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学科主题: 病理学
题名:
TAK1赖氨酸158位点发生Lys63连接多聚泛素化在辐射诱导NF-κB激活过程中的作用
其他题名: Roel of Lys63-linked TAK1 poly-ubiquitination at lysine 158 in radiation-induced NF-κB activity
作者: 朱翔; 曹宝山; 张照辉; 马力文; 苏旭; 梁莉
关键词: 辐射损伤 ; 转化生长因子β激酶1 ; 多聚泛素化 ; NF-κB ; 信号通路 ; Radiation effect ; Transforming growth factor-β-activating kinase 1 ; Polyubiquitination ; Nuclear factor-kappa B ; Signaling pathway
刊名: 中华放射医学与防护杂志
发表日期: 2013
DOI: 10.3760/cma.j.issn.0254-5098.2013.03.004
卷: 33, 期:3, 页:235-238
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 研究转化生长因子激酶1(TAK1) Lys 63连接多聚泛素化在辐射导致的NF-κB激活过程中起到的作用及其作用位点.方法 通过基因瞬时转染技术将FLAG-TAK1和HA-Ub-K63质粒共同转染至HEK-293T细胞,应用免疫沉淀和免疫印迹技术研究辐射对TAK1泛素化的影响;用稳定转染TAK1表达的HeLa细胞,证实辐射对TAK1泛素化的影响.通过免疫印迹技术,比较分析辐射对稳定转染TAK1 K158R的MEF细胞与TAK1野生型MEF细胞比较分析辐射对它表达IKKs、p38及JNK的影响,以证明辐射诱导的TAK1-Lys 63多聚泛素化的位点是否在TAK1赖氨酸158位点.结果 瞬时转染的HEK-293T细胞接受辐射后1h开始出现明显的TAK1泛素化表现,2h更明显.在稳定表达FLAG-TAK1的HeLa细胞系中得到证实.与TAK1野生型MEF细胞相比,TAK1 K158R突变的MEF细胞中辐射诱导的IKKs和p38磷酸化作用减低.结论 TAK1多聚泛素化在辐射导致的NF-κB激活过程中起到关键作用,辐射所致TAK1-Lys 63连接多聚泛素化发生在TAK1的赖氨酸158位点. Objective To study the role of the transforming growth factor-β-activating kinase 1 (TAK1) poly-ubiquitination in radiation-induced NF-κB activation.Methods FLAG-TAK1 and HA-UbK63 plasmids were transiently transfected into HEK-293T cells.The radiation effect on TAK1 ubiquitination was detected by immunoprecipitation and Western blot,which was further confirmed in FLAG-TAK1 HeLa stable cell line.Results At 1 h after irradiation,Lys 63-1ink TAK1 ubiquitination was induced in the HEK-293T cells with plasmids transfection.This result was further confirmed by using a FLAG-TAK1 stably expressed HeLa cell line.Radiation-induced IKKs and p38 phosphorylation were greatly impaired in MEFs reconstituted with TAK1 K158R mutant compared to ones with wild-type TAK1.Conclusions Lys63-1inked TAK1 poly-ubiquitination at Lys-58 plays a key role in the process of radiation-induced NF-κB activation.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68336
Appears in Collections:北京大学第三临床医学院_病理科_期刊论文

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作者单位: 1.北京大学第三医院病理科,100191
2.北京大学第三医院肿瘤化疗与放射病科,100191
3.中国疾病预防控制中心辐射防护与核安全医学所

Recommended Citation:
朱翔,曹宝山,张照辉,等. TAK1赖氨酸158位点发生Lys63连接多聚泛素化在辐射诱导NF-κB激活过程中的作用[J]. 中华放射医学与防护杂志,2013,33(3):235-238.
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