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学科主题: 整形医学
题名:
基于文献挖掘的增生性瘢痕相关基因的生物信息学分析
其他题名: Literature mining and bioinformatic analysis of dysregulated genes in hypertrophic scar
作者: 黄琛; 李博仑; 秦泽莲
关键词: 计算生物学 ; 瘢痕,肥大性 ; 皮肤 ; Computational biology ; Cicatrix,hypertrophic ; Skin
刊名: 中华整形外科杂志
发表日期: 2011
DOI: 10.3760/cma.j.issn.1009-4598.2011.06.013
卷: 27, 期:6, 页:453-460
收录类别: 中国科技核心期刊 ; 中文核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的 比较增生性瘢痕(hypertrophic scar,HS)与正常皮肤的基因表达差异,从分子水平揭示HS的发病机制,为其临床防治提供新思路.方法 利用PubMed公共数据库文献挖掘HS与正常皮肤的差异表达基因,确定HS相关基因,并进行蛋白-蛋白相互作用网络、生物学通路、GeneOntology(基因本体)和功能注释聚类等生物信息学分析.结果 筛选HS相关基因55个(上调基因46个,下调基因9个).51个基因构成蛋白-蛋白相互作用网络,其中上调基因TGFB1、FN1、JUN、COL1A1、CTGF、VEGFA、FOS、COL3A1、IGF1、IL4、PELO、SMAD2、TIMP1、PCNA、ITGA4和下调基因ITGB1和DCN为此网络中心节点.HS相关基因参与黏着斑形成、整合素信号转导和肿瘤相关等生物学通路.并在细胞表面受体介导和胞内信号转导、组织发育与形态发生、细胞凋亡与增殖、大分子合成与代谢等生物过程,钙离子结合、双链DNA结合、启动子结合、肝素结合和促分裂素原活化蛋白激酶(MAPK)激活等分子功能中起着重要作用.功能注释聚类显示,HS相关基因多与表皮组织发育,血管生成,以及细胞凋亡调控有关.结论 TGFB1、FN1、JUN等关键基因,以及表皮组织发育,血管生成,以及细胞外基质-整合素-黏着斑相关的生物学通路、生物学过程和分子功能在HS的发生发展中可能起着重要作用,对其进一步分析有利于揭示HS的发病机制,并为临床治疗提供新的靶点. Objective To explore the pathogenesis mechanism of hypertrophic scar (HS) and the effective means for its clinical treatment,the difference of the gene expressions between HS and normal skin was compared.Methods The differentially expressed genes between HS and normal skin were obtained by mining PubMed.The dysregulated genes in HS were analyzed by a series of bioinformatics methods,including protein-protein interaction networks,pathways,Gene Ontology and functional annotation clustering analysis.Results A total of 55 dysregulated genes in HS was identified (46 up-regulated genes and 9 down-regulated genes ).Fifty-one genes were found to encode proteins with interaction network,including up-regulated genes TGFB1,FN1,JUN,COL1A1,CTGF,VEGFA,FOS,COL3A1,IGF1,II4,PELO,SMAD2,TIMP1,PCNA,and ITGA4 and down-regulated genes ITGB1 and DCN as the central nodes for this network.The dysregulated genes in HS involved in a variety of biological pathways,such as focal adhesion formation,integrin signal transduction,and tumor formation.Furthermore,the dysregulated genes in HS played the important roles in biological processes of cell surface receptor linked signal transduction,tissue development,cell proliferation and apoptosis,and macromolecule biosynthetic process,as well as in molecular function of calcium ion binding,double-stranded DNA binding,heparin binding,promoter binding and MAP kinase activity.The results of functional annotation clustering analysis revealed that the dysregulated genes in HS involved in epidermis development,angiogenesis,and apoptosis.Conclusion Such key genes as TGFB1,FN1,and JUN,along with the pathways,biological processes and molecular functions involving epidermis development,angiogenesis,and extracellular matrixintegrin-focal adhesion signal transduction may play the important roles in the development of HS.The investigations of the dysregulated genes in HS could provide the new targets for clinical treatment.
语种: 中文
原文出处: 查看原文
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68602
Appears in Collections:北京大学第三临床医学院_成形外科_期刊论文

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作者单位: 1.100191北京,北京大学第三医院成形外科
2.100191北京,北京大学第三医院中心实验室
3.北京大学第三医院成形外科,北京,100191

Recommended Citation:
黄琛,李博仑,秦泽莲. 基于文献挖掘的增生性瘢痕相关基因的生物信息学分析[J]. 中华整形外科杂志,2011,27(6):453-460.
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