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学科主题: 基础医学
题名:
Identification of a new HLA-A*0201-restricted CD8(+) T cell epitope from hepatocellular carcinoma-associated antigen HCA587
作者: Li, B; Wang, Y; Chen, J; Wu, H; Chen, W
关键词: cancer/testis antigen ; ELISPOT ; heptocellular carcinoma ; immunotherapy
刊名: CLINICAL AND EXPERIMENTAL IMMUNOLOGY
发表日期: 2005-05-01
DOI: 10.1111/j.1365-2249.2005.02786.x
卷: 140, 期:2, 页:310-319
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: CLASS-I MOLECULES ; DENDRITIC CELLS ; LYMPHOCYTE RESPONSES ; TUMOR-ERADICATION ; MELANOMA PATIENTS ; PROSTATE-CANCER ; MAGE FAMILY ; PEPTIDE ; VACCINATION ; EXPRESSION
英文摘要:

For the development of peptide-based cancer immunotherapies, we aimed to identify specific HLA-A*0201-restricted CTL epitopes in hepatocellular carcinoma (HCC) associated antigen HCA587, which has been identified as a member of the cancer/testis (CT) antigens highly expressed in HCC. We first combined the use of an HLA-A*0201/peptide binding algorithm and T2 binding assays with the induction of specific CD8(+) T cell lines from normal donors by in vitro priming with high-affinity peptides, then IFN-gamma release and cytotoxicity assays were employed to identify the specific HLA-A*0201 CD8(+) T cell epitope using peptide-loaded T2 cells or the HCA587 protein(+) HCC cell line HepG2. In the six candidate synthesized peptides, two peptides showed higher binding ability in T2 binding assays. No. 2 peptide, encompassing amino acid residues FLAKLNNTV (HCA587(317-325)), was able to activate a HCA587-specific CD8(+) T-cell response in human lymphocyte cultures from two normal donors and two HCC patients, and these HCA587-specific CD8(+) T cells recognized peptide-pulsed T2 cells as well as the HCA587 protein(+) HCC cell line HepG2 in IFN-gamma release and cytotoxicity assays. The results indicate that no. 2 peptide is a new HLA-A*0201-restricted CTL epitope capable of inducing HCA587-specific CTLs. Our data suggest that identification of this new HCA587/HLA-A*0201 peptide FLAKLNNTV may facilitate the design of peptide-based immunotherapies for the treatment of HCA587-bearing HCC patients.

语种: 英语
WOS记录号: WOS:000227968900015
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68209
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Li, B,Wang, Y,Chen, J,et al. Identification of a new HLA-A*0201-restricted CD8(+) T cell epitope from hepatocellular carcinoma-associated antigen HCA587[J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY,2005,140(2):310-319.
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