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学科主题临床医学
Effects and mechanisms of electroacupuncture at PC6 on frequency of transient lower esophageal sphincter relaxation in cats
Wang, Chi; Zhou, De-Feng; Shuai, Xiao-Wei; Liu, Jian-Xiang; Xie, Peng-Yan
关键词electroacupuncture transient lower esophageal sphincter relaxation C-fos nitric oxide cat
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2007-09-28
13期:36页:4873-4880
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]GASTROESOPHAGEAL-REFLUX ; GASTRIC DISTENSION ; NERVE-STIMULATION ; PROXIMAL STOMACH ; NITRIC-OXIDE ; ACUPUNCTURE ; ACHALASIA ; MOTILITY ; ENDORPHINS ; PRESSURE
英文摘要

AIM: To investigate the effects of electroacupuncture (EA) at neiguan (PC6) on gastric distention-induced transient lower esophageal sphincter relaxations (TLESRs) and discuss the mechanisms of this treatment.

METHODS: Protocol I : Twelve healthy cats underwent gastric distention for 60 min on the first day. Electrical acupoint stimulation was applied at the neiguan or a sham point on the hip in randomized order before gastric distention, on the third day and fifth day. Those cats that underwent EA at neiguan on the fifth day were named "Neiguan Group" and the cats that underwent EA at a sham acupoint on the fifth day were named "Sham Group" (control group). During the experiment the frequency of TLESRs and lower esophageal sphincter (LES) pressure were observed by a perfused sleeve assembly. Plasma levels of gastrin (GAS) and motilin (MTL) were determined by radioimmunoassay. Nitrite/nitrate concentration in plasma and tissues were measured by Griess reagent. The nuclei in the brain stem were observed by immunohistochemistry method of c-Fos and NADPH-d dyeing. Protocol II: Thirty six healthy cats were divided into 6 groups randomly. We gave saline (2 mL iv. control group), phaclofen (5 mg/kg iv. GABA-B antagonist), cholecystokinin octapeptide (CCK-8) (1 mu g/kg per hour iv.), L-Arginine (200 mg/kg iv.), naloxone (2.5 mu mol/kg iv.) and tacrine (5.6 mg/kg ip. cholinesterase inhibitor) respectively before EA at Neiguan and gastric distention. And the frequencies of TLESRs in experimental groups were compared with the control group.

RESULTS: Protocol I : Not only the frequency of gastric distention-incluced TLESR in 60 min but also the rate of common cavity during TLESRs were significantly decreased by EA at neiguan compared to that of sham acupoint stimulation. C-Fos immunoreactivity and NOS reactivity in the solitarius (NTS) and dorsal motor nucleus of the vagus (DMV) were significantly decreased by EA at neiguan compared to that of the sham group. However, the positive nuclei of C-Fos and NOS in reticular formation of the medulla (RFM) were increased by EA at neiguan. Protocol H: The inhibited effect of EA at neiguan on TLESRs frequency was completely restored by pretreatment with CCK (23.5/h vs 4.5/h, P < 0.05), L-arginine (17.5/h vs 4.5/h, P < 0.05) and naloxone(12/h vs 4.5/h, P < 0.05). On the contrary, phaclofen (6/h vs 4.5/h, P > 0.05) and tacrine (9.5/h vs 4.5/h, P > 0.05) did not influence it.

CONCLUSION: Electric acupoint stimulation at Neiguan significantly inhibits the frequency of TLESR and the rate of common cavity during TLESR in cats. This effect appears to act on the brain stem, and may be mediated through nitric oxide (NO), CCK-A receptor and mu-opioid receptors. But the GABAB receptor and acetylcholine may not be involved in it. (c) 2007 WJG. All rights reserved.

语种英语
WOS记录号WOS:000249462300012
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68256
专题北京大学第一临床医学院_消化科
作者单位Peking Univ First Hosp, Dept Gastroenterol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Wang, Chi,Zhou, De-Feng,Shuai, Xiao-Wei,et al. Effects and mechanisms of electroacupuncture at PC6 on frequency of transient lower esophageal sphincter relaxation in cats[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2007,13(36):4873-4880.
APA Wang, Chi,Zhou, De-Feng,Shuai, Xiao-Wei,Liu, Jian-Xiang,&Xie, Peng-Yan.(2007).Effects and mechanisms of electroacupuncture at PC6 on frequency of transient lower esophageal sphincter relaxation in cats.WORLD JOURNAL OF GASTROENTEROLOGY,13(36),4873-4880.
MLA Wang, Chi,et al."Effects and mechanisms of electroacupuncture at PC6 on frequency of transient lower esophageal sphincter relaxation in cats".WORLD JOURNAL OF GASTROENTEROLOGY 13.36(2007):4873-4880.
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