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IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科  > 期刊论文
学科主题: 临床医学
题名:
Expression of subtypes of somatostatin receptors in hepatic stellate cells
作者: Song, Sheng-Han; Leng, Xi-Sheng; Li, Tao; Qin, Zhi-Zhong; Peng, Ji-Run; Zhao, Li; Wei, Yu-Hua; Yu, Xin
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2004-06-01
卷: 10, 期:11, 页:1663-1665
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: To elucidate the mechanism by which somatostatin and its analogue exert the influence on liver fibrosis, and to investigate the mRNA expression of somatostatin receptors subtypes (SSTRs) and the distribution of somatostatin analogue octreotide in rat hepatic stellate cells (HSCs).

METHODS: HSCs were isolated from Sprague Dawley (SD) rats by in situ perfusion and density gradient centrifugation. After several passages, the mRNA expression of 5 subtypes of SSTRs were assessed by reverse transcription-polymerase chain reaction (RT-PCR). HSCs were planted on coverslip and co-cultured with octreotide tagged by FITC. Then the distribution of FITC fluorescence was observed under laser scanning confocal microscope (LSCM) in 12-24 h.

RESULTS: There were mRNA expression of SSTR2, SSTR3 and SSTR5 but not SSTR1 and SSTR4 in SD rat HSCs. The mRNA expression level of SSTR2 was significantly higher than that of other subtypes (P<0.01). FITC fluorescence of octreotide was clearly observed on the surface and in the cytoplasm, but not in the nuclei of HSCs under LSCM.

CONCLUSION: The effect exerted by somatostatin and its analogues on HSCs may mainly depend on the expression of SSTR2, SSTR3 and SSTR5. Octreotide can perfectly combine with HSCs, and thereby exerts its biological activity on regulating the characters of active HSCs. This provides a potential prevention and management against liver fibrosis.

语种: 英语
所属项目编号: 30271270
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000208097000027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68235
Appears in Collections:北京大学第二临床医学院_肝胆外科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Hepatobiliary Surg, Beijing 100044, Peoples R China

Recommended Citation:
Song, Sheng-Han,Leng, Xi-Sheng,Li, Tao,et al. Expression of subtypes of somatostatin receptors in hepatic stellate cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(11):1663-1665.
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