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学科主题: 临床医学
题名:
Articular cartilage repair using dedifferentiated articular chondrocytes and bone morphogenetic protein 4 in a rabbit model of articular cartilage defects
作者: Lin, Lin1; Zhou, Chunyan2; Wei, Xuelei1; Hou, Yu1; Zhao, Liheng1; Fu, Xin1; Zhang, Jiying1; Yu, Changlong1
刊名: ARTHRITIS AND RHEUMATISM
发表日期: 2008-04-01
DOI: 10.1002/art.23380
卷: 58, 期:4, 页:1067-1075
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: IN-VITRO ; CHONDROGENIC DIFFERENTIATION ; PHENOTYPIC STABILITY ; CHONDRAL DEFECTS ; ALGINATE BEADS ; STEM-CELLS ; VIVO ; CULTURE ; KNEE ; TRANSPLANTATION
英文摘要:

Objective. To observe redifferentiation of dedifferentiated chondrocytes after transplantation into the joint, and to evaluate the ability of dedifferentiated chondrocytes transduced with adenovirus containing bone morphogenetic protein 4 (BMP-4) to redifferentiate in vitro and in vivo in a rabbit model of articular cartilage defects.

Methods. Monolayer and,pellet culture systems were used to evaluate the redifferentiation of dedifferentiated chondrocytes transduced with BMP-4. A rabbit model of partial-thickness articular cartilage defects was used to evaluate cartilage repair macroscopically and histologically, 6 and 12 weeks after transplantation with first-passage, fifth-passage, or transduced fifth-passage chondrocytes. Histologic grading of the repaired tissue was performed. Expression of BMP-4 and the ability of transplanted cells to recover a chondrocytic phenotype were also assessed.

Results. BMP-4-expressing dedifferentiated chondrocytes recovered a chondrocytic phenotype in vitro. After transplantation into the joint, some of the dedifferentiated chondrocytes in the defect sites could undergo redifferentiation and formed matrix that displayed positive toluidine blue staining for glycosaminoglycans. Histologic scores of the regenerative tissue revealed significantly better cartilage repair in rabbits transplanted with BMP-4-expressing cells than in the other treatment groups. Staining with toluidine blue revealed expression of BMP-4 in the cells and in the matrix surrounding the cells.

Conclusion. Some dedifferentiated chondrocytes can redifferentiate after transplantation into the load-bearing joint. BMP-4 can be used to induce redifferentiation of dedifferentiated chondrocytes in vitro and in vivo, which could help enhance articular cartilage repair.

语种: 英语
WOS记录号: WOS:000255345200018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68268
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Sport Med, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Beijing, Peoples R China

Recommended Citation:
Lin, Lin,Zhou, Chunyan,Wei, Xuelei,et al. Articular cartilage repair using dedifferentiated articular chondrocytes and bone morphogenetic protein 4 in a rabbit model of articular cartilage defects[J]. ARTHRITIS AND RHEUMATISM,2008,58(4):1067-1075.
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