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学科主题: 临床医学
题名:
Loss of STAG2 causes aneuploidy in normal human bladder cells
作者: Li, X.1,2; Zhang, T. W.1; Tang, J. L.2; Fa, P. P.4; Lu, J. X.2; Qi, F. M.2; Cai, Z. M.3; Liu, C. X.1; Sun, X. J.2,3
关键词: Urologic neoplasms ; Stromal antigen 2 ; Aneuploidy ; Bladder cancer ; Cohesin complex
刊名: GENETICS AND MOLECULAR RESEARCH
发表日期: 2015
DOI: 10.4238/2015.March.30.24
卷: 14, 期:1, 页:2638-2646
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]: SISTER-CHROMATID COHESION ; CANCER ; MUTATIONS ; COMPLEX ; GENE ; INACTIVATION ; COMPONENTS ; HANDCUFF ; MEIOSIS ; MODEL
英文摘要:

The aim of this study was to determine how the function of human stromal antigen 2 (STAG2) plays an important role in proper chromosome separation. STAG2 mRNA in normal bladder cells and bladder tumor cells was evaluated by RT-PCR. The protein levels of STAG2 in normal bladder cells and bladder tumor cells were determined by western blot. A cell proliferation assay was used to measure the growth of tumor cells and STAG2-inhibited normal cells, and STAG2-inhibited normal cells were subjected to karyotype analysis. Both STAG-2 mRNA and protein expression levels were lower in bladder cancer cells compared to the controls. Knockdown of STAG2 caused aneuploidy in normal bladder cells, leading to a decreased expression of the cohesin complex components SMC1, SMC3 and RAD21, but there was no obvious effect of STAG2 knockdown on cell proliferation. Our study indicated that abnormal expression of STAG2 could cause aneuploidy in normal bladder cells.

语种: 英语
所属项目编号: NSFC 81101584 ; 81372507 ; 2014DFA31050 ; KQC201106020032A ; JCYJ20120614455816670 ; 2014CB745200 ; 2012AA02A201 ; 2012AA02A208
项目资助者: National Nature Science Foundation of China ; International S&amp ; T Cooperation Program of China ; Shenzhen Peacock Foundation ; Shenzhen Basic Research Key Project ; Development Program for Basic Research of China "973" Program ; Chinese High-Tech "863" Program
WOS记录号: WOS:000357192700116
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68273
Appears in Collections:北京大学深圳医院_泌尿外科_期刊论文

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作者单位: 1.Southern Med Univ, Affiliated Hosp 2, Zhujiang Hosp, Dept Urol, Guangzhou, Guangdong, Peoples R China
2.Shenzhen Univ, Affiliated Hosp 1, Biobank Shenzhen Peoples Hosp 2, Shenzhen Tumor Immunogene Therapy Clin Applicat E, Shenzhen, Guangdong, Peoples R China
3.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Natl Reg Key Technol Engn Lab Clin Applicat Canc, Shenzhen, Guangdong, Peoples R China
4.Peking Univ, Shenzhen Hosp, Inst Urol, Shenzhen PKU HKUST Med Ctr,Male Reprod Med & Gene, Shenzhen, Guangdong, Peoples R China

Recommended Citation:
Li, X.,Zhang, T. W.,Tang, J. L.,et al. Loss of STAG2 causes aneuploidy in normal human bladder cells[J]. GENETICS AND MOLECULAR RESEARCH,2015,14(1):2638-2646.
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