IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Dyskerin Overexpression in Human Hepatocellular Carcinoma Is Associated with Advanced Clinical Stage and Poor Patient Prognosis
Liu, Bei1; Zhang, Jinglei2; Huang, Chen3; Liu, Hui2
刊名PLOS ONE
2012-08-13
DOI10.1371/journal.pone.0043147
7期:8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Ministry of Science and Technology, China ; Shanghai Municipal Health Bureau ; Special Program for Military Medicine, China ; Ministry of Science and Technology, China ; Shanghai Municipal Health Bureau ; Special Program for Military Medicine, China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]HUMAN BREAST-CANCER ; RIBOSOMAL-RNA ; TELOMERASE ACTIVITY ; EXPRESSION ; GENES ; DKC1 ; CARCINOGENESIS ; CLASSIFICATION ; ACCUMULATION ; TRANSLATION
英文摘要

Background: Dyskerin (encoded by the DKC1 gene) is an essential nucleolar protein involved in cell proliferation, where it is required for the pseudo-uridylation of ribosomal RNA (rRNA) molecules and the stabilization of the telomerase RNA component. Dyskerin expression has been reported to predict poor survival in some cancer patients. The aim of the present study was to analyze the expression of dyskerin in hepatocellular carcinoma (HCC) and to determine its correlation with clinicopathologic features, including the survival of patients with HCC.

Methodology/Principal Findings: Dyskerin protein expression was detected by immunohistochemistry in paraffin sections of 252 HCC cases and 80 noncancerous liver tissues. The correlation was analyzed between dyskerin expression levels and clinicopathologic variables and prognosis. Dyskerin protein was significantly overexpressed in HCC tissues when compared to noncancerous liver tissue. Dyskerin overexpression was positively correlated with the hepatitis B surface antigen status, serum alpha-fetoprotein, and advanced clinical stage in HCC patients. A survival analysis indicated that HCC patients with higher dyskerin expression had a significantly shorter overall survival and 5-year survival time when compared to those with low expression. A multivariate analysis suggested that dyskerin overexpression was an independent factor for prognosis (hazard risk, 2.912; P = 0.007). Expression of DKC1 mRNA was measured by quantitative RT-PCR in 80 HCC and 50 noncancerous tissues. The relationship between DKC1, TERT, MKI67, and MYC mRNA expression in HCC tissues was also evaluated. DKC1 mRNA was significantly overexpressed in HCC tissues and showed a significant correlation with MKI67 and MYC mRNA but a weak correlation with TERT mRNA.

Conclusions/Significance: Dyskerin overexpression in HCC patients was correlated with MYC and MKI67 expression and showed a possible involvement in the tumorigenic process. Dyskerin overexpression may be an unfavorable prognostic factor in patients with HCC.

语种英语
所属项目编号010S2012ZR0058 ; 2008Y077 ; 2010JS15
资助者Ministry of Science and Technology, China ; Shanghai Municipal Health Bureau ; Special Program for Military Medicine, China ; Ministry of Science and Technology, China ; Shanghai Municipal Health Bureau ; Special Program for Military Medicine, China
WOS记录号WOS:000307500100067
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68238
专题北京大学第三临床医学院
作者单位1.Wuhan Univ Sci & Technol, Affiliated Tianyou Hosp, Dept Pathol, Wuhan, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Shanghai, Peoples R China
3.Peking Univ, Hosp 3, Med Res Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Liu, Bei,Zhang, Jinglei,Huang, Chen,et al. Dyskerin Overexpression in Human Hepatocellular Carcinoma Is Associated with Advanced Clinical Stage and Poor Patient Prognosis[J]. PLOS ONE,2012,7(8).
APA Liu, Bei,Zhang, Jinglei,Huang, Chen,&Liu, Hui.(2012).Dyskerin Overexpression in Human Hepatocellular Carcinoma Is Associated with Advanced Clinical Stage and Poor Patient Prognosis.PLOS ONE,7(8).
MLA Liu, Bei,et al."Dyskerin Overexpression in Human Hepatocellular Carcinoma Is Associated with Advanced Clinical Stage and Poor Patient Prognosis".PLOS ONE 7.8(2012).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Bei]的文章
[Zhang, Jinglei]的文章
[Huang, Chen]的文章
百度学术
百度学术中相似的文章
[Liu, Bei]的文章
[Zhang, Jinglei]的文章
[Huang, Chen]的文章
必应学术
必应学术中相似的文章
[Liu, Bei]的文章
[Zhang, Jinglei]的文章
[Huang, Chen]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。