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学科主题临床医学
Articular cartilage repair using dedifferentiated articular chondrocytes and bone morphogenetic protein 4 in a rabbit model of articular cartilage defects
Lin, Lin1; Zhou, Chunyan2; Wei, Xuelei1; Hou, Yu1; Zhao, Liheng1; Fu, Xin1; Zhang, Jiying1; Yu, Changlong1
刊名ARTHRITIS AND RHEUMATISM
2008-04-01
DOI10.1002/art.23380
58期:4页:1067-1075
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
研究领域[WOS]Rheumatology
关键词[WOS]IN-VITRO ; CHONDROGENIC DIFFERENTIATION ; PHENOTYPIC STABILITY ; CHONDRAL DEFECTS ; ALGINATE BEADS ; STEM-CELLS ; VIVO ; CULTURE ; KNEE ; TRANSPLANTATION
英文摘要

Objective. To observe redifferentiation of dedifferentiated chondrocytes after transplantation into the joint, and to evaluate the ability of dedifferentiated chondrocytes transduced with adenovirus containing bone morphogenetic protein 4 (BMP-4) to redifferentiate in vitro and in vivo in a rabbit model of articular cartilage defects.

Methods. Monolayer and,pellet culture systems were used to evaluate the redifferentiation of dedifferentiated chondrocytes transduced with BMP-4. A rabbit model of partial-thickness articular cartilage defects was used to evaluate cartilage repair macroscopically and histologically, 6 and 12 weeks after transplantation with first-passage, fifth-passage, or transduced fifth-passage chondrocytes. Histologic grading of the repaired tissue was performed. Expression of BMP-4 and the ability of transplanted cells to recover a chondrocytic phenotype were also assessed.

Results. BMP-4-expressing dedifferentiated chondrocytes recovered a chondrocytic phenotype in vitro. After transplantation into the joint, some of the dedifferentiated chondrocytes in the defect sites could undergo redifferentiation and formed matrix that displayed positive toluidine blue staining for glycosaminoglycans. Histologic scores of the regenerative tissue revealed significantly better cartilage repair in rabbits transplanted with BMP-4-expressing cells than in the other treatment groups. Staining with toluidine blue revealed expression of BMP-4 in the cells and in the matrix surrounding the cells.

Conclusion. Some dedifferentiated chondrocytes can redifferentiate after transplantation into the load-bearing joint. BMP-4 can be used to induce redifferentiation of dedifferentiated chondrocytes in vitro and in vivo, which could help enhance articular cartilage repair.

语种英语
WOS记录号WOS:000255345200018
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68268
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Inst Sport Med, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Lin, Lin,Zhou, Chunyan,Wei, Xuelei,et al. Articular cartilage repair using dedifferentiated articular chondrocytes and bone morphogenetic protein 4 in a rabbit model of articular cartilage defects[J]. ARTHRITIS AND RHEUMATISM,2008,58(4):1067-1075.
APA Lin, Lin.,Zhou, Chunyan.,Wei, Xuelei.,Hou, Yu.,Zhao, Liheng.,...&Yu, Changlong.(2008).Articular cartilage repair using dedifferentiated articular chondrocytes and bone morphogenetic protein 4 in a rabbit model of articular cartilage defects.ARTHRITIS AND RHEUMATISM,58(4),1067-1075.
MLA Lin, Lin,et al."Articular cartilage repair using dedifferentiated articular chondrocytes and bone morphogenetic protein 4 in a rabbit model of articular cartilage defects".ARTHRITIS AND RHEUMATISM 58.4(2008):1067-1075.
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