IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
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Epidemiological Characteristics of bla(NDM-1) in Enterobacteriaceae and the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in China from 2011 to 2012
Ou, Weimei; Cui, Lanqing; Li, Yun; Zheng, Bo; Lv, Yuan
刊名PLOS ONE
2014-12-03
DOI10.1371/journal.pone.0113852
9期:12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]EMERGENCE ; NDM-1 ; IDENTIFICATION ; COEXISTENCE ; PATIENT ; TAIWAN ; INDIA
英文摘要

Objectives: The study aimed to investigate the prevalence and epidemiological characteristics of bla(NDM-1) (encoding New Delhi metallo-b-lactamase 1) in Enterobacteriaceae and the Acinetobacter calcoaceticus-Acinetobacter baumannii complex (ABC) in China from July 2011 to June 2012.

Methods: PCR was used to screen for the presence of bla(NDM-1) in all organisms studied. For bla(NDM-1)-positive strains, 16S rRNA analysis and Analytical Profile Index (API) strips were used to identify the bacterial genus and species. The ABCs were reconfirmed by PCR detection of bla(OXA-51-like). Antibiotic susceptibilities of the bacteria were assessed by determining minimum inhibitory concentration (MIC) of them using two-fold agar dilution test, as recommended by the Clinical and Laboratory Standards Institute (CLSI). Molecular typing was performed using pulsed-field gel electrophoresis (PFGE). S1 nuclease-pulsed-field gel electrophoresis (S1-PFGE) and Southern blot hybridization were conducted to ascertain the gene location of bla(NDM-1). Conjugation experiments were conducted to determine the transmission of bla(NDM-1)-positive strains.

Results: Among 2,170 Enterobacteriaceae and 600 ABCs, seven Enterobacteriaceae strains and two A. calcoaceticus isolates from five different cities carried the bla(NDM-1) gene. The seven Enterobacteriaceae strains comprised four Klebsiella pneumoniae, one Enterobacter cloacae, one Enterobacter aerogen and one Citrobacter freundii. All seven were non-susceptible to imipenem, meropenem or ertapenem. Two A. calcoaceticus species were resistant to imipenem and meropenem. Three K. pneumoniae showed the same PFGE profiles. The bla(NDM-1) genes of eight strains were localized on plasmids, while one was chromosomal.

Conclusions: Compared with previous reports, the numbers and species containing the bla(NDM-1) in Enterobacteriaceae have significantly increased in China. Most of them are able to disseminate the gene, which is cause for concern. Consecutive surveillance should be implemented and should also focus on the dissemination of bla(NDM-1) among gram-negative clinical isolates.

语种英语
WOS记录号WOS:000349128700048
项目编号2012ZX09303005-003
资助机构National Science and Technology Major Project
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68225
专题北京大学第一临床医学院_临床药理研究所
作者单位Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ou, Weimei,Cui, Lanqing,Li, Yun,et al. Epidemiological Characteristics of bla(NDM-1) in Enterobacteriaceae and the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in China from 2011 to 2012[J]. PLOS ONE,2014,9(12).
APA Ou, Weimei,Cui, Lanqing,Li, Yun,Zheng, Bo,&Lv, Yuan.(2014).Epidemiological Characteristics of bla(NDM-1) in Enterobacteriaceae and the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in China from 2011 to 2012.PLOS ONE,9(12).
MLA Ou, Weimei,et al."Epidemiological Characteristics of bla(NDM-1) in Enterobacteriaceae and the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in China from 2011 to 2012".PLOS ONE 9.12(2014).
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