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学科主题: 临床医学
题名:
Apeliin activates L-arginine/nitric oxide synthase/nitric oxide pathway in rat aortas
作者: Iia, Yue Xia; Lu, Zheng Fei; Zhang, Jing; Pan, Chun Shui; Yang, Jing Hui; Zhao, Jing; Yu, Fang; Duan, Xiao Hui; Tang, Chao Shu; Qi, Yong Fen
关键词: apelin nitric oxide ; nitric oxide synthase ; cationic amino acid transporters ; L-arginine
刊名: PEPTIDES
发表日期: 2007-10-01
DOI: 10.1016/j.peptides.2007.07.016
卷: 28, 期:10, 页:2023-2029
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: AMINO-ACID TRANSPORTER ; SMOOTH-MUSCLE-CELLS ; NITRIC-OXIDE ; GENE-EXPRESSION ; APJ RECEPTOR ; VASCULAR ENDOTHELIUM ; TISSUE DISTRIBUTION ; ENDOGENOUS LIGAND ; IN-VIVO ; ADRENOMEDULLIN
英文摘要:

Apelin was recently found to be an inotropic polypeptide in isolated rat hearts, and intravenous injection of apelin can induce a transient decrease in blood pressure. To illustrate the mechanism of apelin-induced vasodilation, we observed the in vitro effects of apelin on the L-arginine (L-Arg)/nitric oxide (NO) pathway in the incubated, isolated rat aorta. Apelin stimulated vascular NO2- product and NOS activation in a concentration- and time-dependent manner. Compared with no apelin treatment, incubation with apelin (10(-9), 10(-8), and 10(-7) mol/L) increased NO2- product by 33%, 46%, and 69% (all p < 0.01), respectively, and Ca2+-dependent constitutive NOS (cNOS) activity by 200%, 460%, and 550% (all p < 0.01), respectively. However, Ca2+-independent NOS (iNOS) activity was not significantly altered (p > 0.05). Apelin incubation (10(-9), 10(-8), and 10(-7) mol/L) increased L-Arg uptake by 130%, 180%, and 240% (all p < 0.01), respectively. The mRNA level of cationic amino acid transporters, CAT-1 and CAT-2B, in rat aortic tissues treated with 10(-7) mol/L apelin was increased by 110% and 128%, respectively (both p < 0.01). Incubation with 10(-7) mol/L apelin elevated eNOS rnRNA and protein levels, by 53% (p < 0.05) and 319% (p < 0.01), respectively. Collectively, these results demonstrate that apelin directly activated the vascular L-Arg/ NOS/NO pathway, which could be one of the important mechanisms of apelin-regulated vascular function. (C) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000250326100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68187
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
4.Beijing Normal Univ, Sch PE & Sports Sci, Beijing 100875, Peoples R China

Recommended Citation:
Iia, Yue Xia,Lu, Zheng Fei,Zhang, Jing,et al. Apeliin activates L-arginine/nitric oxide synthase/nitric oxide pathway in rat aortas[J]. PEPTIDES,2007,28(10):2023-2029.
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