北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 成形外科  > 期刊论文
学科主题: 整形医学
题名:
丹参酮ⅡA 磺酸钠在低氧下对瘢痕疙瘩成纤维细胞增殖和促纤维化因子表达的影响
其他题名: Effects of Sodium Tanshinone ⅡA Sulfonate Under Hypoxia on Proliferation of Keloid Fibroblasts and Expression of Pro-Fibrogenic Factors
作者: 康春福; 陈斌; 秦泽莲; 孙艳; 李艳芳; 于东宁; 安阳; 毕洪森
关键词: 丹参酮ⅡA磺酸钠 ; 瘢痕疙瘩成纤维细胞 ; 低氧 ; 增殖 ; 基因表达 ; Sodium tanshinoneⅡA sulfonate ; Keloid fibroblast ; Hypoxia ; Proliferation ; Gene expression
刊名: 中国微创外科杂志
发表日期: 2015
DOI: 10.3969/j.issn.1009-6604.2015.07.021
期: 7, 页:649-654
收录类别: 中国科技核心期刊 ; CSCD
文章类型: Journal Article
摘要: 目的:研究常氧和低氧下,丹参酮ⅡA磺酸钠( sodium tanshinoneⅡA sulfonate,STS)对瘢痕疙瘩成纤维细胞( keloid fibroblasts, KFs)增殖和促纤维化因子表达的影响,探讨STS治疗瘢痕疙瘩的可能作用。方法原代培养5例KFs,将细胞分组,分别在常氧(21%O2)和低氧(2%O2)下用不同剂量的STS(0、50、100、200、400、800μmol/L)干预48 h,采用CCK-8方法检测细胞增殖活力,同时在倒置相差显微镜下观察细胞的形态学变化。用不同剂量的STS(0、100、200μmol/L)同样干预48 h,实时定量PCR检测低氧诱导因子1α(HIF-1α)、转化生长因子β1(TGF-β1)、Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)、血管内皮生长因子(VEGF)、成骨细胞特异因子2(Periostin)的mRNA表达情况,蛋白质印迹法检测HIF-1α、TGF-β1蛋白的表达情况。结果低氧与常氧下的KFs增殖活力无明显差异;STS在常氧和低氧下明显抑制KFs增殖(P<0.01),其有剂量依赖性,在低氧条件下STS的起效剂量比常氧下的高。低氧能够引起HIF-1α的mRNA和蛋白分别增加0.606(P=0.037)、0.950(P=0.002),VEGF mRNA上调7.256(P=0.043),Periostin mRNA上调6.285(P=0.006),TGF-β1蛋白增加1.641(P=0.011),对ColⅠ和ColⅢ的mRNA的表达无明显影响。常氧下,200μmol/L的STS增加HIF-1α和Periostin的mRNA表达分别为0.750(P=0.015)和8.553(P=0.000),减少TGF-β1、ColⅠ和ColⅢ的mRNA表达分别为0.349(P=0.007)、0.320(P=0.006)、0.453(P=0.015),对TGF-β1蛋白和VEGF mRNA表达影响不明显。低氧48 h,200μmol/L的STS使HIF-1αmRNA和蛋白表达分别减少0.548(P=0.016)、0.984(P=0.001),对TGF-β1 mRNA和蛋白以及ColⅠ、ColⅢ、VEGF、Periostin的mRNA表达影响不明显。结论常氧和低氧下STS对KFs的增殖均有抑制作用,并能够影响促纤维化因子的表达,可能作为治疗瘢痕疙瘩的潜在药物。 Objective To study the effects of sodium tanshinone ⅡA sulfonate ( STS ) under normoxia and hypoxia on proliferation of keloid fibroblasts ( KFs) and expression of pro-fibrogenic factors, and to explore the potential therapeutic role of STS for keloid. Methods A total of 5 cases of KFs were cultured and then divided into different groups.The samples were treated with different doses of STS (0, 50, 100, 200, 400, and 800 μmol/L) for 48 h under normoxia (21% O2) or hypoxia (2% O2).The proliferation activity of KFs was detected with CCK-8 kit and the cell morphology was observed under inverted phase contrast microscope.After treatment of STS (0, 100, and 200μmol/L, respectively), real time PCR assay was adopted to measure the mRNA expression of hypoxia inducible factor 1α(HIF-1α), transforming growth factorβ1 (TGF-β1), collagen typeⅠ(ColⅠ), collagen typeⅢ( Col Ⅲ) , vascular endothelial growth factor ( VEGF) , and periostin ( PN) .The protein expressions of HIF-1αand TGF-β1 were examined by western blot assay. Results The proliferation activity of KFs was not significantly different between normoxia and hypoxia.The STS inhibited the proliferation of KFs in a dose-dependent manner in normoxia and hypoxia (P<0.05).The minimal effective dose in hypoxia was higher than that in normoxia.Hypoxia for 48 h changed the expressions of pro-fibrogenic factors.The mRNA and protein expressions of HIF-1αwere increased by 0.606 (P=0.037) and 0.950 (P=0.002), respectively.The mRNA expression of VEGF was increased by 7.256 (P=0.043).The mRNA expression of periostin was increased by 6.285 (P=0.006).The protein expression of TGF-β1 was increased by 1.641 (P=0.011).However, the STS did not significantly effected on the mRNA expressions of ColⅠand Col Ⅲ.Treated with STS under normoxia for 48 h, the mRNA expressions of HIF-1αand periostin were increased by 0.750 (P=0.015) and 8.553 (P=0.000), respectively.The mRNA expressions of TGF-β1, ColⅠ, and ColⅢwere decreased by 0.349 (P=0.007), 0.320 (P=0.006), and 0.453 (P=0.015), respectively.There were not significant changes in the expressions of TGF-β1 protein and VEGF mRNA (P>0.05).Treated with STS under hypoxia for 48 h, the mRNA and protein levels of HIF-1αwere decreased by 0.548 (P=0.016) and 0.984 (P=0.001), respectively.There were no significant changes in the mRNA levels of TGF-β1, ColⅠ, ColⅢ, VEGF,and periostin, as well as the protein level of TGF-β1. Conclusions The STS inhibits KFs proliferation in a dose-dependent manner and affects the expressions of pro-fibrogenic factors under both normoxia and hypoxia.The STS could be used as a candidate of therapeutic treatment against keloid.
语种: 中文
原文出处: 查看原文
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/68706
Appears in Collections:北京大学第三临床医学院_成形外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.北京大学第三医院成形外科,北京,100191
2.北京大学第三医院中心实验室,北京,100191
3.北京积水潭医院烧伤科,北京,100035

Recommended Citation:
康春福,陈斌,秦泽莲,等. 丹参酮ⅡA 磺酸钠在低氧下对瘢痕疙瘩成纤维细胞增殖和促纤维化因子表达的影响[J]. 中国微创外科杂志,2015(7):649-654.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[康春福]'s Articles
[陈斌]'s Articles
[秦泽莲]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[康春福]‘s Articles
[陈斌]‘s Articles
[秦泽莲]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace