IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China
Wang, YC; Zhang, HY; Xia, NS; Peng, G; Lan, HY; Zhuang, H; Zhu, YH; Li, SW; Tian, KG; Gu, WJ; Lin, JX; Wu, X; Li, HM; Harrison, TJ
关键词genotype RT-PCR enzyme immunoassay zoonosis
刊名JOURNAL OF MEDICAL VIROLOGY
2002-08-01
DOI10.1021/jmv.10131
67期:4页:516-521
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]UNITED-STATES ; SWINE ; INFECTION ; STRAINS ; IDENTIFICATION ; GENOTYPE ; TAIWAN ; HEV
英文摘要

Evidence that hepatitis E is zoonotic is accumulating. Serum samples were collected from pigs, cattle, and goats from various regions of China to determine whether they had been infected with hepatitis E virus (HEV). An in-house enzyme immunoassay (EIA) and reverse transcriptase-polymerase chain reaction (RT-PCR) with primers from open reading frame (ORF) 2 were used to detect anti-HEV antibodies and HEV RNA. The mean positivity rates of anti-HEV antibody for pigs and cattle were 78.8% and 6.3% but none of the goat sera were positive. Pigs may be more susceptible to infection with HEV than cattle or goats. Five of 263 pig sera were positive for HEV RNA and four of these five were also positive for anti-HEV. The PCR products (nt 6007-6354) were cloned and sequenced and compared to other HEV sequences in the nucleotide databases. The five sequences shared 83-93% identity to each other at the nucleotide level and 74-79%, 73-74%, 73-78%, and 83-99% identity to HEV genotypes 1, 2, 3, and 4, respectively. They were closely related to human isolates of HEV genotype 4. Phylogenetic analyses also place these swine sequences in HEV genotype 4, resembling most closely viruses isolated from Chinese patients with acute hepatitis. These data support the hypothesis that sporadic hepatitis E in China is zoonotic. (C) 2002 Wiley-Liss, Inc.

语种英语
WOS记录号WOS:000176589900008
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被引频次:122[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/68143
专题北京大学基础医学院_病原生物学系
作者单位1.UCL, Ctr Hepatol, Royal Free & Univ Coll Med Sch, London NW3 2PF, England
2.Natl Inst Control Pharmaceut Biol Prod, Beijing, Peoples R China
3.UCL, Royal Free & Univ Coll, Ctr Hepatol, London, England
4.Xiamen Univ, Dept Biol, Xiamen, Fujian Province, Peoples R China
5.Wantai Biopharmaceut Co, Beijing, Peoples R China
6.Beijing Univ, Div Med, Dept Microbiol, Beijing, Peoples R China
7.Wantai Biopharmaceut Co, Beijing, Peoples R China
8.Beijing Univ, Dept Microbiol, Div Med, Beijing 100871, Peoples R China
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GB/T 7714
Wang, YC,Zhang, HY,Xia, NS,et al. Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China[J]. JOURNAL OF MEDICAL VIROLOGY,2002,67(4):516-521.
APA Wang, YC.,Zhang, HY.,Xia, NS.,Peng, G.,Lan, HY.,...&Harrison, TJ.(2002).Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China.JOURNAL OF MEDICAL VIROLOGY,67(4),516-521.
MLA Wang, YC,et al."Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China".JOURNAL OF MEDICAL VIROLOGY 67.4(2002):516-521.
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