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学科主题: 药学
题名:
Simultaneous enantiomer determination of 20 (R)- and 20 (S)-ginsenoside-Rg(2) in rat plasma after intravenous administration using HPLC method
作者: Gui, Fang-Jin; Yang, Xiu-Wei; Li, Long-Yun; Tian, Jian-Ming
关键词: 20 (R,S)-ginsenoside-Rg(2) ; 20 (R)-ginsenoside-Rg(2) ; 20 (S)-Ginsenoside-Rg(2) ; enantiomeric separation ; pharmacokinetics
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007-05-01
DOI: 10.1016/j.jchromb.2006.11.008
卷: 850, 期:1-2, 页:1-6
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; STATIONARY-PHASE ; SEPARATION ; METABOLITES ; GINSENG ; COLUMN ; URINE
英文摘要:

20 (R,S)-Ginsenoside-Rg(2,) an anti-shock agent, is prescribed as a racemate. To asnaslyze simultaneously the enantiomers of 20 (R)-ginsenoside-Rg(2) and 20 (S)-ginsenoside-Rg(2) in plasma, a simple and reproducible high-performance liquid chromatographic (HPLC) method has been developed. The enantiomeric separation and determination were successefully achieved using a Diamonsil (TM) ODS C-18 reversed-phase column (5 mu m, 250 mm x 4.6 mm) with an RP18 (5 mu m) guard column and a mobile phase of MeOH-aq. 4% H3PO4 (65:35, v/v, pH 5.1) with UV detection at 203 nm. Both enantiometers, 20 (R)-ginsenoside-Rg(2) and 20 (S)-ginsenoside-Rg(2), were well separated at 14.5 min and 13.6 min, respectively. The linear ranges of the standard curves were 2.0-250 mu g/ml. The intra- and inter-day precision (R.S.D.) were <= 1.59% and <= 0.54% and the mean extraction recoveries were 95.8% and 96.5% for 20 (R)-ginsenoside-Rg(2) and 20 (S)-ginsenoside-Rg(2) in rat plasma, respectively. The limits of detection and quantification were 2.0 mu g/ml and 7.8 mu g/ml (S/N = 3:1) for 20 (R)-ginsenoside-Rg(2), and 20 mu g/ml and 3.9 mu g/ml (S/N = 3:1) for 20 (S)-ginsenoside Rg(2), respectively. This validated method was applicable to pharmacokinetic studies in rat plasma after intravenous administration of 20 (R,S)-ginsenoside-Rg(2). A pharmacokinetic study in rat plasma indicated that the enantiomers were rapidly absorbed and eliminated. These assays results are necessary for he evaluation of the metabolism of ginsenoside-Rg(2) in vivo. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000246703500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68217
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100080, Peoples R China
2.Peking Univ, Med & Hlth Anal Ctr, Beijing 100080, Peoples R China
3.Jilin Acad Tradit Chinese Med, Changchun 130021, Peoples R China

Recommended Citation:
Gui, Fang-Jin,Yang, Xiu-Wei,Li, Long-Yun,et al. Simultaneous enantiomer determination of 20 (R)- and 20 (S)-ginsenoside-Rg(2) in rat plasma after intravenous administration using HPLC method[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,850(1-2):1-6.
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