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成骨细胞特异性因子2periostin及相关因子在大鼠全层缺损皮肤创面愈合过程中的表达
其他题名The expressions of periostin and the related factors during healing process of full-thickness cutaneous wound in rat
侯俊杰; 聂芳菲; 李博仑; 赵霞; 秦泽莲; 马勇光; 李健宁
关键词成骨细胞特异性因子 创面愈合 血管生成素-1 血管内皮生长因子 血管内皮生长因子受体2 Periostin Wound healing Angiopoietin-1 Vascular endothelial growth factor Vascularendothelial growth factor receptor-2
刊名中国危重病急救医学
2012
DOI10.3760/cma.j.issn.1003-0603.2012.06.005
24期:6页:334-337
收录类别中文核心期刊
文章类型Journal Article
摘要目的 观察大鼠皮肤缺损创面愈合过程中成骨细胞特异性因子2 periostin( PN)与血管生成素-1(Ang-1)、血管内皮生长因子(VEGF)及其受体2[VEGFR-2/胎肝激酶-1(FLK-1)]在创面及创缘周围皮肤的表达及对创面愈合的影响.方法 48只成年雄性SD大鼠按随机数字表法分成6组,每组8只.在大鼠背中部脊柱两侧各制作一个2cm×2cm全层皮肤缺损创面,分别于术后1、4、7、10、14、21d创面取材,行病理组织学检查及免疫组化染色检测创面PN、Ang-1、VEGF、FLK-1的表达;并以正常皮肤的表达量作为对照组.结果 创面局部组织中各因子的表达量在术后均迅速升高.与对照组比较,创面PN的表达量在术后1d即增加了234.4%,之后持续升高,至7d增加了597.9%并达到峰值,其后缓慢下降,21 d时增长率为280.9%,仍维持在较高水平(均P<0.05);Ang-1的表达量在术后1d增加了128.1%,4d增加了327.5%并达到峰值,然后逐渐下降,14d的增长率仅为80.5%,之后轻度升高(均P<0.05);VEGF的表达量在术后7d增加了165.8%并达到峰值,而后下降并伴有小幅波动(均P<0.05):FLK-1术后1d增加了56.1%并维持这一水平,至7d增加了70.1%达峰值(均P<0.05),10d后缓慢下降(均P>0.05).结论 大鼠皮肤创面愈合过程中,PN、Ang-1、VEGF及其受体FLK-1蛋白均高表达,但各因子的表达高峰和持续时间有所不同.PN的表达量增幅最高且始终维持较高水平至创面表皮化后;说明PN、Ang-1、VEGF、FLK-1共同参与了大鼠皮肤创面的愈合,PN在创面愈合的整个过程巾发挥着重要作用. Objective To observe the expression of osteoblast-specific factor 2 (periostin,PN),angiopoietin- 1 ( Ang- 1 ),vascular endothelial growth factor ( VEGF ) and vascular endothelial growth factor receptor-2 [ VEGFR-2/fetal liver kinase-1 ( FLK-1 )]in wound surface and its peripheral skin,and their effects on wound healing in rats.Methods Forty-eight Sprague-Dawley (SD) rats were randomly divided into six groups,with 8 rats in each group.An area of 2 cm × 2 cm full-thickness skin was excised on both sides of the back of rats.Specimens from wounds were obtained on 1,4,7,10,14,21 days after operation,and histological evaluation and immunohistochemical staining of PN,Ang-1,VEGF and FLK-1 were made to determine their expression levels.Normal skin specimens wereobtained as normal controls.Results The expressions of PN,Ang-1,VEGF and FLK-1 were significantly increased in wound surface after operation.Compared with the skin of normal controls,the expression of PN in the tissues of wound increased by 234.4% on the 1 st day,and then increased continuously up to 597.9% on the 7th day (reaching the peak )after operation,followed by a decrease,the increase rate was 280.9% on the 21st day,and still remained at a high level (all P<0.05 ).The expression of Ang-1 in the tissue of wound increased by 128.1% on the 1st day and 327.5% on the 4th day (reaching the peak),and then,it was gradually decreased.The increase rate was only 80.5% on the 14th day and it rose slightly later ( all P<0.05 ).The expression of VEGF in the tissues of wound reached the peak ( 165.8% ) on the 7th day.Then it decreased with a slight fluctuation (all P<0.05 ).The expression of FLK- 1 in the tissues of wound was increased by 56.1% on the 1st day,and the level remained.It reached the peak by an increase of 70.1% on the 7th day (both P<0.05 ).Then,it was lowered after the 10th day ( all P>0.05 ).Conclusions The expressions of PN,Ang-1,VEGF,FLK-1 were obviously increased during healing of skin wound,with different peaking time and expressing rates.The increase in expression of PN showed the longest duration and highest peak value.The PN,Ang-1,VEGF,FLK-1 all play a role in the wound healing process,and PN might play an important role during the healing process of a full-thickness cutaneous wound.
语种中文
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文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/68603
专题北京大学第三临床医学院_成形外科
作者单位100191北京大学第三医院成形外科
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侯俊杰,聂芳菲,李博仑,等. 成骨细胞特异性因子2periostin及相关因子在大鼠全层缺损皮肤创面愈合过程中的表达[J]. 中国危重病急救医学,2012,24(6):334-337.
APA 侯俊杰.,聂芳菲.,李博仑.,赵霞.,秦泽莲.,...&李健宁.(2012).成骨细胞特异性因子2periostin及相关因子在大鼠全层缺损皮肤创面愈合过程中的表达.中国危重病急救医学,24(6),334-337.
MLA 侯俊杰,et al."成骨细胞特异性因子2periostin及相关因子在大鼠全层缺损皮肤创面愈合过程中的表达".中国危重病急救医学 24.6(2012):334-337.
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