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学科主题: 临床医学
题名:
Effect of embryo culture media on percentage of males at birth
作者: Zhu, Jinliang1,2,3; Zhuang, Xinjie1,2,3; Chen, Lixue1,2,3; Liu, Ping1,2,3; Qiao, Jie1,2,3
关键词: sex at birth ; culture medium ; singleton gestation ; ICSI ; IVF
刊名: HUMAN REPRODUCTION
发表日期: 2015-05-01
DOI: 10.1093/humrep/dev049
卷: 30, 期:5, 页:1039-1045
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: HUMAN SEX-RATIO ; IN-VITRO ; PERICONCEPTIONAL SMOKING ; BLASTOCYST TRANSFER ; NATURAL VARIATION ; OFFSPRING SEX ; FEMALE RATIO ; GLUCOSE ; STAGE ; IMBALANCE
英文摘要:

STUDY QUESTION: Does embryo culture medium influence the percentage of males at birth?

SUMMARY ANSWER: The percentage of males delivered after ICSI cycles using G5 (TM) medium was statistically significantly higher than after cycles where Global, G5 (TM) PLUS, and Quinn′s Advantage Media were used.

WHAT IS KNOWN ALREADY: Male and female embryos have different physiologies during preimplantation development. Manipulating the energy substrate and adding growth factors have a differential impact on the development of male and female embryos.

STUDY DESIGN, SIZE AND DURATION: This was a retrospective analysis of the percentage of males at birth, and included 4411 singletons born from fresh embryo transfer cycles between January 2011 and August 2013 at the Center for Reproductive Medicine of Third Hospital Peking University.

PARTICIPANTS/MATERIALS, SETTING, AND METHODS: Only singleton gestations were included. Participants were excluded if preimplantation genetic diagnosis, donor oocytes and donor sperm were used. The database between January 2011 and August 2013 was searched with unique medical record number, all patients were present in the database with only one cycle. Demographics, cycle characteristics and the percentage of male babies in the four culture media groups were compared with analysis of variance or chi(2) tests. Multivariable logistic regression was done to determine the association between the sex at birth and culture media after adjusting for other confounding factors, including parental age, parental BMI, type of infertility, parity, number of embryos transferred, number of early gestational sacs, cycles with testicular sperm aspiration (TESA)/percutaneous epididymal sperm aspiration (PESA)/testicular sperm extraction (TESE), number of oocytes retrieved, cycles with blastocyst transfers, and gestational age within ICSI group.

MAIN RESULTS AND THE ROLE OF CHANCE: Within the IVF group, the percentage of males at birth for G5 (TM), Global, Quinn′s and G5 (TM) PLUS media were comparable (P > 0.05); however, within the ICSI group, the percentage of male babies in cycles using G5 (TM) (56.1%) was statistically significantly higher than in cycles that used Global (47.2%; P = 0.003), G5 (TM) PLUS (47.7%; P = 0.005) or Quinn′s media (45.0%; P = 0.009). There were no statistically significant differences in the percentage of males at birth between cycles that used Global, G5 (TM) PLUS and Quinn′s media (P > 0.05). Multivariable logistic regression indicated that culture media (G5 (TM) versus Global, G5 (TM) PLUS, and Quinn′s) were significantly associated with the sex at birth (P = 0.008) after adjusting for parental age, parental BMI, type of infertility, parity, number of embryos transferred, number of early gestational sacs, cycles with TESA/PESA/TESE, number of oocytes retrieved, cycles with blastocyst transfers, and gestational age.

LIMITATIONS AND REASONS FOR CAUTION: This study was not a randomized controlled trial and allocation of treatment cycles over the four media was not completely at random. Cigarette smoking was not included in the current study because this confounding factor was not registered in our database. Moreover, intra-variability of sperm selection between the five embryologists may directly affect the percentage of males.

WIDER IMPLICATIONS OF THESE FINDINGS: Our study suggests that human embryogenesis responds differently to G5 (TM), Global, G5 (TM) PLUS and Quinn′s Advantage Medium. This finding can be generalized to other commercial culture media.

语种: 英语
所属项目编号: 81300483 ; 81200466
项目资助者: National Natural Science Foundation of China for Young Scholars
WOS记录号: WOS:000354792100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68305
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Reprod Med Ctr, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
2.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China

Recommended Citation:
Zhu, Jinliang,Zhuang, Xinjie,Chen, Lixue,et al. Effect of embryo culture media on percentage of males at birth[J]. HUMAN REPRODUCTION,2015,30(5):1039-1045.
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