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学科主题: 基础医学
题名:
Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China
作者: Wang, YC; Zhang, HY; Xia, NS; Peng, G; Lan, HY; Zhuang, H; Zhu, YH; Li, SW; Tian, KG; Gu, WJ; Lin, JX; Wu, X; Li, HM; Harrison, TJ
关键词: genotype ; RT-PCR ; enzyme immunoassay ; zoonosis
刊名: JOURNAL OF MEDICAL VIROLOGY
发表日期: 2002-08-01
DOI: 10.1021/jmv.10131
卷: 67, 期:4, 页:516-521
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: UNITED-STATES ; SWINE ; INFECTION ; STRAINS ; IDENTIFICATION ; GENOTYPE ; TAIWAN ; HEV
英文摘要:

Evidence that hepatitis E is zoonotic is accumulating. Serum samples were collected from pigs, cattle, and goats from various regions of China to determine whether they had been infected with hepatitis E virus (HEV). An in-house enzyme immunoassay (EIA) and reverse transcriptase-polymerase chain reaction (RT-PCR) with primers from open reading frame (ORF) 2 were used to detect anti-HEV antibodies and HEV RNA. The mean positivity rates of anti-HEV antibody for pigs and cattle were 78.8% and 6.3% but none of the goat sera were positive. Pigs may be more susceptible to infection with HEV than cattle or goats. Five of 263 pig sera were positive for HEV RNA and four of these five were also positive for anti-HEV. The PCR products (nt 6007-6354) were cloned and sequenced and compared to other HEV sequences in the nucleotide databases. The five sequences shared 83-93% identity to each other at the nucleotide level and 74-79%, 73-74%, 73-78%, and 83-99% identity to HEV genotypes 1, 2, 3, and 4, respectively. They were closely related to human isolates of HEV genotype 4. Phylogenetic analyses also place these swine sequences in HEV genotype 4, resembling most closely viruses isolated from Chinese patients with acute hepatitis. These data support the hypothesis that sporadic hepatitis E in China is zoonotic. (C) 2002 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000176589900008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68143
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.UCL, Ctr Hepatol, Royal Free & Univ Coll Med Sch, London NW3 2PF, England
2.Natl Inst Control Pharmaceut Biol Prod, Beijing, Peoples R China
3.UCL, Royal Free & Univ Coll, Ctr Hepatol, London, England
4.Xiamen Univ, Dept Biol, Xiamen, Fujian Province, Peoples R China
5.Wantai Biopharmaceut Co, Beijing, Peoples R China
6.Beijing Univ, Div Med, Dept Microbiol, Beijing, Peoples R China
7.Wantai Biopharmaceut Co, Beijing, Peoples R China
8.Beijing Univ, Dept Microbiol, Div Med, Beijing 100871, Peoples R China

Recommended Citation:
Wang, YC,Zhang, HY,Xia, NS,et al. Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China[J]. JOURNAL OF MEDICAL VIROLOGY,2002,67(4):516-521.
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