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学科主题: 临床医学
题名:
Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
作者: Yang, Fan; Wang, Dongmei; Wu, Lingling; Li, Ying
关键词: glaucoma ; triptolide ; microglia ; neuroprotection
刊名: DRUG DESIGN DEVELOPMENT AND THERAPY
发表日期: 2015
DOI: 10.2147/DDDT.S92022
卷: 9, 页:6095-6107
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: NECROSIS-FACTOR-ALPHA ; INFLAMMATION-MEDIATED DAMAGE ; DBA/2J MOUSE MODEL ; OPTIC-NERVE HEAD ; TNF-ALPHA ; MICROGLIAL ACTIVATION ; DOPAMINERGIC-NEURONS ; RESPONSES ; NEURODEGENERATION ; RECEPTOR-1
英文摘要:

Purpose: To study the effects of triptolide, a Chinese herb extract, on retinal ganglion cells (RGCs) in a rat model of chronic glaucoma.

Methods: Eighty Wistar rats were randomly divided into triptolide group (n = 40) and normal saline (NS) group (n = 40). Angle photocoagulation was used to establish the model of glaucoma, with right eye as laser treated eye and left eye as control eye. Triptolide group received triptolide intraperitoneally daily, while NS group received NS. Intraocular pressure (IOP), anti-CD11b immunofluorescent stain in retina and optic nerve, RGCs count with Nissel stain and microglia count with anti-CD11b immunofluorescence stain in retina flat mounts, retinal tumor necrosis factor (TNF)-alpha mRNA detection by reverse transcription-polymerase chain reaction, and double immunofluorescent labeling with anti-TNF-alpha and anti-CD11b in retinal frozen section were performed.

Results: Mean IOP of the laser treated eyes significantly increased 3 weeks after photocoagulation (P<0.05), with no statistical difference between the two groups (P<0.05). RGCs survival in the laser treated eyes was significantly improved in the triptolide group than the NS group (P<0.05). Microglia count in superficial retina of the laser treated eyes was significantly less in the triptolide group (30.40 +/- 4.90) than the NS group (35.06 +/- 7.59) (P<0.05). TNF-alpha mRNA expression in the retina of the laser treated eyes in the triptolide group decreased by 60% compared with that in the NS group (P<0.01). The double immunofluorescent labeling showed that TNF-alpha was mainly distributed around the microglia.

Conclusion: Triptolide improved RGCs survival in this rat model of chronic glaucoma, which did not depend on IOP decrease but might be exerted by inhibiting microglia activities and reducing TNF-alpha secretion.

语种: 英语
WOS记录号: WOS:000366022400001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68201
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: Peking Univ, Dept Ophthalmol, Hosp 3, Beijing 100871, Peoples R China

Recommended Citation:
Yang, Fan,Wang, Dongmei,Wu, Lingling,et al. Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma[J]. DRUG DESIGN DEVELOPMENT AND THERAPY,2015,9:6095-6107.
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