IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科
学科主题临床医学
Expression of subtypes of somatostatin receptors in hepatic stellate cells
Song, Sheng-Han; Leng, Xi-Sheng; Li, Tao; Qin, Zhi-Zhong; Peng, Ji-Run; Zhao, Li; Wei, Yu-Hua; Yu, Xin
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2004-06-01
10期:11页:1663-1665
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
英文摘要

AIM: To elucidate the mechanism by which somatostatin and its analogue exert the influence on liver fibrosis, and to investigate the mRNA expression of somatostatin receptors subtypes (SSTRs) and the distribution of somatostatin analogue octreotide in rat hepatic stellate cells (HSCs).

METHODS: HSCs were isolated from Sprague Dawley (SD) rats by in situ perfusion and density gradient centrifugation. After several passages, the mRNA expression of 5 subtypes of SSTRs were assessed by reverse transcription-polymerase chain reaction (RT-PCR). HSCs were planted on coverslip and co-cultured with octreotide tagged by FITC. Then the distribution of FITC fluorescence was observed under laser scanning confocal microscope (LSCM) in 12-24 h.

RESULTS: There were mRNA expression of SSTR2, SSTR3 and SSTR5 but not SSTR1 and SSTR4 in SD rat HSCs. The mRNA expression level of SSTR2 was significantly higher than that of other subtypes (P<0.01). FITC fluorescence of octreotide was clearly observed on the surface and in the cytoplasm, but not in the nuclei of HSCs under LSCM.

CONCLUSION: The effect exerted by somatostatin and its analogues on HSCs may mainly depend on the expression of SSTR2, SSTR3 and SSTR5. Octreotide can perfectly combine with HSCs, and thereby exerts its biological activity on regulating the characters of active HSCs. This provides a potential prevention and management against liver fibrosis.

语种英语
WOS记录号WOS:000208097000027
项目编号30271270
资助机构National Nature Science Foundation of China
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68235
专题北京大学第二临床医学院_肝胆外科
北京大学精神卫生研究所_精神科
作者单位Peking Univ, Peoples Hosp, Dept Hepatobiliary Surg, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Song, Sheng-Han,Leng, Xi-Sheng,Li, Tao,et al. Expression of subtypes of somatostatin receptors in hepatic stellate cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(11):1663-1665.
APA Song, Sheng-Han.,Leng, Xi-Sheng.,Li, Tao.,Qin, Zhi-Zhong.,Peng, Ji-Run.,...&Yu, Xin.(2004).Expression of subtypes of somatostatin receptors in hepatic stellate cells.WORLD JOURNAL OF GASTROENTEROLOGY,10(11),1663-1665.
MLA Song, Sheng-Han,et al."Expression of subtypes of somatostatin receptors in hepatic stellate cells".WORLD JOURNAL OF GASTROENTEROLOGY 10.11(2004):1663-1665.
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