytoplasm conductivity (sigma(cytoplasm)) of single cells in suspension. In this study, cells were aspirated continuously through a constriction channel while cell elongations and impedance profiles at two frequencies (1 kHz and 100 kHz) were measured simultaneously using microscopy imaging and a lock-in amplifier. 1 kHz impedance data were used to evaluate cellular sealing properties with constriction channel walls and 100 kHz impedance data were translated to quantify equivalent membrane capacitance and cytoplasm resistance of single cells, which were further translated to C-specific membrane and sigma(cytoplasm). Two model cell lines (kidney tumor cell line of 786-O (n = 302) and vascular smooth muscle cell line of T2 (n = 216)) were used to evaluate this technique, producing C-specific membrane of 3.67 +/- 1.00 and 4.53 +/- 1.51 mu F/cm(2) and sigma(cytoplasm) of 0.47 +/- 0.09 and 0.55 +/- 0.14 S/m, respectively. Compared to previously reported techniques which can only collect C-specific membrane and sigma(cytoplasm) from tens of cells, this new technique has a higher throughput, capable of collecting C-specific membrane and sigma(cytoplasm) from hundreds of cells in 30 min immediately after cell passage. (c) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 61201077 ; 81261120561 ; PIRSES-GA-2009-247641
项目资助者: NSFC (National Natural Science Foundation of China) ; FP7
WOS记录号: WOS:000316525300051
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68141
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Chang Gung Univ, Inst Biochem & Biomed Engn, Tao Yuan, Taiwan
2.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing, Peoples R China
3.Univ Colorado, Dept Mech Engn, Boulder, CO 80309 USA
4.Peking Univ, Hlth Sci Ctr, Peoples Hosp, Dept Urol, Beijing 100871, Peoples R China

Recommended Citation:
Zhao, Yang,Chen, Deyong,Li, Hao,et al. A microfluidic system enabling continuous characterization of specific membrane capacitance and cytoplasm conductivity of single cells in suspension[J]. BIOSENSORS & BIOELECTRONICS,2013,43:304-307.
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