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学科主题: 临床医学
题名:
Hydrogen sulfide regulates lung tissue-oxidized glutathione and total antioxidant capacity in hypoxic pulmonary hypertensive rats
作者: Wei, Hong-ling1; Zhang, Chun-yu1; Jin, Hong-fang1; Tang, Chao-shu2,3; Du, Jun-bao1,2,3
关键词: hydrogen sulfide ; oxidative stress ; hypoxia pulmonary hypertension ; superoxide dismutase
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2008-06-01
DOI: 10.1111/j.1745-7254.2008.00796.x
卷: 29, 期:6, 页:670-676
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: SULFIDE/CYSTATHIONINE-GAMMA-LYASE ; SMOOTH-MUSCLE-CELLS ; OXIDATIVE STRESS ; BLOOD-FLOW ; PATHWAY ; VASOCONSTRICTION ; DISEASE ; IMPACT
英文摘要:

Aim: To investigate the modulatory effect of sudium hydrosulfide on lung tissue-oxidized glutathione and total antioxidant capacity in the development of hypoxic pulmonary hypertension (HPH). Methods: After 21 d of hypoxia, the mean pulmonary artery pressure was measured by cardiac catheterization. The plasma H(2)S level and production of H(2)S in the lung tissues were determined by using a spectrophotometer. The lung homogenates were assayed for total antioxidant capacity (T-AOC), superoxide dismutase (SOD), oxidized glutathione (GSSG), reduced glutathione and malonaldehyde by colorimetry. The mRNA level of SOD was analyzed by real-time PCR, and the SOD expression was detected by Western blotting. Results: In the hypoxia group, the plasma H(2)S concentration and H(2)S production in the lung was significantly decreased compared with the control group (187.2 +/- 13.1 vs 299.6 +/- 12.4 mu mol/L; 0.138 +/- 0.013 vs 0.289 +/- 0.036 nmol.mg(-1).min(-1), P < 0.01). The administration of sodium hydrosulfide could reduce the mean pulmonary artery pressure by 31.2% compared with the hypoxia group (P < 0.01). Treatment with sodium hydrosulfide decreased GSSG, and the T-AOC level of the lung tissues was enhanced compared with the hypoxia group (P < 0.05). There were no significant changes in the lung tissue SOD mRNA level, protein level, and its activity among the 3 groups. Conclusion: Oxidative stress occurred in the development of HPH and was accompanied by a decrease in the endogenous production of H(2)S in the lung tissues. H(2)S acted as an antioxidant during the oxidative stress of HPH partly as a result of the attenuated GSSG content.

语种: 英语
WOS记录号: WOS:000256441900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68247
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
3.Peking Univ, Key Lab Mol Cardiovasc Dis, Minist Educ, Beijing 100034, Peoples R China

Recommended Citation:
Wei, Hong-ling,Zhang, Chun-yu,Jin, Hong-fang,et al. Hydrogen sulfide regulates lung tissue-oxidized glutathione and total antioxidant capacity in hypoxic pulmonary hypertensive rats[J]. ACTA PHARMACOLOGICA SINICA,2008,29(6):670-676.
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