北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina
作者: Chen, Li1; Qi, Yun2; Yang, Xinguang1
关键词: Crocin ; Oxidative stress ; Ischemia/reperfusion ; Retinal ganglion cells
刊名: OPHTHALMIC RESEARCH
发表日期: 2015
DOI: 10.1159/000439026
卷: 54, 期:3, 页:157-168
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: ISCHEMIA-REPERFUSION INJURY ; ACTIVATED PROTEIN-KINASES ; INDUCED DIABETIC-RATS ; CEREBRAL-ISCHEMIA ; ENDOTHELIAL-CELLS ; INDUCED APOPTOSIS ; SAFFRON EXTRACT ; SATIVUS L. ; DAMAGE ; ANTIOXIDANTS
英文摘要:

Aims: Crocin, a pharmacologically active component of Crocus sativus L. (saffron), has been reported to be useful in the treatment of oxidative stress injury. In the present study, we investigated the antioxidative effect of crocin and the change of the ERK signaling pathway on rat retina induced by ischemia/reperfusion (IR) injury. Methods: Crocin was pretreated 30 min before and once daily after retinal IR injury by intraperitoneal injection. The retinal morphological damage was observed by hematoxylin and eosin (HE) staining. The number of retinal ganglion cells (RGCs) was counted by Brn-3a immunofluorescence staining. The antiapoptotic effect of crocin was determined by detecting cleaved caspase-3 protein levels by means of Western blot and immunohistochemical analysis. Furthermore, retinas were also used for the determination of malondialdehyde (MDA) levels, glutathione (GSH) levels, total superoxide dismutase (T-SOD), and reactive oxygen species (ROS). The phosphorylated ERK (p-ERK) protein level was determined by Western blot and immunohistochemical analysis. Results: Our results showed that crocin treatment (50 mg/kg) significantly inhibited the decrease of retinal thickness through HE staining and protected RGCs from decreasing. Compared with the IR + vehicle group, crocin treatment significantly decreased cleaved caspase-3 and p-ERK protein expression by Western blot analysis and immunohistochemistry. Immunohistochemical staining for cleaved caspase-3 and p-ERK in the retinal sections showed positive cells were present in the RGC layer and inner nuclear layer after IR injury. Similarly, crocin (50 mg/kg) treatment also significantly increased the level of activity of GSH, enhanced the activity of T-SOD, and decreased the activity level of ROS and MDA after IR injury. Conclusions: These findings demonstrated that crocin treatment could notably protect the retina from damage induced by IR. It might be related to crocin antioxidant and antiapoptotic properties in the retina. (C) 2015 S. Karger AG, Basel

语种: 英语
所属项目编号: 81041093 ; 81273902
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000364176600009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68257
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Xi An Jiao Tong Univ, Guangren Hosp, Xian Hosp 4, Dept Ophthalmol, Xian, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100871, Peoples R China

Recommended Citation:
Chen, Li,Qi, Yun,Yang, Xinguang. Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina[J]. OPHTHALMIC RESEARCH,2015,54(3):157-168.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Li]'s Articles
[Qi, Yun]'s Articles
[Yang, Xinguang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Li]‘s Articles
[Qi, Yun]‘s Articles
[Yang, Xinguang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace