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学科主题临床医学
Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina
Chen, Li1; Qi, Yun2; Yang, Xinguang1
关键词Crocin Oxidative stress Ischemia/reperfusion Retinal ganglion cells
刊名OPHTHALMIC RESEARCH
2015
DOI10.1159/000439026
54期:3页:157-168
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]ISCHEMIA-REPERFUSION INJURY ; ACTIVATED PROTEIN-KINASES ; INDUCED DIABETIC-RATS ; CEREBRAL-ISCHEMIA ; ENDOTHELIAL-CELLS ; INDUCED APOPTOSIS ; SAFFRON EXTRACT ; SATIVUS L. ; DAMAGE ; ANTIOXIDANTS
英文摘要

Aims: Crocin, a pharmacologically active component of Crocus sativus L. (saffron), has been reported to be useful in the treatment of oxidative stress injury. In the present study, we investigated the antioxidative effect of crocin and the change of the ERK signaling pathway on rat retina induced by ischemia/reperfusion (IR) injury. Methods: Crocin was pretreated 30 min before and once daily after retinal IR injury by intraperitoneal injection. The retinal morphological damage was observed by hematoxylin and eosin (HE) staining. The number of retinal ganglion cells (RGCs) was counted by Brn-3a immunofluorescence staining. The antiapoptotic effect of crocin was determined by detecting cleaved caspase-3 protein levels by means of Western blot and immunohistochemical analysis. Furthermore, retinas were also used for the determination of malondialdehyde (MDA) levels, glutathione (GSH) levels, total superoxide dismutase (T-SOD), and reactive oxygen species (ROS). The phosphorylated ERK (p-ERK) protein level was determined by Western blot and immunohistochemical analysis. Results: Our results showed that crocin treatment (50 mg/kg) significantly inhibited the decrease of retinal thickness through HE staining and protected RGCs from decreasing. Compared with the IR + vehicle group, crocin treatment significantly decreased cleaved caspase-3 and p-ERK protein expression by Western blot analysis and immunohistochemistry. Immunohistochemical staining for cleaved caspase-3 and p-ERK in the retinal sections showed positive cells were present in the RGC layer and inner nuclear layer after IR injury. Similarly, crocin (50 mg/kg) treatment also significantly increased the level of activity of GSH, enhanced the activity of T-SOD, and decreased the activity level of ROS and MDA after IR injury. Conclusions: These findings demonstrated that crocin treatment could notably protect the retina from damage induced by IR. It might be related to crocin antioxidant and antiapoptotic properties in the retina. (C) 2015 S. Karger AG, Basel

语种英语
WOS记录号WOS:000364176600009
项目编号81041093 ; 81273902
资助机构National Natural Science Foundation of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68257
专题北京大学第二临床医学院_眼科
作者单位1.Xi An Jiao Tong Univ, Guangren Hosp, Xian Hosp 4, Dept Ophthalmol, Xian, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100871, Peoples R China
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Chen, Li,Qi, Yun,Yang, Xinguang. Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina[J]. OPHTHALMIC RESEARCH,2015,54(3):157-168.
APA Chen, Li,Qi, Yun,&Yang, Xinguang.(2015).Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina.OPHTHALMIC RESEARCH,54(3),157-168.
MLA Chen, Li,et al."Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina".OPHTHALMIC RESEARCH 54.3(2015):157-168.
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