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学科主题: 临床医学
题名:
Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells
作者: Yan, Liying1,2,3; Yang, Mingyu1,2; Guo, Hongshan1,2; Yang, Lu1,2; Wu, Jun1,2; Li, Rong1,2,3; Liu, Ping1,2; Lian, Ying1,2; Zheng, Xiaoying1,2; Yan, Jie1,2; Huang, Jin1,2; Li, Ming1,2; Wu, Xinglong1,2; Wen, Lu1,2; Lao, Kaiqin4; Li, Ruiqiang1,2,5; Qiao, Jie1,2,3; Tang, Fuchou1,2
刊名: NATURE STRUCTURAL & MOLECULAR BIOLOGY
发表日期: 2013-09-01
DOI: 10.1038/nsmb.2660
卷: 20, 期:9, 页:1131-+
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: LONG NONCODING RNAS ; GENE-EXPRESSION PROFILES ; TRANSCRIPTOME ANALYSIS ; HUMAN TROPHECTODERM ; BLASTOCYST ; GENOME ; MOUSE ; PLURIPOTENCY ; DERIVATION ; ALIGNMENT
英文摘要:

Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. Here we apply single-cell RNA sequencing (RNA-Seq) analysis to 124 individual cells from human preimplantation embryos and human embryonic stem cells (hESCs) at different passages. The number of maternally expressed genes detected in our data set is 22,687, including 8,701 long noncoding RNAs (lncRNAs), which represents a significant increase from 9,735 maternal genes detected previously by cDNA microarray. We discovered 2,733 novel lncRNAs, many of which are expressed in specific developmental stages. To address the long-standing question whether gene expression signatures of human epiblast (EPI) and in vitro hESCs are the same, we found that EPI cells and primary hESC outgrowth have dramatically different transcriptomes, with 1,498 genes showing differential expression between them. This work provides a comprehensive framework of the transcriptome landscapes of human early embryos and hESCs.

语种: 英语
所属项目编号: 2012CB966704 ; 2011CB966303 ; 2011CB944500 ; 31271543 ; 30825038 ; 81000275
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Funds for Distinguished Young Scholar ; National Science Foundation of China
WOS记录号: WOS:000324160900018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68231
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Coll Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Coll Life Sci, Ctr Reprod Med, Beijing 100871, Peoples R China
3.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
4.Life Technol, Appl Biosyst, Genet Syst, Foster City, CA USA
5.Peking Univ, Coll Life Sci, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China

Recommended Citation:
Yan, Liying,Yang, Mingyu,Guo, Hongshan,et al. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2013,20(9):1131-+.
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